In situ functional dissection of RNA cis-regulatory elements by multiplex CRISPR-Cas9 genome engineering

RNA regulatory elements (RREs) are important post-transcriptional control features but studying them requires disrupting their activity without disturbing cellular homeostasis. Here the authors present GenERA, a CRISPR-Cas9 screening platform of in situ analysis of native RREs.

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Autores principales:	Qianxin Wu, Quentin R. V. Ferry, Toni A. Baeumler, Yale S. Michaels, Dimitrios M. Vitsios, Omer Habib, Roland Arnold, Xiaowei Jiang, Stefano Maio, Bruno R. Steinkraus, Marta Tapia, Paolo Piazza, Ni Xu, Georg A. Holländer, Thomas A. Milne, Jin-Soo Kim, Anton J. Enright, Andrew R. Bassett, Tudor A. Fulga
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/db4eb21f42e2466d9fc7c9b4e4aa2f37
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spelling	oai:doaj.org-article:db4eb21f42e2466d9fc7c9b4e4aa2f372021-12-02T14:42:04ZIn situ functional dissection of RNA cis-regulatory elements by multiplex CRISPR-Cas9 genome engineering10.1038/s41467-017-00686-22041-1723https://doaj.org/article/db4eb21f42e2466d9fc7c9b4e4aa2f372017-12-01T00:00:00Zhttps://doi.org/10.1038/s41467-017-00686-2https://doaj.org/toc/2041-1723RNA regulatory elements (RREs) are important post-transcriptional control features but studying them requires disrupting their activity without disturbing cellular homeostasis. Here the authors present GenERA, a CRISPR-Cas9 screening platform of in situ analysis of native RREs.Qianxin WuQuentin R. V. FerryToni A. BaeumlerYale S. MichaelsDimitrios M. VitsiosOmer HabibRoland ArnoldXiaowei JiangStefano MaioBruno R. SteinkrausMarta TapiaPaolo PiazzaNi XuGeorg A. HolländerThomas A. MilneJin-Soo KimAnton J. EnrightAndrew R. BassettTudor A. FulgaNature PortfolioarticleScienceQENNature Communications, Vol 8, Iss 1, Pp 1-17 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Qianxin Wu Quentin R. V. Ferry Toni A. Baeumler Yale S. Michaels Dimitrios M. Vitsios Omer Habib Roland Arnold Xiaowei Jiang Stefano Maio Bruno R. Steinkraus Marta Tapia Paolo Piazza Ni Xu Georg A. Holländer Thomas A. Milne Jin-Soo Kim Anton J. Enright Andrew R. Bassett Tudor A. Fulga In situ functional dissection of RNA cis-regulatory elements by multiplex CRISPR-Cas9 genome engineering
description	RNA regulatory elements (RREs) are important post-transcriptional control features but studying them requires disrupting their activity without disturbing cellular homeostasis. Here the authors present GenERA, a CRISPR-Cas9 screening platform of in situ analysis of native RREs.
format	article
author	Qianxin Wu Quentin R. V. Ferry Toni A. Baeumler Yale S. Michaels Dimitrios M. Vitsios Omer Habib Roland Arnold Xiaowei Jiang Stefano Maio Bruno R. Steinkraus Marta Tapia Paolo Piazza Ni Xu Georg A. Holländer Thomas A. Milne Jin-Soo Kim Anton J. Enright Andrew R. Bassett Tudor A. Fulga
author_facet	Qianxin Wu Quentin R. V. Ferry Toni A. Baeumler Yale S. Michaels Dimitrios M. Vitsios Omer Habib Roland Arnold Xiaowei Jiang Stefano Maio Bruno R. Steinkraus Marta Tapia Paolo Piazza Ni Xu Georg A. Holländer Thomas A. Milne Jin-Soo Kim Anton J. Enright Andrew R. Bassett Tudor A. Fulga
author_sort	Qianxin Wu
title	In situ functional dissection of RNA cis-regulatory elements by multiplex CRISPR-Cas9 genome engineering
title_short	In situ functional dissection of RNA cis-regulatory elements by multiplex CRISPR-Cas9 genome engineering
title_full	In situ functional dissection of RNA cis-regulatory elements by multiplex CRISPR-Cas9 genome engineering
title_fullStr	In situ functional dissection of RNA cis-regulatory elements by multiplex CRISPR-Cas9 genome engineering
title_full_unstemmed	In situ functional dissection of RNA cis-regulatory elements by multiplex CRISPR-Cas9 genome engineering
title_sort	in situ functional dissection of rna cis-regulatory elements by multiplex crispr-cas9 genome engineering
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/db4eb21f42e2466d9fc7c9b4e4aa2f37
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In situ functional dissection of RNA cis-regulatory elements by multiplex CRISPR-Cas9 genome engineering

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