INVOLVEMENT OF JNK MAPK CASCADES IN THE FORMATION OF ADHESIONS IN THE ABDOMINAL CAVITY

Adhesive process in the abdominal cavity is one of the most frequent complications of intra-abdominal surgery.The aim of the study was to evaluate the activation of the JNK MAPK cascade during the experimental adhesion process.Materials and methods. Experimental studies were performed on 40 male Wis...

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Autores principales: I. A. Shurygina, N. I. Ayushinova, L. V. Rodionova, E. E. Chepurnykh, M. G. Shurygin
Formato: article
Lenguaje:RU
Publicado: Scientific Сentre for Family Health and Human Reproduction Problems 2018
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Acceso en línea:https://doaj.org/article/db375d4fceb84938a28221c8f2d9fd5c
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Sumario:Adhesive process in the abdominal cavity is one of the most frequent complications of intra-abdominal surgery.The aim of the study was to evaluate the activation of the JNK MAPK cascade during the experimental adhesion process.Materials and methods. Experimental studies were performed on 40 male Wistar rats (9 months old). We modeled the adhesive  process in the abdominal cavity. At the time from 2 hours (2, 6, 12  hours) to 30 days (1, 3, 7, 14, 30 days), a JNK MAPK cascade was  evaluated in the zone of damage to the serosa using  immunohistochemical methods (painting on JNK1 Phospho (pT183) /  JNK2 Phospho (pT183) / JNK3 Phospho (pT221)) and real-time  PCR (the MAP Kinase Signaling Pathway RT2 - Profiler ™ PCR Array  (Qiagen) kit). Results. We have found that the expression of the  phosphorylated part of the JNK MAPK cascades has an undulating dynamics with two  peaks on the 3rd and 14th day. It has been determined that all  three JNK cascades are involved in the process, and all JNK cascades are synchronously activated on the 3rd day (JNK1 increases 12-fold  in comparison with the group of intact animals, JNK2 is 8-fold, JNK3  is 10-fold). On the 14th day, the activity of the JNK3 cascade is the  most intensively increased – 30 times (for JNK1 – about 6 times,  JNK2 – 12 times).  Conclusion. Significant activation of MARK cascades has been established on the 14th day after traumatic injury, which indicates the need for long-term prevention of posttraumatic changes, in particular, the formation of adhesions.