Insect derived extra oral GH32 plays a role in susceptibility of wheat to Hessian fly

Abstract The Hessian fly is an obligate parasite of wheat causing significant economic damage, and triggers either a resistant or susceptible reaction. However, the molecular mechanisms of susceptibility leading to the establishment of the larvae are unknown. Larval survival on the plant requires th...

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Autores principales:	Subhashree Subramanyam, Jill A. Nemacheck, Victor Bernal-Crespo, Nagesh Sardesai
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/db4c4dac026a4f538ed5bef21e950c6d
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spelling	oai:doaj.org-article:db4c4dac026a4f538ed5bef21e950c6d2021-12-02T13:51:06ZInsect derived extra oral GH32 plays a role in susceptibility of wheat to Hessian fly10.1038/s41598-021-81481-42045-2322https://doaj.org/article/db4c4dac026a4f538ed5bef21e950c6d2021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-81481-4https://doaj.org/toc/2045-2322Abstract The Hessian fly is an obligate parasite of wheat causing significant economic damage, and triggers either a resistant or susceptible reaction. However, the molecular mechanisms of susceptibility leading to the establishment of the larvae are unknown. Larval survival on the plant requires the establishment of a steady source of readily available nutrition. Unlike other insect pests, the Hessian fly larvae have minute mandibles and cannot derive their nutrition by chewing tissue or sucking phloem sap. Here, we show that the virulent larvae produce the glycoside hydrolase MdesGH32 extra-orally, that localizes within the leaf tissue being fed upon. MdesGH32 has strong inulinase and invertase activity aiding in the breakdown of the plant cell wall inulin polymer into monomers and converting sucrose, the primary transport sugar in plants, to glucose and fructose, resulting in the formation of a nutrient-rich tissue. Our finding elucidates the molecular mechanism of nutrient sink formation and establishment of susceptibility.Subhashree SubramanyamJill A. NemacheckVictor Bernal-CrespoNagesh SardesaiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Subhashree Subramanyam Jill A. Nemacheck Victor Bernal-Crespo Nagesh Sardesai Insect derived extra oral GH32 plays a role in susceptibility of wheat to Hessian fly
description	Abstract The Hessian fly is an obligate parasite of wheat causing significant economic damage, and triggers either a resistant or susceptible reaction. However, the molecular mechanisms of susceptibility leading to the establishment of the larvae are unknown. Larval survival on the plant requires the establishment of a steady source of readily available nutrition. Unlike other insect pests, the Hessian fly larvae have minute mandibles and cannot derive their nutrition by chewing tissue or sucking phloem sap. Here, we show that the virulent larvae produce the glycoside hydrolase MdesGH32 extra-orally, that localizes within the leaf tissue being fed upon. MdesGH32 has strong inulinase and invertase activity aiding in the breakdown of the plant cell wall inulin polymer into monomers and converting sucrose, the primary transport sugar in plants, to glucose and fructose, resulting in the formation of a nutrient-rich tissue. Our finding elucidates the molecular mechanism of nutrient sink formation and establishment of susceptibility.
format	article
author	Subhashree Subramanyam Jill A. Nemacheck Victor Bernal-Crespo Nagesh Sardesai
author_facet	Subhashree Subramanyam Jill A. Nemacheck Victor Bernal-Crespo Nagesh Sardesai
author_sort	Subhashree Subramanyam
title	Insect derived extra oral GH32 plays a role in susceptibility of wheat to Hessian fly
title_short	Insect derived extra oral GH32 plays a role in susceptibility of wheat to Hessian fly
title_full	Insect derived extra oral GH32 plays a role in susceptibility of wheat to Hessian fly
title_fullStr	Insect derived extra oral GH32 plays a role in susceptibility of wheat to Hessian fly
title_full_unstemmed	Insect derived extra oral GH32 plays a role in susceptibility of wheat to Hessian fly
title_sort	insect derived extra oral gh32 plays a role in susceptibility of wheat to hessian fly
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/db4c4dac026a4f538ed5bef21e950c6d
work_keys_str_mv	AT subhashreesubramanyam insectderivedextraoralgh32playsaroleinsusceptibilityofwheattohessianfly AT jillanemacheck insectderivedextraoralgh32playsaroleinsusceptibilityofwheattohessianfly AT victorbernalcrespo insectderivedextraoralgh32playsaroleinsusceptibilityofwheattohessianfly AT nageshsardesai insectderivedextraoralgh32playsaroleinsusceptibilityofwheattohessianfly
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Insect derived extra oral GH32 plays a role in susceptibility of wheat to Hessian fly

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