Hfq Regulates Efflux Pump Expression and Purine Metabolic Pathway to Increase Trimethoprim Resistance in Aeromonas veronii

Aeromonas veronii (A. veronii) is a zoonotic pathogen. It causes clinically a variety of diseases such as dysentery, bacteremia, and meningitis, and brings huge losses to aquaculture. A. veronii has been documented as a multiple antibiotic resistant bacterium. Hfq (host factor for RNA bacteriophage...

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Autores principales:	Dan Wang, Hong Li, Xiang Ma, Yanqiong Tang, Hongqian Tang, Dongyi Huang, Min Lin, Zhu Liu
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Aeromonas veronii Hfq trimethoprim antibiotic resistance acrA/acrB purine pathway Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/db2dcd9052884620832db92727074e02
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spelling	oai:doaj.org-article:db2dcd9052884620832db92727074e022021-11-30T18:28:46ZHfq Regulates Efflux Pump Expression and Purine Metabolic Pathway to Increase Trimethoprim Resistance in Aeromonas veronii1664-302X10.3389/fmicb.2021.742114https://doaj.org/article/db2dcd9052884620832db92727074e022021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.742114/fullhttps://doaj.org/toc/1664-302XAeromonas veronii (A. veronii) is a zoonotic pathogen. It causes clinically a variety of diseases such as dysentery, bacteremia, and meningitis, and brings huge losses to aquaculture. A. veronii has been documented as a multiple antibiotic resistant bacterium. Hfq (host factor for RNA bacteriophage Qβ replication) participates in the regulations of the virulence, adhesion, and nitrogen fixation, effecting on the growth, metabolism synthesis and stress resistance in bacteria. The deletion of hfq gene in A. veronii showed more sensitivity to trimethoprim, accompanying by the upregulations of purine metabolic genes and downregulations of efflux pump genes by transcriptomic data analysis. Coherently, the complementation of efflux pump-related genes acrA and acrB recovered the trimethoprim resistance in Δhfq. Besides, the accumulations of adenosine and guanosine were increased in Δhfq in metabonomic data. The strain Δhfq conferred more sensitive to trimethoprim after appending 1 mM guanosine to M9 medium, while wild type was not altered. These results demonstrated that Hfq mediated trimethoprim resistance by elevating efflux pump expression and degrading adenosine, and guanosine metabolites. Collectively, Hfq is a potential target to tackle trimethoprim resistance in A. veronii infection.Dan WangDan WangHong LiXiang MaYanqiong TangHongqian TangDongyi HuangMin LinZhu LiuFrontiers Media S.A.articleAeromonas veroniiHfqtrimethoprimantibiotic resistanceacrA/acrBpurine pathwayMicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Aeromonas veronii Hfq trimethoprim antibiotic resistance acrA/acrB purine pathway Microbiology QR1-502
spellingShingle	Aeromonas veronii Hfq trimethoprim antibiotic resistance acrA/acrB purine pathway Microbiology QR1-502 Dan Wang Dan Wang Hong Li Xiang Ma Yanqiong Tang Hongqian Tang Dongyi Huang Min Lin Zhu Liu Hfq Regulates Efflux Pump Expression and Purine Metabolic Pathway to Increase Trimethoprim Resistance in Aeromonas veronii
description	Aeromonas veronii (A. veronii) is a zoonotic pathogen. It causes clinically a variety of diseases such as dysentery, bacteremia, and meningitis, and brings huge losses to aquaculture. A. veronii has been documented as a multiple antibiotic resistant bacterium. Hfq (host factor for RNA bacteriophage Qβ replication) participates in the regulations of the virulence, adhesion, and nitrogen fixation, effecting on the growth, metabolism synthesis and stress resistance in bacteria. The deletion of hfq gene in A. veronii showed more sensitivity to trimethoprim, accompanying by the upregulations of purine metabolic genes and downregulations of efflux pump genes by transcriptomic data analysis. Coherently, the complementation of efflux pump-related genes acrA and acrB recovered the trimethoprim resistance in Δhfq. Besides, the accumulations of adenosine and guanosine were increased in Δhfq in metabonomic data. The strain Δhfq conferred more sensitive to trimethoprim after appending 1 mM guanosine to M9 medium, while wild type was not altered. These results demonstrated that Hfq mediated trimethoprim resistance by elevating efflux pump expression and degrading adenosine, and guanosine metabolites. Collectively, Hfq is a potential target to tackle trimethoprim resistance in A. veronii infection.
format	article
author	Dan Wang Dan Wang Hong Li Xiang Ma Yanqiong Tang Hongqian Tang Dongyi Huang Min Lin Zhu Liu
author_facet	Dan Wang Dan Wang Hong Li Xiang Ma Yanqiong Tang Hongqian Tang Dongyi Huang Min Lin Zhu Liu
author_sort	Dan Wang
title	Hfq Regulates Efflux Pump Expression and Purine Metabolic Pathway to Increase Trimethoprim Resistance in Aeromonas veronii
title_short	Hfq Regulates Efflux Pump Expression and Purine Metabolic Pathway to Increase Trimethoprim Resistance in Aeromonas veronii
title_full	Hfq Regulates Efflux Pump Expression and Purine Metabolic Pathway to Increase Trimethoprim Resistance in Aeromonas veronii
title_fullStr	Hfq Regulates Efflux Pump Expression and Purine Metabolic Pathway to Increase Trimethoprim Resistance in Aeromonas veronii
title_full_unstemmed	Hfq Regulates Efflux Pump Expression and Purine Metabolic Pathway to Increase Trimethoprim Resistance in Aeromonas veronii
title_sort	hfq regulates efflux pump expression and purine metabolic pathway to increase trimethoprim resistance in aeromonas veronii
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/db2dcd9052884620832db92727074e02
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Hfq Regulates Efflux Pump Expression and Purine Metabolic Pathway to Increase Trimethoprim Resistance in Aeromonas veronii

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