Transcriptome profiling in susceptible and tolerant rubber tree clones in response to cassiicolin Cas1, a necrotrophic effector from Corynespora cassiicola.

Corynespora cassiicola, a fungal plant pathogen with a large host range, causes important damages in rubber tree (Hevea brasiliensis), in Asia and Africa. A small secreted protein named cassiicolin was previously identified as a necrotrophic effector required for the virulence of C. cassiicola in sp...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Sébastien Ribeiro, Philippe Label, Dominique Garcia, Pascal Montoro, Valérie Pujade-Renaud
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/db4e5e980f584ed983d48f69ec32ccb2
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:db4e5e980f584ed983d48f69ec32ccb2
record_format	dspace
spelling	oai:doaj.org-article:db4e5e980f584ed983d48f69ec32ccb22021-12-02T20:09:04ZTranscriptome profiling in susceptible and tolerant rubber tree clones in response to cassiicolin Cas1, a necrotrophic effector from Corynespora cassiicola.1932-620310.1371/journal.pone.0254541https://doaj.org/article/db4e5e980f584ed983d48f69ec32ccb22021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0254541https://doaj.org/toc/1932-6203Corynespora cassiicola, a fungal plant pathogen with a large host range, causes important damages in rubber tree (Hevea brasiliensis), in Asia and Africa. A small secreted protein named cassiicolin was previously identified as a necrotrophic effector required for the virulence of C. cassiicola in specific rubber tree clones. The objective of this study was to decipher the cassiicolin-mediated molecular mechanisms involved in this compatible interaction. We comparatively analyzed the RNA-Seq transcriptomic profiles of leaves treated or not with the purified cassiicolin Cas1, in two rubber clones: PB260 (susceptible) and RRIM600 (tolerant). The reads were mapped against a synthetic transcriptome composed of all available transcriptomic references from the two clones. Genes differentially expressed in response to cassiicolin Cas1 were identified, in each clone, at two different time-points. After de novo annotation of the synthetic transcriptome, we analyzed GO enrichment of the differentially expressed genes in order to elucidate the main functional pathways impacted by cassiicolin. Cassiicolin induced qualitatively similar transcriptional modifications in both the susceptible and the tolerant clones, with a strong negative impact on photosynthesis, and the activation of defense responses via redox signaling, production of pathogenesis-related protein, or activation of the secondary metabolism. In the tolerant clone, transcriptional reprogramming occurred earlier but remained moderate. By contrast, the susceptible clone displayed a late but huge transcriptional burst, characterized by massive induction of phosphorylation events and all the features of a hypersensitive response. These results confirm that cassiicolin Cas1 is a necrotrophic effector triggering a hypersensitive response in susceptible rubber clones, in agreement with the necrotrophic-effector-triggered susceptibility model.Sébastien RibeiroPhilippe LabelDominique GarciaPascal MontoroValérie Pujade-RenaudPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 7, p e0254541 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sébastien Ribeiro Philippe Label Dominique Garcia Pascal Montoro Valérie Pujade-Renaud Transcriptome profiling in susceptible and tolerant rubber tree clones in response to cassiicolin Cas1, a necrotrophic effector from Corynespora cassiicola.
description	Corynespora cassiicola, a fungal plant pathogen with a large host range, causes important damages in rubber tree (Hevea brasiliensis), in Asia and Africa. A small secreted protein named cassiicolin was previously identified as a necrotrophic effector required for the virulence of C. cassiicola in specific rubber tree clones. The objective of this study was to decipher the cassiicolin-mediated molecular mechanisms involved in this compatible interaction. We comparatively analyzed the RNA-Seq transcriptomic profiles of leaves treated or not with the purified cassiicolin Cas1, in two rubber clones: PB260 (susceptible) and RRIM600 (tolerant). The reads were mapped against a synthetic transcriptome composed of all available transcriptomic references from the two clones. Genes differentially expressed in response to cassiicolin Cas1 were identified, in each clone, at two different time-points. After de novo annotation of the synthetic transcriptome, we analyzed GO enrichment of the differentially expressed genes in order to elucidate the main functional pathways impacted by cassiicolin. Cassiicolin induced qualitatively similar transcriptional modifications in both the susceptible and the tolerant clones, with a strong negative impact on photosynthesis, and the activation of defense responses via redox signaling, production of pathogenesis-related protein, or activation of the secondary metabolism. In the tolerant clone, transcriptional reprogramming occurred earlier but remained moderate. By contrast, the susceptible clone displayed a late but huge transcriptional burst, characterized by massive induction of phosphorylation events and all the features of a hypersensitive response. These results confirm that cassiicolin Cas1 is a necrotrophic effector triggering a hypersensitive response in susceptible rubber clones, in agreement with the necrotrophic-effector-triggered susceptibility model.
format	article
author	Sébastien Ribeiro Philippe Label Dominique Garcia Pascal Montoro Valérie Pujade-Renaud
author_facet	Sébastien Ribeiro Philippe Label Dominique Garcia Pascal Montoro Valérie Pujade-Renaud
author_sort	Sébastien Ribeiro
title	Transcriptome profiling in susceptible and tolerant rubber tree clones in response to cassiicolin Cas1, a necrotrophic effector from Corynespora cassiicola.
title_short	Transcriptome profiling in susceptible and tolerant rubber tree clones in response to cassiicolin Cas1, a necrotrophic effector from Corynespora cassiicola.
title_full	Transcriptome profiling in susceptible and tolerant rubber tree clones in response to cassiicolin Cas1, a necrotrophic effector from Corynespora cassiicola.
title_fullStr	Transcriptome profiling in susceptible and tolerant rubber tree clones in response to cassiicolin Cas1, a necrotrophic effector from Corynespora cassiicola.
title_full_unstemmed	Transcriptome profiling in susceptible and tolerant rubber tree clones in response to cassiicolin Cas1, a necrotrophic effector from Corynespora cassiicola.
title_sort	transcriptome profiling in susceptible and tolerant rubber tree clones in response to cassiicolin cas1, a necrotrophic effector from corynespora cassiicola.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/db4e5e980f584ed983d48f69ec32ccb2
work_keys_str_mv	AT sebastienribeiro transcriptomeprofilinginsusceptibleandtolerantrubbertreeclonesinresponsetocassiicolincas1anecrotrophiceffectorfromcorynesporacassiicola AT philippelabel transcriptomeprofilinginsusceptibleandtolerantrubbertreeclonesinresponsetocassiicolincas1anecrotrophiceffectorfromcorynesporacassiicola AT dominiquegarcia transcriptomeprofilinginsusceptibleandtolerantrubbertreeclonesinresponsetocassiicolincas1anecrotrophiceffectorfromcorynesporacassiicola AT pascalmontoro transcriptomeprofilinginsusceptibleandtolerantrubbertreeclonesinresponsetocassiicolincas1anecrotrophiceffectorfromcorynesporacassiicola AT valeriepujaderenaud transcriptomeprofilinginsusceptibleandtolerantrubbertreeclonesinresponsetocassiicolincas1anecrotrophiceffectorfromcorynesporacassiicola
_version_	1718375154429460480

Transcriptome profiling in susceptible and tolerant rubber tree clones in response to cassiicolin Cas1, a necrotrophic effector from Corynespora cassiicola.

Ejemplares similares