A database on differentially expressed microRNAs during rodent bladder healing

Abstract Urinary bladder wound healing relies on multiple biological events that are finely tuned in a spatial–temporal manner. MicroRNAs are small non-coding RNA molecules with regulatory functions. We hypothesized that microRNAs are important molecules in the coordination of normal urinary bladder...

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Autores principales:	Clara Ibel Chamorro, Jesper Eisfeldt, Oliver Willacy, Nikolai Juul, Magdalena Fossum
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/db5a71cc335841ce9a7015710b38efc6
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spelling	oai:doaj.org-article:db5a71cc335841ce9a7015710b38efc62021-11-14T12:21:07ZA database on differentially expressed microRNAs during rodent bladder healing10.1038/s41598-021-01413-02045-2322https://doaj.org/article/db5a71cc335841ce9a7015710b38efc62021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-01413-0https://doaj.org/toc/2045-2322Abstract Urinary bladder wound healing relies on multiple biological events that are finely tuned in a spatial–temporal manner. MicroRNAs are small non-coding RNA molecules with regulatory functions. We hypothesized that microRNAs are important molecules in the coordination of normal urinary bladder wound healing. We aimed at identifying microRNAs expressed during bladder wound healing using Affymetrix global array for microRNA profiling of the rodent urinary bladder during healing of a surgically created wound. Results were validated in the rat bladders by real-time PCR (RT-PCR) using three of the differentially expressed (DE) microRNAs. The model was thereafter validated in human cells, by measuring the expression of eight of the DE microRNAs upon in vitro wound-healing assays in primary urothelial cells. Our results indicated that 508 (40%) of all rodent microRNAs were expressed in the urinary bladder during wound healing. Thirteen of these microRNAs (1%) were DE (false discovery rate (FDR) < 0.05, P < 0.05, \|logfold\|> 0.25) in wounded compared to non-wounded bladders. Bioinformatic analyses helped us to identify target molecules for the DE microRNAs, and biological pathways involved in tissue repair. All data are made available in an open-access database for other researchers to explore.Clara Ibel ChamorroJesper EisfeldtOliver WillacyNikolai JuulMagdalena FossumNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Clara Ibel Chamorro Jesper Eisfeldt Oliver Willacy Nikolai Juul Magdalena Fossum A database on differentially expressed microRNAs during rodent bladder healing
description	Abstract Urinary bladder wound healing relies on multiple biological events that are finely tuned in a spatial–temporal manner. MicroRNAs are small non-coding RNA molecules with regulatory functions. We hypothesized that microRNAs are important molecules in the coordination of normal urinary bladder wound healing. We aimed at identifying microRNAs expressed during bladder wound healing using Affymetrix global array for microRNA profiling of the rodent urinary bladder during healing of a surgically created wound. Results were validated in the rat bladders by real-time PCR (RT-PCR) using three of the differentially expressed (DE) microRNAs. The model was thereafter validated in human cells, by measuring the expression of eight of the DE microRNAs upon in vitro wound-healing assays in primary urothelial cells. Our results indicated that 508 (40%) of all rodent microRNAs were expressed in the urinary bladder during wound healing. Thirteen of these microRNAs (1%) were DE (false discovery rate (FDR) < 0.05, P < 0.05, \|logfold\|> 0.25) in wounded compared to non-wounded bladders. Bioinformatic analyses helped us to identify target molecules for the DE microRNAs, and biological pathways involved in tissue repair. All data are made available in an open-access database for other researchers to explore.
format	article
author	Clara Ibel Chamorro Jesper Eisfeldt Oliver Willacy Nikolai Juul Magdalena Fossum
author_facet	Clara Ibel Chamorro Jesper Eisfeldt Oliver Willacy Nikolai Juul Magdalena Fossum
author_sort	Clara Ibel Chamorro
title	A database on differentially expressed microRNAs during rodent bladder healing
title_short	A database on differentially expressed microRNAs during rodent bladder healing
title_full	A database on differentially expressed microRNAs during rodent bladder healing
title_fullStr	A database on differentially expressed microRNAs during rodent bladder healing
title_full_unstemmed	A database on differentially expressed microRNAs during rodent bladder healing
title_sort	database on differentially expressed micrornas during rodent bladder healing
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/db5a71cc335841ce9a7015710b38efc6
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A database on differentially expressed microRNAs during rodent bladder healing

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