Chemical and thermal stabilization of CotA laccase via a novel one-step expression and immobilization in muNS-Mi nanospheres

Abstract A methodology that programs eukaryotic or bacterial cells to encapsulate proteins of any kind inside micro/nanospheres formed by muNS-Mi viral protein was developed in our laboratory. In the present study such “in cellulo” encapsulation technology is utilized for immobilizing a protein with...

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Autores principales:	Tomás Pose-Boirazian, Gemma Eibes, Natalia Barreiro-Piñeiro, Cristina Díaz-Jullien, Juan M. Lema, Jose Martínez-Costas
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/db401298f7524f42811d4851e41dbe1e
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spelling	oai:doaj.org-article:db401298f7524f42811d4851e41dbe1e2021-12-02T10:44:21ZChemical and thermal stabilization of CotA laccase via a novel one-step expression and immobilization in muNS-Mi nanospheres10.1038/s41598-021-82468-x2045-2322https://doaj.org/article/db401298f7524f42811d4851e41dbe1e2021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-82468-xhttps://doaj.org/toc/2045-2322Abstract A methodology that programs eukaryotic or bacterial cells to encapsulate proteins of any kind inside micro/nanospheres formed by muNS-Mi viral protein was developed in our laboratory. In the present study such “in cellulo” encapsulation technology is utilized for immobilizing a protein with an enzymatic activity of industrial interest, CotA laccase. The encapsulation facilitates its purification, resulting in a cost-effective, one-step way of producing immobilized enzymes for industrial use. In addition to the ability to be recycled without activity loss, the encapsulated protein showed an increased pH working range and high resistance to chemical inactivation. Also, its activity was almost unaffected after 30 min incubation at 90 °C and 15 min at the almost-boiling temperature of 95 °C. Furthermore, the encapsulated laccase was able to efficiently decolorate the recalcitrant dye RB19 at room temperature.Tomás Pose-BoirazianGemma EibesNatalia Barreiro-PiñeiroCristina Díaz-JullienJuan M. LemaJose Martínez-CostasNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tomás Pose-Boirazian Gemma Eibes Natalia Barreiro-Piñeiro Cristina Díaz-Jullien Juan M. Lema Jose Martínez-Costas Chemical and thermal stabilization of CotA laccase via a novel one-step expression and immobilization in muNS-Mi nanospheres
description	Abstract A methodology that programs eukaryotic or bacterial cells to encapsulate proteins of any kind inside micro/nanospheres formed by muNS-Mi viral protein was developed in our laboratory. In the present study such “in cellulo” encapsulation technology is utilized for immobilizing a protein with an enzymatic activity of industrial interest, CotA laccase. The encapsulation facilitates its purification, resulting in a cost-effective, one-step way of producing immobilized enzymes for industrial use. In addition to the ability to be recycled without activity loss, the encapsulated protein showed an increased pH working range and high resistance to chemical inactivation. Also, its activity was almost unaffected after 30 min incubation at 90 °C and 15 min at the almost-boiling temperature of 95 °C. Furthermore, the encapsulated laccase was able to efficiently decolorate the recalcitrant dye RB19 at room temperature.
format	article
author	Tomás Pose-Boirazian Gemma Eibes Natalia Barreiro-Piñeiro Cristina Díaz-Jullien Juan M. Lema Jose Martínez-Costas
author_facet	Tomás Pose-Boirazian Gemma Eibes Natalia Barreiro-Piñeiro Cristina Díaz-Jullien Juan M. Lema Jose Martínez-Costas
author_sort	Tomás Pose-Boirazian
title	Chemical and thermal stabilization of CotA laccase via a novel one-step expression and immobilization in muNS-Mi nanospheres
title_short	Chemical and thermal stabilization of CotA laccase via a novel one-step expression and immobilization in muNS-Mi nanospheres
title_full	Chemical and thermal stabilization of CotA laccase via a novel one-step expression and immobilization in muNS-Mi nanospheres
title_fullStr	Chemical and thermal stabilization of CotA laccase via a novel one-step expression and immobilization in muNS-Mi nanospheres
title_full_unstemmed	Chemical and thermal stabilization of CotA laccase via a novel one-step expression and immobilization in muNS-Mi nanospheres
title_sort	chemical and thermal stabilization of cota laccase via a novel one-step expression and immobilization in muns-mi nanospheres
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/db401298f7524f42811d4851e41dbe1e
work_keys_str_mv	AT tomasposeboirazian chemicalandthermalstabilizationofcotalaccaseviaanovelonestepexpressionandimmobilizationinmunsminanospheres AT gemmaeibes chemicalandthermalstabilizationofcotalaccaseviaanovelonestepexpressionandimmobilizationinmunsminanospheres AT nataliabarreiropineiro chemicalandthermalstabilizationofcotalaccaseviaanovelonestepexpressionandimmobilizationinmunsminanospheres AT cristinadiazjullien chemicalandthermalstabilizationofcotalaccaseviaanovelonestepexpressionandimmobilizationinmunsminanospheres AT juanmlema chemicalandthermalstabilizationofcotalaccaseviaanovelonestepexpressionandimmobilizationinmunsminanospheres AT josemartinezcostas chemicalandthermalstabilizationofcotalaccaseviaanovelonestepexpressionandimmobilizationinmunsminanospheres
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Chemical and thermal stabilization of CotA laccase via a novel one-step expression and immobilization in muNS-Mi nanospheres

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