Identification and analysis of circulating long non-coding RNAs with high significance in diabetic cardiomyopathy

Abstract Diabetic cardiomyopathy (DCM) lacks diagnostic biomarkers. Circulating long non-coding RNAs (lncRNAs) can serve as valuable diagnostic biomarkers in cardiovascular disease. To seek potential lncRNAs as a diagnostic biomarker for DCM, we investigated the genome-wide expression profiling of c...

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Autores principales:	Tarun Pant, Anuradha Dhanasekaran, Ming Zhao, Edward B. Thorp, Joseph M. Forbess, Zeljko J. Bosnjak, Ivor J. Benjamin, Zhi-Dong Ge
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/db775967c0f041a5b9b8b63d60e560b5
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spelling	oai:doaj.org-article:db775967c0f041a5b9b8b63d60e560b52021-12-02T13:57:48ZIdentification and analysis of circulating long non-coding RNAs with high significance in diabetic cardiomyopathy10.1038/s41598-021-82345-72045-2322https://doaj.org/article/db775967c0f041a5b9b8b63d60e560b52021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-82345-7https://doaj.org/toc/2045-2322Abstract Diabetic cardiomyopathy (DCM) lacks diagnostic biomarkers. Circulating long non-coding RNAs (lncRNAs) can serve as valuable diagnostic biomarkers in cardiovascular disease. To seek potential lncRNAs as a diagnostic biomarker for DCM, we investigated the genome-wide expression profiling of circulating lncRNAs and mRNAs in type 2 diabetic db/db mice with and without DCM and performed bioinformatic analyses of the deregulated lncRNA-mRNA co-expression network. Db/db mice had obesity and hyperglycemia with normal cardiac function at 6 weeks of age (diabetes without DCM) but with an impaired cardiac function at 20 weeks of age (DCM) on an isolated Langendorff apparatus. Compared with the age-matched controls, 152 circulating lncRNAs, 127 mRNAs and 3355 lncRNAs, 2580 mRNAs were deregulated in db/db mice without and with DCM, respectively. The lncRNA-mRNA co-expression network analysis showed that five deregulated lncRNAs, XLOC015617, AK035192, Gm10435, TCR-α chain, and MouselincRNA0135, have the maximum connections with differentially expressed mRNAs. Bioinformatic analysis revealed that these five lncRNAs were highly associated with the development and motion of myofilaments, regulation of inflammatory and immune responses, and apoptosis. This finding was validated by the ultrastructural examination of myocardial samples from the db/db mice with DCM using electron microscopy and changes in the expression of myocardial tumor necrosis factor-α and phosphorylated p38 mitogen-activated protein kinase in db/db mice with DCM. These results indicate that XLOC015617, AK035192, Gm10435, TCR-α chain, and MouselincRNA0135 are crucial circulating lncRNAs in the pathogenesis of DCM. These five circulating lncRNAs may have high potential as a diagnostic biomarker for DCM.Tarun PantAnuradha DhanasekaranMing ZhaoEdward B. ThorpJoseph M. ForbessZeljko J. BosnjakIvor J. BenjaminZhi-Dong GeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tarun Pant Anuradha Dhanasekaran Ming Zhao Edward B. Thorp Joseph M. Forbess Zeljko J. Bosnjak Ivor J. Benjamin Zhi-Dong Ge Identification and analysis of circulating long non-coding RNAs with high significance in diabetic cardiomyopathy
description	Abstract Diabetic cardiomyopathy (DCM) lacks diagnostic biomarkers. Circulating long non-coding RNAs (lncRNAs) can serve as valuable diagnostic biomarkers in cardiovascular disease. To seek potential lncRNAs as a diagnostic biomarker for DCM, we investigated the genome-wide expression profiling of circulating lncRNAs and mRNAs in type 2 diabetic db/db mice with and without DCM and performed bioinformatic analyses of the deregulated lncRNA-mRNA co-expression network. Db/db mice had obesity and hyperglycemia with normal cardiac function at 6 weeks of age (diabetes without DCM) but with an impaired cardiac function at 20 weeks of age (DCM) on an isolated Langendorff apparatus. Compared with the age-matched controls, 152 circulating lncRNAs, 127 mRNAs and 3355 lncRNAs, 2580 mRNAs were deregulated in db/db mice without and with DCM, respectively. The lncRNA-mRNA co-expression network analysis showed that five deregulated lncRNAs, XLOC015617, AK035192, Gm10435, TCR-α chain, and MouselincRNA0135, have the maximum connections with differentially expressed mRNAs. Bioinformatic analysis revealed that these five lncRNAs were highly associated with the development and motion of myofilaments, regulation of inflammatory and immune responses, and apoptosis. This finding was validated by the ultrastructural examination of myocardial samples from the db/db mice with DCM using electron microscopy and changes in the expression of myocardial tumor necrosis factor-α and phosphorylated p38 mitogen-activated protein kinase in db/db mice with DCM. These results indicate that XLOC015617, AK035192, Gm10435, TCR-α chain, and MouselincRNA0135 are crucial circulating lncRNAs in the pathogenesis of DCM. These five circulating lncRNAs may have high potential as a diagnostic biomarker for DCM.
format	article
author	Tarun Pant Anuradha Dhanasekaran Ming Zhao Edward B. Thorp Joseph M. Forbess Zeljko J. Bosnjak Ivor J. Benjamin Zhi-Dong Ge
author_facet	Tarun Pant Anuradha Dhanasekaran Ming Zhao Edward B. Thorp Joseph M. Forbess Zeljko J. Bosnjak Ivor J. Benjamin Zhi-Dong Ge
author_sort	Tarun Pant
title	Identification and analysis of circulating long non-coding RNAs with high significance in diabetic cardiomyopathy
title_short	Identification and analysis of circulating long non-coding RNAs with high significance in diabetic cardiomyopathy
title_full	Identification and analysis of circulating long non-coding RNAs with high significance in diabetic cardiomyopathy
title_fullStr	Identification and analysis of circulating long non-coding RNAs with high significance in diabetic cardiomyopathy
title_full_unstemmed	Identification and analysis of circulating long non-coding RNAs with high significance in diabetic cardiomyopathy
title_sort	identification and analysis of circulating long non-coding rnas with high significance in diabetic cardiomyopathy
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/db775967c0f041a5b9b8b63d60e560b5
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Identification and analysis of circulating long non-coding RNAs with high significance in diabetic cardiomyopathy

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