Detection and removal of barcode swapping in single-cell RNA-seq data

DNA barcode swapping results in mislabelling of sequencing reads between multiplexed samples. Here, the authors investigate the severity and consequences of barcode swapping for single-cell RNA-seq data, and develop a computational method to exclude swapped reads.

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Autores principales:	Jonathan A. Griffiths, Arianne C. Richard, Karsten Bach, Aaron T. L. Lun, John C. Marioni
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/db4ec97f2eca41a5b07146e356af43b5
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spelling	oai:doaj.org-article:db4ec97f2eca41a5b07146e356af43b52021-12-02T15:34:42ZDetection and removal of barcode swapping in single-cell RNA-seq data10.1038/s41467-018-05083-x2041-1723https://doaj.org/article/db4ec97f2eca41a5b07146e356af43b52018-07-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-05083-xhttps://doaj.org/toc/2041-1723DNA barcode swapping results in mislabelling of sequencing reads between multiplexed samples. Here, the authors investigate the severity and consequences of barcode swapping for single-cell RNA-seq data, and develop a computational method to exclude swapped reads.Jonathan A. GriffithsArianne C. RichardKarsten BachAaron T. L. LunJohn C. MarioniNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-6 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Jonathan A. Griffiths Arianne C. Richard Karsten Bach Aaron T. L. Lun John C. Marioni Detection and removal of barcode swapping in single-cell RNA-seq data
description	DNA barcode swapping results in mislabelling of sequencing reads between multiplexed samples. Here, the authors investigate the severity and consequences of barcode swapping for single-cell RNA-seq data, and develop a computational method to exclude swapped reads.
format	article
author	Jonathan A. Griffiths Arianne C. Richard Karsten Bach Aaron T. L. Lun John C. Marioni
author_facet	Jonathan A. Griffiths Arianne C. Richard Karsten Bach Aaron T. L. Lun John C. Marioni
author_sort	Jonathan A. Griffiths
title	Detection and removal of barcode swapping in single-cell RNA-seq data
title_short	Detection and removal of barcode swapping in single-cell RNA-seq data
title_full	Detection and removal of barcode swapping in single-cell RNA-seq data
title_fullStr	Detection and removal of barcode swapping in single-cell RNA-seq data
title_full_unstemmed	Detection and removal of barcode swapping in single-cell RNA-seq data
title_sort	detection and removal of barcode swapping in single-cell rna-seq data
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/db4ec97f2eca41a5b07146e356af43b5
work_keys_str_mv	AT jonathanagriffiths detectionandremovalofbarcodeswappinginsinglecellrnaseqdata AT ariannecrichard detectionandremovalofbarcodeswappinginsinglecellrnaseqdata AT karstenbach detectionandremovalofbarcodeswappinginsinglecellrnaseqdata AT aarontllun detectionandremovalofbarcodeswappinginsinglecellrnaseqdata AT johncmarioni detectionandremovalofbarcodeswappinginsinglecellrnaseqdata
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Detection and removal of barcode swapping in single-cell RNA-seq data

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