Comparative Analysis of Blood and Marrow Cellular Structure of White Mice Infected With Bacillus Anthracis Different Genotypes

Background. Detection of the pathogenicity factors is one of the actual directions in studying of the anthrax causative agent. First of all, it includes the presence of tripartite toxin, capsule and the related structural and regulatory genes. Their presence or absence is defined by structural chang...

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Autores principales: V. I. Dubrovina, T. P. Starovoitova, E. V. Kravets, T. A. Ivanova, Z. F. Dugarzhapova, O. V. Gavrilova, O. V. Yur’eva, T. T. Shkaruba, S. V. Balakhonov
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Publicado: Scientific Сentre for Family Health and Human Reproduction Problems 2020
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spelling oai:doaj.org-article:db37ff5a466041c1a7894a39f23327952021-11-23T06:14:43ZComparative Analysis of Blood and Marrow Cellular Structure of White Mice Infected With Bacillus Anthracis Different Genotypes2541-94202587-959610.29413/ABS.2020-5.1.10https://doaj.org/article/db37ff5a466041c1a7894a39f23327952020-03-01T00:00:00Zhttps://www.actabiomedica.ru/jour/article/view/2242https://doaj.org/toc/2541-9420https://doaj.org/toc/2587-9596Background. Detection of the pathogenicity factors is one of the actual directions in studying of the anthrax causative agent. First of all, it includes the presence of tripartite toxin, capsule and the related structural and regulatory genes. Their presence or absence is defined by structural changes in cellular structure of blood and immunocomplex organs of a macroorganism.Objective: To conduct a comparative analysis of subpopulational structure of marrow and peripheral blood cells at anthrax infection in experimental animals and to trace dynamics of the infectious process caused by Bacillus anthracis of different genotypes.Methods. Comparative examination of subpopulational structure of red marrow and peripheral blood cells of white mice in dynamics of the infectious process caused by B. anthracis of different genotypes was conducted. Also, neutrophil maturing index and leucoerythrocytic correlation were calculated in red marrow. Degree of manifestation of neutrophil pathological granularity was revealed in peripheral blood smears stained by the Freifeld’s method. Statistical processing of the received data was conducted using the computer program “Statistics”, version 6 (Novosibirsk). Authentic results were considered if the error probability was less than 0.05 (р < 0.05) in relation to the control.Results. It is revealed that alterations in white mice infected by B. anthracis strains with the same set of plasmids are significantly various.Conclusion. The resulted data permit to assume that the distinction of pathological alterations in peripheral blood and red marrow is probably connected with the other factors of the pathogen invasiveness like enzymatic activity, ability to adhesion and many others.V. I. DubrovinaT. P. StarovoitovaE. V. KravetsT. A. IvanovaZ. F. DugarzhapovaO. V. GavrilovaO. V. Yur’evaT. T. ShkarubaS. V. BalakhonovScientific Сentre for Family Health and Human Reproduction Problemsarticlebacillus anthracisbloodmarrowplasmid profileScienceQRUActa Biomedica Scientifica, Vol 5, Iss 1, Pp 72-77 (2020)
institution DOAJ
collection DOAJ
language RU
topic bacillus anthracis
blood
marrow
plasmid profile
Science
Q
spellingShingle bacillus anthracis
blood
marrow
plasmid profile
Science
Q
V. I. Dubrovina
T. P. Starovoitova
E. V. Kravets
T. A. Ivanova
Z. F. Dugarzhapova
O. V. Gavrilova
O. V. Yur’eva
T. T. Shkaruba
S. V. Balakhonov
Comparative Analysis of Blood and Marrow Cellular Structure of White Mice Infected With Bacillus Anthracis Different Genotypes
description Background. Detection of the pathogenicity factors is one of the actual directions in studying of the anthrax causative agent. First of all, it includes the presence of tripartite toxin, capsule and the related structural and regulatory genes. Their presence or absence is defined by structural changes in cellular structure of blood and immunocomplex organs of a macroorganism.Objective: To conduct a comparative analysis of subpopulational structure of marrow and peripheral blood cells at anthrax infection in experimental animals and to trace dynamics of the infectious process caused by Bacillus anthracis of different genotypes.Methods. Comparative examination of subpopulational structure of red marrow and peripheral blood cells of white mice in dynamics of the infectious process caused by B. anthracis of different genotypes was conducted. Also, neutrophil maturing index and leucoerythrocytic correlation were calculated in red marrow. Degree of manifestation of neutrophil pathological granularity was revealed in peripheral blood smears stained by the Freifeld’s method. Statistical processing of the received data was conducted using the computer program “Statistics”, version 6 (Novosibirsk). Authentic results were considered if the error probability was less than 0.05 (р < 0.05) in relation to the control.Results. It is revealed that alterations in white mice infected by B. anthracis strains with the same set of plasmids are significantly various.Conclusion. The resulted data permit to assume that the distinction of pathological alterations in peripheral blood and red marrow is probably connected with the other factors of the pathogen invasiveness like enzymatic activity, ability to adhesion and many others.
format article
author V. I. Dubrovina
T. P. Starovoitova
E. V. Kravets
T. A. Ivanova
Z. F. Dugarzhapova
O. V. Gavrilova
O. V. Yur’eva
T. T. Shkaruba
S. V. Balakhonov
author_facet V. I. Dubrovina
T. P. Starovoitova
E. V. Kravets
T. A. Ivanova
Z. F. Dugarzhapova
O. V. Gavrilova
O. V. Yur’eva
T. T. Shkaruba
S. V. Balakhonov
author_sort V. I. Dubrovina
title Comparative Analysis of Blood and Marrow Cellular Structure of White Mice Infected With Bacillus Anthracis Different Genotypes
title_short Comparative Analysis of Blood and Marrow Cellular Structure of White Mice Infected With Bacillus Anthracis Different Genotypes
title_full Comparative Analysis of Blood and Marrow Cellular Structure of White Mice Infected With Bacillus Anthracis Different Genotypes
title_fullStr Comparative Analysis of Blood and Marrow Cellular Structure of White Mice Infected With Bacillus Anthracis Different Genotypes
title_full_unstemmed Comparative Analysis of Blood and Marrow Cellular Structure of White Mice Infected With Bacillus Anthracis Different Genotypes
title_sort comparative analysis of blood and marrow cellular structure of white mice infected with bacillus anthracis different genotypes
publisher Scientific Сentre for Family Health and Human Reproduction Problems
publishDate 2020
url https://doaj.org/article/db37ff5a466041c1a7894a39f2332795
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