miR-155, miR-21, and let-7a Expressions in MCF-10A and MCF-7 Cell Lines after Low to High Dose Irradiation

Objective: Ionizing radiation is a tremendous risk factor for cancer development. MicroRNAs (miRNAs) are regulators that utilize cell pathways, which are implicated in human cancer prognosis. In addition, miRNAs respond to anti-cancer therapy and proliferation after irradiation. However, the changes...

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Autores principales:	Afsaneh Zare, Reza Fardid, Gholam Hossein Tamadon, Mohammad Amin Mosleh-Shirazi
Formato:	article
Lenguaje:	EN
Publicado:	Royan Institute (ACECR), Tehran 2021
Materias:	breast cancer ionizing radiation let-7a mir-155 mir-21 Medicine R Science Q
Acceso en línea:	https://doaj.org/article/db3d552637434aaf8af30414edd2e9aa
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spelling	oai:doaj.org-article:db3d552637434aaf8af30414edd2e9aa2021-11-07T07:16:25ZmiR-155, miR-21, and let-7a Expressions in MCF-10A and MCF-7 Cell Lines after Low to High Dose Irradiation10.22074/cellj.2021.74112228-58062228-5814https://doaj.org/article/db3d552637434aaf8af30414edd2e9aa2021-10-01T00:00:00Zhttps://celljournal.org/journal/article/fulltext/the-expression-of-mir-155-mir-21-let-7a-in-mcf-10a-and-mcf-7-cell-lines-after-low-dose-to-high-dose-irradiation.pdfhttps://doaj.org/toc/2228-5806https://doaj.org/toc/2228-5814Objective: Ionizing radiation is a tremendous risk factor for cancer development. MicroRNAs (miRNAs) are regulators that utilize cell pathways, which are implicated in human cancer prognosis. In addition, miRNAs respond to anti-cancer therapy and proliferation after irradiation. However, the changes in miRNA expression profiles in response to irradiation have not been comprehensively analysed. The present study was designed to assess potential changes that occur in miRNA expression following irradiation. Materials and Methods: In this experimental study, we used quantitative real-time polymerase chain reaction (qRTPCR) to measure the expressions of miR-155, miR-21, and let-7a in MCF-10A (normal breast cells) and MCF-7 (breast cancer cells) six hours after the cells were exposed to five different irradiation doses (50, 100, 400, 2000, and 4000 mGY). Results: After irradiation from the low to high doses, we observed an upsurge in miR-155 (more than 100%) expression and reduction in let-7a (more than 87%) expression. However, there was an increase and a reduction in miR-21 expression (more than 100%). Conclusion: Irradiation can play an important role in cancer development in normal breast cells (MCF-10A) at low dose irradiation. However, the results showed little difference at high doses of radiation.Afsaneh ZareReza FardidGholam Hossein TamadonMohammad Amin Mosleh-ShiraziRoyan Institute (ACECR), Tehranarticlebreast cancerionizing radiationlet-7amir-155mir-21MedicineRScienceQENCell Journal, Vol 23, Iss 5, Pp 532-537 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	breast cancer ionizing radiation let-7a mir-155 mir-21 Medicine R Science Q
spellingShingle	breast cancer ionizing radiation let-7a mir-155 mir-21 Medicine R Science Q Afsaneh Zare Reza Fardid Gholam Hossein Tamadon Mohammad Amin Mosleh-Shirazi miR-155, miR-21, and let-7a Expressions in MCF-10A and MCF-7 Cell Lines after Low to High Dose Irradiation
description	Objective: Ionizing radiation is a tremendous risk factor for cancer development. MicroRNAs (miRNAs) are regulators that utilize cell pathways, which are implicated in human cancer prognosis. In addition, miRNAs respond to anti-cancer therapy and proliferation after irradiation. However, the changes in miRNA expression profiles in response to irradiation have not been comprehensively analysed. The present study was designed to assess potential changes that occur in miRNA expression following irradiation. Materials and Methods: In this experimental study, we used quantitative real-time polymerase chain reaction (qRTPCR) to measure the expressions of miR-155, miR-21, and let-7a in MCF-10A (normal breast cells) and MCF-7 (breast cancer cells) six hours after the cells were exposed to five different irradiation doses (50, 100, 400, 2000, and 4000 mGY). Results: After irradiation from the low to high doses, we observed an upsurge in miR-155 (more than 100%) expression and reduction in let-7a (more than 87%) expression. However, there was an increase and a reduction in miR-21 expression (more than 100%). Conclusion: Irradiation can play an important role in cancer development in normal breast cells (MCF-10A) at low dose irradiation. However, the results showed little difference at high doses of radiation.
format	article
author	Afsaneh Zare Reza Fardid Gholam Hossein Tamadon Mohammad Amin Mosleh-Shirazi
author_facet	Afsaneh Zare Reza Fardid Gholam Hossein Tamadon Mohammad Amin Mosleh-Shirazi
author_sort	Afsaneh Zare
title	miR-155, miR-21, and let-7a Expressions in MCF-10A and MCF-7 Cell Lines after Low to High Dose Irradiation
title_short	miR-155, miR-21, and let-7a Expressions in MCF-10A and MCF-7 Cell Lines after Low to High Dose Irradiation
title_full	miR-155, miR-21, and let-7a Expressions in MCF-10A and MCF-7 Cell Lines after Low to High Dose Irradiation
title_fullStr	miR-155, miR-21, and let-7a Expressions in MCF-10A and MCF-7 Cell Lines after Low to High Dose Irradiation
title_full_unstemmed	miR-155, miR-21, and let-7a Expressions in MCF-10A and MCF-7 Cell Lines after Low to High Dose Irradiation
title_sort	mir-155, mir-21, and let-7a expressions in mcf-10a and mcf-7 cell lines after low to high dose irradiation
publisher	Royan Institute (ACECR), Tehran
publishDate	2021
url	https://doaj.org/article/db3d552637434aaf8af30414edd2e9aa
work_keys_str_mv	AT afsanehzare mir155mir21andlet7aexpressionsinmcf10aandmcf7celllinesafterlowtohighdoseirradiation AT rezafardid mir155mir21andlet7aexpressionsinmcf10aandmcf7celllinesafterlowtohighdoseirradiation AT gholamhosseintamadon mir155mir21andlet7aexpressionsinmcf10aandmcf7celllinesafterlowtohighdoseirradiation AT mohammadaminmoslehshirazi mir155mir21andlet7aexpressionsinmcf10aandmcf7celllinesafterlowtohighdoseirradiation
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miR-155, miR-21, and let-7a Expressions in MCF-10A and MCF-7 Cell Lines after Low to High Dose Irradiation

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