Pullulan/dextran/nHA macroporous composite beads for bone repair in a femoral condyle defect in rats.

The repair of bone defects is of particular interest for orthopedic, oral, maxillofacial, and dental surgery. Bone loss requiring reconstruction is conventionally addressed through bone grafting. Depending on the size and the location of the defect, this method has limits and risks. Biomaterials can...

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Autores principales:	Silke Schlaubitz, Sidi Mohammed Derkaoui, Lydia Marosa, Sylvain Miraux, Martine Renard, Sylvain Catros, Catherine Le Visage, Didier Letourneur, Joëlle Amédée, Jean-Christophe Fricain
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/db482bfedd254481a33fbaf6e1be5cb3
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spelling	oai:doaj.org-article:db482bfedd254481a33fbaf6e1be5cb32021-11-25T05:55:54ZPullulan/dextran/nHA macroporous composite beads for bone repair in a femoral condyle defect in rats.1932-620310.1371/journal.pone.0110251https://doaj.org/article/db482bfedd254481a33fbaf6e1be5cb32014-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0110251https://doaj.org/toc/1932-6203The repair of bone defects is of particular interest for orthopedic, oral, maxillofacial, and dental surgery. Bone loss requiring reconstruction is conventionally addressed through bone grafting. Depending on the size and the location of the defect, this method has limits and risks. Biomaterials can offer an alternative and have features supporting bone repair. Here, we propose to evaluate the cellular penetration and bone formation of new macroporous beads based on pullulan/dextran that has been supplemented with nanocrystalline hydroxyapatite in a rat model. Cross-linked beads of 300-500 µm diameters were used in a lateral femoral condyle defect and analyzed by magnetic resonance imaging, micro-computed tomography, and histology in comparison to the empty defects 15, 30, and 70 days after implantation. Inflammation was absent for both conditions. For empty defects, cellularisation and mineralization started from the periphery of the defect. For the defects containing beads, cellular structures filling out the spaces between the scaffolds with increasing interconnectivity and trabecular-like organization were observed over time. The analysis of calcified sections showed increased mineralization over time for both conditions, but was more pronounced for the samples containing beads. Bone Mineral Density and Bone Mineral Content were both significantly higher at day 70 for the beads in comparison to empty defects as well as compared with earlier time points. Analysis of newly formed tissue around the beads showed an increase of osteoid tissue, measured as percentage of the defect surface. This study suggests that the use of beads for the repair of small size defects in bone may be expanded on to meet the clinical need for a ready-to-use fill-up material that can favor bone formation and mineralization, as well as promote vessel ingrowth into the defect site.Silke SchlaubitzSidi Mohammed DerkaouiLydia MarosaSylvain MirauxMartine RenardSylvain CatrosCatherine Le VisageDidier LetourneurJoëlle AmédéeJean-Christophe FricainPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 10, p e110251 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Silke Schlaubitz Sidi Mohammed Derkaoui Lydia Marosa Sylvain Miraux Martine Renard Sylvain Catros Catherine Le Visage Didier Letourneur Joëlle Amédée Jean-Christophe Fricain Pullulan/dextran/nHA macroporous composite beads for bone repair in a femoral condyle defect in rats.
description	The repair of bone defects is of particular interest for orthopedic, oral, maxillofacial, and dental surgery. Bone loss requiring reconstruction is conventionally addressed through bone grafting. Depending on the size and the location of the defect, this method has limits and risks. Biomaterials can offer an alternative and have features supporting bone repair. Here, we propose to evaluate the cellular penetration and bone formation of new macroporous beads based on pullulan/dextran that has been supplemented with nanocrystalline hydroxyapatite in a rat model. Cross-linked beads of 300-500 µm diameters were used in a lateral femoral condyle defect and analyzed by magnetic resonance imaging, micro-computed tomography, and histology in comparison to the empty defects 15, 30, and 70 days after implantation. Inflammation was absent for both conditions. For empty defects, cellularisation and mineralization started from the periphery of the defect. For the defects containing beads, cellular structures filling out the spaces between the scaffolds with increasing interconnectivity and trabecular-like organization were observed over time. The analysis of calcified sections showed increased mineralization over time for both conditions, but was more pronounced for the samples containing beads. Bone Mineral Density and Bone Mineral Content were both significantly higher at day 70 for the beads in comparison to empty defects as well as compared with earlier time points. Analysis of newly formed tissue around the beads showed an increase of osteoid tissue, measured as percentage of the defect surface. This study suggests that the use of beads for the repair of small size defects in bone may be expanded on to meet the clinical need for a ready-to-use fill-up material that can favor bone formation and mineralization, as well as promote vessel ingrowth into the defect site.
format	article
author	Silke Schlaubitz Sidi Mohammed Derkaoui Lydia Marosa Sylvain Miraux Martine Renard Sylvain Catros Catherine Le Visage Didier Letourneur Joëlle Amédée Jean-Christophe Fricain
author_facet	Silke Schlaubitz Sidi Mohammed Derkaoui Lydia Marosa Sylvain Miraux Martine Renard Sylvain Catros Catherine Le Visage Didier Letourneur Joëlle Amédée Jean-Christophe Fricain
author_sort	Silke Schlaubitz
title	Pullulan/dextran/nHA macroporous composite beads for bone repair in a femoral condyle defect in rats.
title_short	Pullulan/dextran/nHA macroporous composite beads for bone repair in a femoral condyle defect in rats.
title_full	Pullulan/dextran/nHA macroporous composite beads for bone repair in a femoral condyle defect in rats.
title_fullStr	Pullulan/dextran/nHA macroporous composite beads for bone repair in a femoral condyle defect in rats.
title_full_unstemmed	Pullulan/dextran/nHA macroporous composite beads for bone repair in a femoral condyle defect in rats.
title_sort	pullulan/dextran/nha macroporous composite beads for bone repair in a femoral condyle defect in rats.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/db482bfedd254481a33fbaf6e1be5cb3
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Pullulan/dextran/nHA macroporous composite beads for bone repair in a femoral condyle defect in rats.

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