INVOLVEMENT OF JNK MAPK CASCADES IN THE FORMATION OF ADHESIONS IN THE ABDOMINAL CAVITY

Adhesive process in the abdominal cavity is one of the most frequent complications of intra-abdominal surgery.The aim of the study was to evaluate the activation of the JNK MAPK cascade during the experimental adhesion process.Materials and methods. Experimental studies were performed on 40 male Wis...

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Autores principales:	I. A. Shurygina, N. I. Ayushinova, L. V. Rodionova, E. E. Chepurnykh, M. G. Shurygin
Formato:	article
Lenguaje:	RU
Publicado:	Scientific Сentre for Family Health and Human Reproduction Problems 2018
Materias:	adhesive process mapk jnk modeling Science Q
Acceso en línea:	https://doaj.org/article/db375d4fceb84938a28221c8f2d9fd5c
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spelling	oai:doaj.org-article:db375d4fceb84938a28221c8f2d9fd5c2021-11-23T06:14:41ZINVOLVEMENT OF JNK MAPK CASCADES IN THE FORMATION OF ADHESIONS IN THE ABDOMINAL CAVITY2541-94202587-959610.29413/ABS.2018-3.4.18https://doaj.org/article/db375d4fceb84938a28221c8f2d9fd5c2018-07-01T00:00:00Zhttps://www.actabiomedica.ru/jour/article/view/659https://doaj.org/toc/2541-9420https://doaj.org/toc/2587-9596Adhesive process in the abdominal cavity is one of the most frequent complications of intra-abdominal surgery.The aim of the study was to evaluate the activation of the JNK MAPK cascade during the experimental adhesion process.Materials and methods. Experimental studies were performed on 40 male Wistar rats (9 months old). We modeled the adhesive process in the abdominal cavity. At the time from 2 hours (2, 6, 12 hours) to 30 days (1, 3, 7, 14, 30 days), a JNK MAPK cascade was evaluated in the zone of damage to the serosa using immunohistochemical methods (painting on JNK1 Phospho (pT183) / JNK2 Phospho (pT183) / JNK3 Phospho (pT221)) and real-time PCR (the MAP Kinase Signaling Pathway RT2 - Profiler ™ PCR Array (Qiagen) kit). Results. We have found that the expression of the phosphorylated part of the JNK MAPK cascades has an undulating dynamics with two peaks on the 3rd and 14th day. It has been determined that all three JNK cascades are involved in the process, and all JNK cascades are synchronously activated on the 3rd day (JNK1 increases 12-fold in comparison with the group of intact animals, JNK2 is 8-fold, JNK3 is 10-fold). On the 14th day, the activity of the JNK3 cascade is the most intensively increased – 30 times (for JNK1 – about 6 times, JNK2 – 12 times). Conclusion. Significant activation of MARK cascades has been established on the 14th day after traumatic injury, which indicates the need for long-term prevention of posttraumatic changes, in particular, the formation of adhesions.I. A. ShuryginaN. I. AyushinovaL. V. RodionovaE. E. ChepurnykhM. G. ShuryginScientific Сentre for Family Health and Human Reproduction Problemsarticleadhesive processmapkjnkmodelingScienceQRUActa Biomedica Scientifica, Vol 3, Iss 4, Pp 125-128 (2018)
institution	DOAJ
collection	DOAJ
language	RU
topic	adhesive process mapk jnk modeling Science Q
spellingShingle	adhesive process mapk jnk modeling Science Q I. A. Shurygina N. I. Ayushinova L. V. Rodionova E. E. Chepurnykh M. G. Shurygin INVOLVEMENT OF JNK MAPK CASCADES IN THE FORMATION OF ADHESIONS IN THE ABDOMINAL CAVITY
description	Adhesive process in the abdominal cavity is one of the most frequent complications of intra-abdominal surgery.The aim of the study was to evaluate the activation of the JNK MAPK cascade during the experimental adhesion process.Materials and methods. Experimental studies were performed on 40 male Wistar rats (9 months old). We modeled the adhesive process in the abdominal cavity. At the time from 2 hours (2, 6, 12 hours) to 30 days (1, 3, 7, 14, 30 days), a JNK MAPK cascade was evaluated in the zone of damage to the serosa using immunohistochemical methods (painting on JNK1 Phospho (pT183) / JNK2 Phospho (pT183) / JNK3 Phospho (pT221)) and real-time PCR (the MAP Kinase Signaling Pathway RT2 - Profiler ™ PCR Array (Qiagen) kit). Results. We have found that the expression of the phosphorylated part of the JNK MAPK cascades has an undulating dynamics with two peaks on the 3rd and 14th day. It has been determined that all three JNK cascades are involved in the process, and all JNK cascades are synchronously activated on the 3rd day (JNK1 increases 12-fold in comparison with the group of intact animals, JNK2 is 8-fold, JNK3 is 10-fold). On the 14th day, the activity of the JNK3 cascade is the most intensively increased – 30 times (for JNK1 – about 6 times, JNK2 – 12 times). Conclusion. Significant activation of MARK cascades has been established on the 14th day after traumatic injury, which indicates the need for long-term prevention of posttraumatic changes, in particular, the formation of adhesions.
format	article
author	I. A. Shurygina N. I. Ayushinova L. V. Rodionova E. E. Chepurnykh M. G. Shurygin
author_facet	I. A. Shurygina N. I. Ayushinova L. V. Rodionova E. E. Chepurnykh M. G. Shurygin
author_sort	I. A. Shurygina
title	INVOLVEMENT OF JNK MAPK CASCADES IN THE FORMATION OF ADHESIONS IN THE ABDOMINAL CAVITY
title_short	INVOLVEMENT OF JNK MAPK CASCADES IN THE FORMATION OF ADHESIONS IN THE ABDOMINAL CAVITY
title_full	INVOLVEMENT OF JNK MAPK CASCADES IN THE FORMATION OF ADHESIONS IN THE ABDOMINAL CAVITY
title_fullStr	INVOLVEMENT OF JNK MAPK CASCADES IN THE FORMATION OF ADHESIONS IN THE ABDOMINAL CAVITY
title_full_unstemmed	INVOLVEMENT OF JNK MAPK CASCADES IN THE FORMATION OF ADHESIONS IN THE ABDOMINAL CAVITY
title_sort	involvement of jnk mapk cascades in the formation of adhesions in the abdominal cavity
publisher	Scientific Сentre for Family Health and Human Reproduction Problems
publishDate	2018
url	https://doaj.org/article/db375d4fceb84938a28221c8f2d9fd5c
work_keys_str_mv	AT iashurygina involvementofjnkmapkcascadesintheformationofadhesionsintheabdominalcavity AT niayushinova involvementofjnkmapkcascadesintheformationofadhesionsintheabdominalcavity AT lvrodionova involvementofjnkmapkcascadesintheformationofadhesionsintheabdominalcavity AT eechepurnykh involvementofjnkmapkcascadesintheformationofadhesionsintheabdominalcavity AT mgshurygin involvementofjnkmapkcascadesintheformationofadhesionsintheabdominalcavity
_version_	1718416978550456320

INVOLVEMENT OF JNK MAPK CASCADES IN THE FORMATION OF ADHESIONS IN THE ABDOMINAL CAVITY

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