Diet-induced reconstruction of mucosal microbiota associated with alterations of epithelium lectin expression and regulation in the maintenance of rumen homeostasis

Abstract It is unknown whether lectins of the rumen epithelium contribute to the recognition of mucosal microbes and activation of tolerogenic cytokines in ruminant animals. We applied an integrated method of RNA-seq and 16S rRNA gene sequencing to investigate alterations of epithelial lectin expres...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Hong Shen, Zhongyan Lu, Zhihui Xu, Zanming Shen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/db6cc5da3a3b4aa38d42e0b2a2c6f8cf
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:db6cc5da3a3b4aa38d42e0b2a2c6f8cf
record_format	dspace
spelling	oai:doaj.org-article:db6cc5da3a3b4aa38d42e0b2a2c6f8cf2021-12-02T12:30:13ZDiet-induced reconstruction of mucosal microbiota associated with alterations of epithelium lectin expression and regulation in the maintenance of rumen homeostasis10.1038/s41598-017-03478-22045-2322https://doaj.org/article/db6cc5da3a3b4aa38d42e0b2a2c6f8cf2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03478-2https://doaj.org/toc/2045-2322Abstract It is unknown whether lectins of the rumen epithelium contribute to the recognition of mucosal microbes and activation of tolerogenic cytokines in ruminant animals. We applied an integrated method of RNA-seq and 16S rRNA gene sequencing to investigate alterations of epithelial lectin expression and regulation with a diet-induced reconstruction of the mucosal microbiota in the goat rumen. Our results showed that the diversity and richness of the rumen mucosal microbiota were promoted by the dietary concentrate. Meantime, in the rumen epithelium, five lectin genes, namely, sialic acid-binding Ig-like lectin 14 (LOC102180073), C-type lectin domain family 4, member E (CLEC4E), C-type lectin domain family 7, member A (CLEC7A), C-type lectin domain family 16, member A (CLEC16A), and lectin, mannose-binding 2 (LMAN2), were indicated to promote the expression of 8 tolerogenic cytokines, transforming growth factor beta 1 (TGFB1) and 4 enzyme genes involved in retinoic acid biosynthesis via 6 signaling pathways. Analysis of the combined data showed that 9 microbial genera (Clostridium_IV, Desulfobulbus, Eubacterium, Ochrobactrum, Propionibacterium, Pseudomonas, Slackia, Staphylococcus and Subdivision5_genera_IS) were highly related to the expression of functional lectins. These findings provide new insights into the interactions between the rumen epithelium and mucosal microbiota in the maintenance of rumen homeostasis.Hong ShenZhongyan LuZhihui XuZanming ShenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hong Shen Zhongyan Lu Zhihui Xu Zanming Shen Diet-induced reconstruction of mucosal microbiota associated with alterations of epithelium lectin expression and regulation in the maintenance of rumen homeostasis
description	Abstract It is unknown whether lectins of the rumen epithelium contribute to the recognition of mucosal microbes and activation of tolerogenic cytokines in ruminant animals. We applied an integrated method of RNA-seq and 16S rRNA gene sequencing to investigate alterations of epithelial lectin expression and regulation with a diet-induced reconstruction of the mucosal microbiota in the goat rumen. Our results showed that the diversity and richness of the rumen mucosal microbiota were promoted by the dietary concentrate. Meantime, in the rumen epithelium, five lectin genes, namely, sialic acid-binding Ig-like lectin 14 (LOC102180073), C-type lectin domain family 4, member E (CLEC4E), C-type lectin domain family 7, member A (CLEC7A), C-type lectin domain family 16, member A (CLEC16A), and lectin, mannose-binding 2 (LMAN2), were indicated to promote the expression of 8 tolerogenic cytokines, transforming growth factor beta 1 (TGFB1) and 4 enzyme genes involved in retinoic acid biosynthesis via 6 signaling pathways. Analysis of the combined data showed that 9 microbial genera (Clostridium_IV, Desulfobulbus, Eubacterium, Ochrobactrum, Propionibacterium, Pseudomonas, Slackia, Staphylococcus and Subdivision5_genera_IS) were highly related to the expression of functional lectins. These findings provide new insights into the interactions between the rumen epithelium and mucosal microbiota in the maintenance of rumen homeostasis.
format	article
author	Hong Shen Zhongyan Lu Zhihui Xu Zanming Shen
author_facet	Hong Shen Zhongyan Lu Zhihui Xu Zanming Shen
author_sort	Hong Shen
title	Diet-induced reconstruction of mucosal microbiota associated with alterations of epithelium lectin expression and regulation in the maintenance of rumen homeostasis
title_short	Diet-induced reconstruction of mucosal microbiota associated with alterations of epithelium lectin expression and regulation in the maintenance of rumen homeostasis
title_full	Diet-induced reconstruction of mucosal microbiota associated with alterations of epithelium lectin expression and regulation in the maintenance of rumen homeostasis
title_fullStr	Diet-induced reconstruction of mucosal microbiota associated with alterations of epithelium lectin expression and regulation in the maintenance of rumen homeostasis
title_full_unstemmed	Diet-induced reconstruction of mucosal microbiota associated with alterations of epithelium lectin expression and regulation in the maintenance of rumen homeostasis
title_sort	diet-induced reconstruction of mucosal microbiota associated with alterations of epithelium lectin expression and regulation in the maintenance of rumen homeostasis
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/db6cc5da3a3b4aa38d42e0b2a2c6f8cf
work_keys_str_mv	AT hongshen dietinducedreconstructionofmucosalmicrobiotaassociatedwithalterationsofepitheliumlectinexpressionandregulationinthemaintenanceofrumenhomeostasis AT zhongyanlu dietinducedreconstructionofmucosalmicrobiotaassociatedwithalterationsofepitheliumlectinexpressionandregulationinthemaintenanceofrumenhomeostasis AT zhihuixu dietinducedreconstructionofmucosalmicrobiotaassociatedwithalterationsofepitheliumlectinexpressionandregulationinthemaintenanceofrumenhomeostasis AT zanmingshen dietinducedreconstructionofmucosalmicrobiotaassociatedwithalterationsofepitheliumlectinexpressionandregulationinthemaintenanceofrumenhomeostasis
_version_	1718394428388802560

Diet-induced reconstruction of mucosal microbiota associated with alterations of epithelium lectin expression and regulation in the maintenance of rumen homeostasis

Ejemplares similares