Development of an immunochromatographic strip for the rapid detection of maduramicin in chicken and egg samples

Following the steps of antigen synthesis, immunization, cell fusion, ascites preparation and purification, a maduramicin (MD) monoclonal antibody (mAb) was produced. This MD-mAb demonstrated a 50% inhibition concentration value of 3.75 ng/ml, an affinity constant of 3.70 × 1010 l/mol, and the isoty...

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Autores principales:	Lingling Guo, Liguang Xu, Shanshan Song, Liqiang Liu, Hua Kuang
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2018
Materias:	maduramicin strip test chicken breast egg Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/db4ba3b383fd4b099ce65159906e42d4
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spelling	oai:doaj.org-article:db4ba3b383fd4b099ce65159906e42d42021-11-26T11:19:46ZDevelopment of an immunochromatographic strip for the rapid detection of maduramicin in chicken and egg samples0954-01051465-344310.1080/09540105.2017.1401045https://doaj.org/article/db4ba3b383fd4b099ce65159906e42d42018-01-01T00:00:00Zhttp://dx.doi.org/10.1080/09540105.2017.1401045https://doaj.org/toc/0954-0105https://doaj.org/toc/1465-3443Following the steps of antigen synthesis, immunization, cell fusion, ascites preparation and purification, a maduramicin (MD) monoclonal antibody (mAb) was produced. This MD-mAb demonstrated a 50% inhibition concentration value of 3.75 ng/ml, an affinity constant of 3.70 × 1010 l/mol, and the isotype was IgG3. The MD-mAb has no cross-reactivity with other polyether antibiotics. Using this MD-mAb, a gold immunochromatographic assay was developed to detect MD residues in chicken breast and egg samples. For semi-quantitative detection by the naked eye, the visual limit of detection was 5 ng/g in chicken breast, 10 ng/g in egg. Quantitative results can be obtained by a hand-held strip scan reader, with the detection range of 5.11–19.34 ng/g in chicken breast and 6.46–27.87 ng/g in egg. The strip test took 10 min to run in total. This strip assay is suitable for on-site detection of MD residues in chicken breast and egg samples.Lingling GuoLiguang XuShanshan SongLiqiang LiuHua KuangTaylor & Francis Grouparticlemaduramicinstrip testchicken breasteggAgriculture (General)S1-972Immunologic diseases. AllergyRC581-607ENFood and Agricultural Immunology, Vol 29, Iss 1, Pp 458-469 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	maduramicin strip test chicken breast egg Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607
spellingShingle	maduramicin strip test chicken breast egg Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607 Lingling Guo Liguang Xu Shanshan Song Liqiang Liu Hua Kuang Development of an immunochromatographic strip for the rapid detection of maduramicin in chicken and egg samples
description	Following the steps of antigen synthesis, immunization, cell fusion, ascites preparation and purification, a maduramicin (MD) monoclonal antibody (mAb) was produced. This MD-mAb demonstrated a 50% inhibition concentration value of 3.75 ng/ml, an affinity constant of 3.70 × 1010 l/mol, and the isotype was IgG3. The MD-mAb has no cross-reactivity with other polyether antibiotics. Using this MD-mAb, a gold immunochromatographic assay was developed to detect MD residues in chicken breast and egg samples. For semi-quantitative detection by the naked eye, the visual limit of detection was 5 ng/g in chicken breast, 10 ng/g in egg. Quantitative results can be obtained by a hand-held strip scan reader, with the detection range of 5.11–19.34 ng/g in chicken breast and 6.46–27.87 ng/g in egg. The strip test took 10 min to run in total. This strip assay is suitable for on-site detection of MD residues in chicken breast and egg samples.
format	article
author	Lingling Guo Liguang Xu Shanshan Song Liqiang Liu Hua Kuang
author_facet	Lingling Guo Liguang Xu Shanshan Song Liqiang Liu Hua Kuang
author_sort	Lingling Guo
title	Development of an immunochromatographic strip for the rapid detection of maduramicin in chicken and egg samples
title_short	Development of an immunochromatographic strip for the rapid detection of maduramicin in chicken and egg samples
title_full	Development of an immunochromatographic strip for the rapid detection of maduramicin in chicken and egg samples
title_fullStr	Development of an immunochromatographic strip for the rapid detection of maduramicin in chicken and egg samples
title_full_unstemmed	Development of an immunochromatographic strip for the rapid detection of maduramicin in chicken and egg samples
title_sort	development of an immunochromatographic strip for the rapid detection of maduramicin in chicken and egg samples
publisher	Taylor & Francis Group
publishDate	2018
url	https://doaj.org/article/db4ba3b383fd4b099ce65159906e42d4
work_keys_str_mv	AT linglingguo developmentofanimmunochromatographicstripfortherapiddetectionofmaduramicininchickenandeggsamples AT liguangxu developmentofanimmunochromatographicstripfortherapiddetectionofmaduramicininchickenandeggsamples AT shanshansong developmentofanimmunochromatographicstripfortherapiddetectionofmaduramicininchickenandeggsamples AT liqiangliu developmentofanimmunochromatographicstripfortherapiddetectionofmaduramicininchickenandeggsamples AT huakuang developmentofanimmunochromatographicstripfortherapiddetectionofmaduramicininchickenandeggsamples
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Development of an immunochromatographic strip for the rapid detection of maduramicin in chicken and egg samples

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