Activation of Secondary Metabolism in Red Soil-Derived Streptomycetes via Co-Culture with Mycolic Acid-Containing Bacteria

Our previous research has demonstrated a promising capacity of streptomycetes isolated from red soils to produce novel secondary metabolites, most of which, however, remain to be explored. Co-culturing with mycolic acid-containing bacteria (MACB) has been used successfully in activating the secondar...

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Autores principales:	Kairui Wang, Ning Liu, Fei Shang, Jiao Huang, Bingfa Yan, Minghao Liu, Ying Huang
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Streptomyces</i> co-culture mycolic acid-containing bacteria (MACB) secondary metabolites (SMs) activation of natural products Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/db32fd85488f43d287bd24f1aac8385c
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spelling	oai:doaj.org-article:db32fd85488f43d287bd24f1aac8385c2021-11-25T18:24:05ZActivation of Secondary Metabolism in Red Soil-Derived Streptomycetes via Co-Culture with Mycolic Acid-Containing Bacteria10.3390/microorganisms91121872076-2607https://doaj.org/article/db32fd85488f43d287bd24f1aac8385c2021-10-01T00:00:00Zhttps://www.mdpi.com/2076-2607/9/11/2187https://doaj.org/toc/2076-2607Our previous research has demonstrated a promising capacity of streptomycetes isolated from red soils to produce novel secondary metabolites, most of which, however, remain to be explored. Co-culturing with mycolic acid-containing bacteria (MACB) has been used successfully in activating the secondary metabolism in <i>Streptomyces</i>. Here, we co-cultured 44 strains of red soil-derived streptomycetes with four MACB of different species in a pairwise manner and analyzed the secondary metabolites. The results revealed that each of the MACB strains induced changes in the metabolite profiles of 35–40 streptomycetes tested, of which 12–14 streptomycetes produced “new” metabolites that were not detected in the pure cultures. Moreover, some of the co-cultures showed additional or enhanced antimicrobial activity compared to the pure cultures, indicating that co-culture may activate the production of bioactive compounds. From the co-culture-induced metabolites, we identified 49 putative new compounds. Taking the co-culture of <i>Streptomyces</i> sp. FXJ1.264 and <i>Mycobacterium</i> sp. HX09-1 as a case, we further explored the underlying mechanism of co-culture activation and found that it most likely relied on direct physical contact between the two living bacteria. Overall, our results verify co-culture with MACB as an effective approach to discover novel natural products from red soil-derived streptomycetes.Kairui WangNing LiuFei ShangJiao HuangBingfa YanMinghao LiuYing HuangMDPI AGarticle<i>Streptomyces</i>co-culturemycolic acid-containing bacteria (MACB)secondary metabolites (SMs)activation of natural productsBiology (General)QH301-705.5ENMicroorganisms, Vol 9, Iss 2187, p 2187 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Streptomyces</i> co-culture mycolic acid-containing bacteria (MACB) secondary metabolites (SMs) activation of natural products Biology (General) QH301-705.5
spellingShingle	<i>Streptomyces</i> co-culture mycolic acid-containing bacteria (MACB) secondary metabolites (SMs) activation of natural products Biology (General) QH301-705.5 Kairui Wang Ning Liu Fei Shang Jiao Huang Bingfa Yan Minghao Liu Ying Huang Activation of Secondary Metabolism in Red Soil-Derived Streptomycetes via Co-Culture with Mycolic Acid-Containing Bacteria
description	Our previous research has demonstrated a promising capacity of streptomycetes isolated from red soils to produce novel secondary metabolites, most of which, however, remain to be explored. Co-culturing with mycolic acid-containing bacteria (MACB) has been used successfully in activating the secondary metabolism in <i>Streptomyces</i>. Here, we co-cultured 44 strains of red soil-derived streptomycetes with four MACB of different species in a pairwise manner and analyzed the secondary metabolites. The results revealed that each of the MACB strains induced changes in the metabolite profiles of 35–40 streptomycetes tested, of which 12–14 streptomycetes produced “new” metabolites that were not detected in the pure cultures. Moreover, some of the co-cultures showed additional or enhanced antimicrobial activity compared to the pure cultures, indicating that co-culture may activate the production of bioactive compounds. From the co-culture-induced metabolites, we identified 49 putative new compounds. Taking the co-culture of <i>Streptomyces</i> sp. FXJ1.264 and <i>Mycobacterium</i> sp. HX09-1 as a case, we further explored the underlying mechanism of co-culture activation and found that it most likely relied on direct physical contact between the two living bacteria. Overall, our results verify co-culture with MACB as an effective approach to discover novel natural products from red soil-derived streptomycetes.
format	article
author	Kairui Wang Ning Liu Fei Shang Jiao Huang Bingfa Yan Minghao Liu Ying Huang
author_facet	Kairui Wang Ning Liu Fei Shang Jiao Huang Bingfa Yan Minghao Liu Ying Huang
author_sort	Kairui Wang
title	Activation of Secondary Metabolism in Red Soil-Derived Streptomycetes via Co-Culture with Mycolic Acid-Containing Bacteria
title_short	Activation of Secondary Metabolism in Red Soil-Derived Streptomycetes via Co-Culture with Mycolic Acid-Containing Bacteria
title_full	Activation of Secondary Metabolism in Red Soil-Derived Streptomycetes via Co-Culture with Mycolic Acid-Containing Bacteria
title_fullStr	Activation of Secondary Metabolism in Red Soil-Derived Streptomycetes via Co-Culture with Mycolic Acid-Containing Bacteria
title_full_unstemmed	Activation of Secondary Metabolism in Red Soil-Derived Streptomycetes via Co-Culture with Mycolic Acid-Containing Bacteria
title_sort	activation of secondary metabolism in red soil-derived streptomycetes via co-culture with mycolic acid-containing bacteria
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/db32fd85488f43d287bd24f1aac8385c
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Activation of Secondary Metabolism in Red Soil-Derived Streptomycetes via Co-Culture with Mycolic Acid-Containing Bacteria

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