Screening of viral-vectored P. falciparum pre-erythrocytic candidate vaccine antigens using chimeric rodent parasites.

To screen for additional vaccine candidate antigens of Plasmodium pre-erythrocytic stages, fourteen P. falciparum proteins were selected based on expression in sporozoites or their role in establishment of hepatocyte infection. For preclinical evaluation of immunogenicity of these proteins in mice,...

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Auteurs principaux:	Surendra Kumar Kolli, Ahmed M Salman, Jai Ramesar, Severine Chevalley-Maurel, Hans Kroeze, Fiona G A Geurten, Shinya Miyazaki, Ekta Mukhopadhyay, Catherin Marin-Mogollon, Blandine Franke-Fayard, Adrian V S Hill, Chris J Janse
Format:	article
Langue:	EN
Publié:	Public Library of Science (PLoS) 2021
Sujets:	Medicine R Science Q
Accès en ligne:	https://doaj.org/article/db5e2deb62c14c5b94c90aa399369d2e
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spelling	oai:doaj.org-article:db5e2deb62c14c5b94c90aa399369d2e2021-12-02T20:15:28ZScreening of viral-vectored P. falciparum pre-erythrocytic candidate vaccine antigens using chimeric rodent parasites.1932-620310.1371/journal.pone.0254498https://doaj.org/article/db5e2deb62c14c5b94c90aa399369d2e2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0254498https://doaj.org/toc/1932-6203To screen for additional vaccine candidate antigens of Plasmodium pre-erythrocytic stages, fourteen P. falciparum proteins were selected based on expression in sporozoites or their role in establishment of hepatocyte infection. For preclinical evaluation of immunogenicity of these proteins in mice, chimeric P. berghei sporozoites were created that express the P. falciparum proteins in sporozoites as an additional copy gene under control of the uis4 gene promoter. All fourteen chimeric parasites produced sporozoites but sporozoites of eight lines failed to establish a liver infection, indicating a negative impact of these P. falciparum proteins on sporozoite infectivity. Immunogenicity of the other six proteins (SPELD, ETRAMP10.3, SIAP2, SPATR, HT, RPL3) was analyzed by immunization of inbred BALB/c and outbred CD-1 mice with viral-vectored (ChAd63 or ChAdOx1, MVA) vaccines, followed by challenge with chimeric sporozoites. Protective immunogenicity was determined by analyzing parasite liver load and prepatent period of blood stage infection after challenge. Of the six proteins only SPELD immunized mice showed partial protection. We discuss both the low protective immunogenicity of these proteins in the chimeric rodent malaria challenge model and the negative effect on P. berghei sporozoite infectivity of several P. falciparum proteins expressed in the chimeric sporozoites.Surendra Kumar KolliAhmed M SalmanJai RamesarSeverine Chevalley-MaurelHans KroezeFiona G A GeurtenShinya MiyazakiEkta MukhopadhyayCatherin Marin-MogollonBlandine Franke-FayardAdrian V S HillChris J JansePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 7, p e0254498 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Surendra Kumar Kolli Ahmed M Salman Jai Ramesar Severine Chevalley-Maurel Hans Kroeze Fiona G A Geurten Shinya Miyazaki Ekta Mukhopadhyay Catherin Marin-Mogollon Blandine Franke-Fayard Adrian V S Hill Chris J Janse Screening of viral-vectored P. falciparum pre-erythrocytic candidate vaccine antigens using chimeric rodent parasites.
description	To screen for additional vaccine candidate antigens of Plasmodium pre-erythrocytic stages, fourteen P. falciparum proteins were selected based on expression in sporozoites or their role in establishment of hepatocyte infection. For preclinical evaluation of immunogenicity of these proteins in mice, chimeric P. berghei sporozoites were created that express the P. falciparum proteins in sporozoites as an additional copy gene under control of the uis4 gene promoter. All fourteen chimeric parasites produced sporozoites but sporozoites of eight lines failed to establish a liver infection, indicating a negative impact of these P. falciparum proteins on sporozoite infectivity. Immunogenicity of the other six proteins (SPELD, ETRAMP10.3, SIAP2, SPATR, HT, RPL3) was analyzed by immunization of inbred BALB/c and outbred CD-1 mice with viral-vectored (ChAd63 or ChAdOx1, MVA) vaccines, followed by challenge with chimeric sporozoites. Protective immunogenicity was determined by analyzing parasite liver load and prepatent period of blood stage infection after challenge. Of the six proteins only SPELD immunized mice showed partial protection. We discuss both the low protective immunogenicity of these proteins in the chimeric rodent malaria challenge model and the negative effect on P. berghei sporozoite infectivity of several P. falciparum proteins expressed in the chimeric sporozoites.
format	article
author	Surendra Kumar Kolli Ahmed M Salman Jai Ramesar Severine Chevalley-Maurel Hans Kroeze Fiona G A Geurten Shinya Miyazaki Ekta Mukhopadhyay Catherin Marin-Mogollon Blandine Franke-Fayard Adrian V S Hill Chris J Janse
author_facet	Surendra Kumar Kolli Ahmed M Salman Jai Ramesar Severine Chevalley-Maurel Hans Kroeze Fiona G A Geurten Shinya Miyazaki Ekta Mukhopadhyay Catherin Marin-Mogollon Blandine Franke-Fayard Adrian V S Hill Chris J Janse
author_sort	Surendra Kumar Kolli
title	Screening of viral-vectored P. falciparum pre-erythrocytic candidate vaccine antigens using chimeric rodent parasites.
title_short	Screening of viral-vectored P. falciparum pre-erythrocytic candidate vaccine antigens using chimeric rodent parasites.
title_full	Screening of viral-vectored P. falciparum pre-erythrocytic candidate vaccine antigens using chimeric rodent parasites.
title_fullStr	Screening of viral-vectored P. falciparum pre-erythrocytic candidate vaccine antigens using chimeric rodent parasites.
title_full_unstemmed	Screening of viral-vectored P. falciparum pre-erythrocytic candidate vaccine antigens using chimeric rodent parasites.
title_sort	screening of viral-vectored p. falciparum pre-erythrocytic candidate vaccine antigens using chimeric rodent parasites.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/db5e2deb62c14c5b94c90aa399369d2e
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Screening of viral-vectored P. falciparum pre-erythrocytic candidate vaccine antigens using chimeric rodent parasites.

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