Screening of viral-vectored P. falciparum pre-erythrocytic candidate vaccine antigens using chimeric rodent parasites.

To screen for additional vaccine candidate antigens of Plasmodium pre-erythrocytic stages, fourteen P. falciparum proteins were selected based on expression in sporozoites or their role in establishment of hepatocyte infection. For preclinical evaluation of immunogenicity of these proteins in mice,...

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Autores principales: Surendra Kumar Kolli, Ahmed M Salman, Jai Ramesar, Severine Chevalley-Maurel, Hans Kroeze, Fiona G A Geurten, Shinya Miyazaki, Ekta Mukhopadhyay, Catherin Marin-Mogollon, Blandine Franke-Fayard, Adrian V S Hill, Chris J Janse
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Publicado: Public Library of Science (PLoS) 2021
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spelling oai:doaj.org-article:db5e2deb62c14c5b94c90aa399369d2e2021-12-02T20:15:28ZScreening of viral-vectored P. falciparum pre-erythrocytic candidate vaccine antigens using chimeric rodent parasites.1932-620310.1371/journal.pone.0254498https://doaj.org/article/db5e2deb62c14c5b94c90aa399369d2e2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0254498https://doaj.org/toc/1932-6203To screen for additional vaccine candidate antigens of Plasmodium pre-erythrocytic stages, fourteen P. falciparum proteins were selected based on expression in sporozoites or their role in establishment of hepatocyte infection. For preclinical evaluation of immunogenicity of these proteins in mice, chimeric P. berghei sporozoites were created that express the P. falciparum proteins in sporozoites as an additional copy gene under control of the uis4 gene promoter. All fourteen chimeric parasites produced sporozoites but sporozoites of eight lines failed to establish a liver infection, indicating a negative impact of these P. falciparum proteins on sporozoite infectivity. Immunogenicity of the other six proteins (SPELD, ETRAMP10.3, SIAP2, SPATR, HT, RPL3) was analyzed by immunization of inbred BALB/c and outbred CD-1 mice with viral-vectored (ChAd63 or ChAdOx1, MVA) vaccines, followed by challenge with chimeric sporozoites. Protective immunogenicity was determined by analyzing parasite liver load and prepatent period of blood stage infection after challenge. Of the six proteins only SPELD immunized mice showed partial protection. We discuss both the low protective immunogenicity of these proteins in the chimeric rodent malaria challenge model and the negative effect on P. berghei sporozoite infectivity of several P. falciparum proteins expressed in the chimeric sporozoites.Surendra Kumar KolliAhmed M SalmanJai RamesarSeverine Chevalley-MaurelHans KroezeFiona G A GeurtenShinya MiyazakiEkta MukhopadhyayCatherin Marin-MogollonBlandine Franke-FayardAdrian V S HillChris J JansePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 7, p e0254498 (2021)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Surendra Kumar Kolli
Ahmed M Salman
Jai Ramesar
Severine Chevalley-Maurel
Hans Kroeze
Fiona G A Geurten
Shinya Miyazaki
Ekta Mukhopadhyay
Catherin Marin-Mogollon
Blandine Franke-Fayard
Adrian V S Hill
Chris J Janse
Screening of viral-vectored P. falciparum pre-erythrocytic candidate vaccine antigens using chimeric rodent parasites.
description To screen for additional vaccine candidate antigens of Plasmodium pre-erythrocytic stages, fourteen P. falciparum proteins were selected based on expression in sporozoites or their role in establishment of hepatocyte infection. For preclinical evaluation of immunogenicity of these proteins in mice, chimeric P. berghei sporozoites were created that express the P. falciparum proteins in sporozoites as an additional copy gene under control of the uis4 gene promoter. All fourteen chimeric parasites produced sporozoites but sporozoites of eight lines failed to establish a liver infection, indicating a negative impact of these P. falciparum proteins on sporozoite infectivity. Immunogenicity of the other six proteins (SPELD, ETRAMP10.3, SIAP2, SPATR, HT, RPL3) was analyzed by immunization of inbred BALB/c and outbred CD-1 mice with viral-vectored (ChAd63 or ChAdOx1, MVA) vaccines, followed by challenge with chimeric sporozoites. Protective immunogenicity was determined by analyzing parasite liver load and prepatent period of blood stage infection after challenge. Of the six proteins only SPELD immunized mice showed partial protection. We discuss both the low protective immunogenicity of these proteins in the chimeric rodent malaria challenge model and the negative effect on P. berghei sporozoite infectivity of several P. falciparum proteins expressed in the chimeric sporozoites.
format article
author Surendra Kumar Kolli
Ahmed M Salman
Jai Ramesar
Severine Chevalley-Maurel
Hans Kroeze
Fiona G A Geurten
Shinya Miyazaki
Ekta Mukhopadhyay
Catherin Marin-Mogollon
Blandine Franke-Fayard
Adrian V S Hill
Chris J Janse
author_facet Surendra Kumar Kolli
Ahmed M Salman
Jai Ramesar
Severine Chevalley-Maurel
Hans Kroeze
Fiona G A Geurten
Shinya Miyazaki
Ekta Mukhopadhyay
Catherin Marin-Mogollon
Blandine Franke-Fayard
Adrian V S Hill
Chris J Janse
author_sort Surendra Kumar Kolli
title Screening of viral-vectored P. falciparum pre-erythrocytic candidate vaccine antigens using chimeric rodent parasites.
title_short Screening of viral-vectored P. falciparum pre-erythrocytic candidate vaccine antigens using chimeric rodent parasites.
title_full Screening of viral-vectored P. falciparum pre-erythrocytic candidate vaccine antigens using chimeric rodent parasites.
title_fullStr Screening of viral-vectored P. falciparum pre-erythrocytic candidate vaccine antigens using chimeric rodent parasites.
title_full_unstemmed Screening of viral-vectored P. falciparum pre-erythrocytic candidate vaccine antigens using chimeric rodent parasites.
title_sort screening of viral-vectored p. falciparum pre-erythrocytic candidate vaccine antigens using chimeric rodent parasites.
publisher Public Library of Science (PLoS)
publishDate 2021
url https://doaj.org/article/db5e2deb62c14c5b94c90aa399369d2e
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