Determination of 35 Free Amino Acids in Tea Using Ultra-Performance Liquid Chromatography Coupled With Quadrupole Time-of-Flight Mass Spectrometry

The purpose of this research was to develop a simple, sensitive, and accurate method for simultaneous determination of 35 free amino acids using ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS). Tea samples were extracted with boiling w...

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Autores principales:	Jian Li, Junmei Ma, Qiang Li, Sufang Fan, Lixin Fan, Hongyu Ma, Yan Zhang, Lei Zheng
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	ultra-performance liquid chromatography quadrupole-time of flight mass spectrometry tea free amino acids detection Nutrition. Foods and food supply TX341-641
Acceso en línea:	https://doaj.org/article/db5ee53bbdcc4530b6c9d959d1b6b50b
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spelling	oai:doaj.org-article:db5ee53bbdcc4530b6c9d959d1b6b50b2021-12-01T23:20:56ZDetermination of 35 Free Amino Acids in Tea Using Ultra-Performance Liquid Chromatography Coupled With Quadrupole Time-of-Flight Mass Spectrometry2296-861X10.3389/fnut.2021.767801https://doaj.org/article/db5ee53bbdcc4530b6c9d959d1b6b50b2021-12-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fnut.2021.767801/fullhttps://doaj.org/toc/2296-861XThe purpose of this research was to develop a simple, sensitive, and accurate method for simultaneous determination of 35 free amino acids using ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS). Tea samples were extracted with boiling water bath, and then separated by XBridge BEH Amide column by gradient elution. The exact mass and MS/MS spectra of the target compound was detected under the TOF–MS and Information dependent acquisition (IDA)–MS/MS mode. The results demonstrated good linearity (R2 > 0.9980) in the range of 0.5–1,000 ng/mL. The limits of detection (LODs) were 0.13–25.00 mg/kg and the limits of quantitation (LOQs) were 0.25–50.00 mg/kg. The recovery rate ranged from 70.1 to 105.1% with relative standard deviations (RSDs) <11% (n = 6). This research provides a targeted strategy for developing an analysis method for amino acids in tea.Jian LiJian LiJunmei MaJunmei MaQiang LiSufang FanLixin FanHongyu MaYan ZhangYan ZhangLei ZhengFrontiers Media S.A.articleultra-performance liquid chromatographyquadrupole-time of flight mass spectrometryteafree amino acidsdetectionNutrition. Foods and food supplyTX341-641ENFrontiers in Nutrition, Vol 8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	ultra-performance liquid chromatography quadrupole-time of flight mass spectrometry tea free amino acids detection Nutrition. Foods and food supply TX341-641
spellingShingle	ultra-performance liquid chromatography quadrupole-time of flight mass spectrometry tea free amino acids detection Nutrition. Foods and food supply TX341-641 Jian Li Jian Li Junmei Ma Junmei Ma Qiang Li Sufang Fan Lixin Fan Hongyu Ma Yan Zhang Yan Zhang Lei Zheng Determination of 35 Free Amino Acids in Tea Using Ultra-Performance Liquid Chromatography Coupled With Quadrupole Time-of-Flight Mass Spectrometry
description	The purpose of this research was to develop a simple, sensitive, and accurate method for simultaneous determination of 35 free amino acids using ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS). Tea samples were extracted with boiling water bath, and then separated by XBridge BEH Amide column by gradient elution. The exact mass and MS/MS spectra of the target compound was detected under the TOF–MS and Information dependent acquisition (IDA)–MS/MS mode. The results demonstrated good linearity (R2 > 0.9980) in the range of 0.5–1,000 ng/mL. The limits of detection (LODs) were 0.13–25.00 mg/kg and the limits of quantitation (LOQs) were 0.25–50.00 mg/kg. The recovery rate ranged from 70.1 to 105.1% with relative standard deviations (RSDs) <11% (n = 6). This research provides a targeted strategy for developing an analysis method for amino acids in tea.
format	article
author	Jian Li Jian Li Junmei Ma Junmei Ma Qiang Li Sufang Fan Lixin Fan Hongyu Ma Yan Zhang Yan Zhang Lei Zheng
author_facet	Jian Li Jian Li Junmei Ma Junmei Ma Qiang Li Sufang Fan Lixin Fan Hongyu Ma Yan Zhang Yan Zhang Lei Zheng
author_sort	Jian Li
title	Determination of 35 Free Amino Acids in Tea Using Ultra-Performance Liquid Chromatography Coupled With Quadrupole Time-of-Flight Mass Spectrometry
title_short	Determination of 35 Free Amino Acids in Tea Using Ultra-Performance Liquid Chromatography Coupled With Quadrupole Time-of-Flight Mass Spectrometry
title_full	Determination of 35 Free Amino Acids in Tea Using Ultra-Performance Liquid Chromatography Coupled With Quadrupole Time-of-Flight Mass Spectrometry
title_fullStr	Determination of 35 Free Amino Acids in Tea Using Ultra-Performance Liquid Chromatography Coupled With Quadrupole Time-of-Flight Mass Spectrometry
title_full_unstemmed	Determination of 35 Free Amino Acids in Tea Using Ultra-Performance Liquid Chromatography Coupled With Quadrupole Time-of-Flight Mass Spectrometry
title_sort	determination of 35 free amino acids in tea using ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/db5ee53bbdcc4530b6c9d959d1b6b50b
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Determination of 35 Free Amino Acids in Tea Using Ultra-Performance Liquid Chromatography Coupled With Quadrupole Time-of-Flight Mass Spectrometry

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