A conserved immunogenic and vulnerable site on the coronavirus spike protein delineated by cross-reactive monoclonal antibodies

Here, the authors report the isolation and characterization of two human monoclonal antibodies (mAbs) from immunized mice with trimeric spike ectodomains of three human betacoronaviruses HCoV-OC43, SARS-CoV and MERSCoV, and show that while exhibiting cross-reactivity, the mAbs only neutralize MERS-C...

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Autores principales:	Chunyan Wang, Rien van Haperen, Javier Gutiérrez-Álvarez, Wentao Li, Nisreen M. A. Okba, Irina Albulescu, Ivy Widjaja, Brenda van Dieren, Raul Fernandez-Delgado, Isabel Sola, Daniel L. Hurdiss, Olalekan Daramola, Frank Grosveld, Frank J. M. van Kuppeveld, Bart L. Haagmans, Luis Enjuanes, Dubravka Drabek, Berend-Jan Bosch
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/db6da7f5d9bb4e1c916d69bc00d5ab39
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spelling	oai:doaj.org-article:db6da7f5d9bb4e1c916d69bc00d5ab392021-12-02T13:17:39ZA conserved immunogenic and vulnerable site on the coronavirus spike protein delineated by cross-reactive monoclonal antibodies10.1038/s41467-021-21968-w2041-1723https://doaj.org/article/db6da7f5d9bb4e1c916d69bc00d5ab392021-03-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-21968-whttps://doaj.org/toc/2041-1723Here, the authors report the isolation and characterization of two human monoclonal antibodies (mAbs) from immunized mice with trimeric spike ectodomains of three human betacoronaviruses HCoV-OC43, SARS-CoV and MERSCoV, and show that while exhibiting cross-reactivity, the mAbs only neutralize MERS-CoV but not SARS-CoV nor SARS-CoV-2, likely due to the subtle epitope differences in the spike S2 fusion subunit.Chunyan WangRien van HaperenJavier Gutiérrez-ÁlvarezWentao LiNisreen M. A. OkbaIrina AlbulescuIvy WidjajaBrenda van DierenRaul Fernandez-DelgadoIsabel SolaDaniel L. HurdissOlalekan DaramolaFrank GrosveldFrank J. M. van KuppeveldBart L. HaagmansLuis EnjuanesDubravka DrabekBerend-Jan BoschNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Chunyan Wang Rien van Haperen Javier Gutiérrez-Álvarez Wentao Li Nisreen M. A. Okba Irina Albulescu Ivy Widjaja Brenda van Dieren Raul Fernandez-Delgado Isabel Sola Daniel L. Hurdiss Olalekan Daramola Frank Grosveld Frank J. M. van Kuppeveld Bart L. Haagmans Luis Enjuanes Dubravka Drabek Berend-Jan Bosch A conserved immunogenic and vulnerable site on the coronavirus spike protein delineated by cross-reactive monoclonal antibodies
description	Here, the authors report the isolation and characterization of two human monoclonal antibodies (mAbs) from immunized mice with trimeric spike ectodomains of three human betacoronaviruses HCoV-OC43, SARS-CoV and MERSCoV, and show that while exhibiting cross-reactivity, the mAbs only neutralize MERS-CoV but not SARS-CoV nor SARS-CoV-2, likely due to the subtle epitope differences in the spike S2 fusion subunit.
format	article
author	Chunyan Wang Rien van Haperen Javier Gutiérrez-Álvarez Wentao Li Nisreen M. A. Okba Irina Albulescu Ivy Widjaja Brenda van Dieren Raul Fernandez-Delgado Isabel Sola Daniel L. Hurdiss Olalekan Daramola Frank Grosveld Frank J. M. van Kuppeveld Bart L. Haagmans Luis Enjuanes Dubravka Drabek Berend-Jan Bosch
author_facet	Chunyan Wang Rien van Haperen Javier Gutiérrez-Álvarez Wentao Li Nisreen M. A. Okba Irina Albulescu Ivy Widjaja Brenda van Dieren Raul Fernandez-Delgado Isabel Sola Daniel L. Hurdiss Olalekan Daramola Frank Grosveld Frank J. M. van Kuppeveld Bart L. Haagmans Luis Enjuanes Dubravka Drabek Berend-Jan Bosch
author_sort	Chunyan Wang
title	A conserved immunogenic and vulnerable site on the coronavirus spike protein delineated by cross-reactive monoclonal antibodies
title_short	A conserved immunogenic and vulnerable site on the coronavirus spike protein delineated by cross-reactive monoclonal antibodies
title_full	A conserved immunogenic and vulnerable site on the coronavirus spike protein delineated by cross-reactive monoclonal antibodies
title_fullStr	A conserved immunogenic and vulnerable site on the coronavirus spike protein delineated by cross-reactive monoclonal antibodies
title_full_unstemmed	A conserved immunogenic and vulnerable site on the coronavirus spike protein delineated by cross-reactive monoclonal antibodies
title_sort	conserved immunogenic and vulnerable site on the coronavirus spike protein delineated by cross-reactive monoclonal antibodies
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/db6da7f5d9bb4e1c916d69bc00d5ab39
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A conserved immunogenic and vulnerable site on the coronavirus spike protein delineated by cross-reactive monoclonal antibodies

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