The Microdamage and Expression of Sclerostin in Peri-implant Bone under One-time Shock Force Generated by Impact

Abstract Osseointegration is the key to implant stability and occlusal support. Biomechanical response and remodeling of peri-implant bone occurs under impact loading. Sclerostin participates in bone formation and resorption through Wnt and RANKL pathways. However the mechanism of microdamage and ex...

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Autores principales:	Xiaoou Diao, Zhirui Li, Baili An, Haitao Xin, Yulu Wu, Kai Li, Fan Feng, Chenyun Dou
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/db7b78cbb1004948b742b34f138e1239
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spelling	oai:doaj.org-article:db7b78cbb1004948b742b34f138e12392021-12-02T11:53:11ZThe Microdamage and Expression of Sclerostin in Peri-implant Bone under One-time Shock Force Generated by Impact10.1038/s41598-017-06867-92045-2322https://doaj.org/article/db7b78cbb1004948b742b34f138e12392017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06867-9https://doaj.org/toc/2045-2322Abstract Osseointegration is the key to implant stability and occlusal support. Biomechanical response and remodeling of peri-implant bone occurs under impact loading. Sclerostin participates in bone formation and resorption through Wnt and RANKL pathways. However the mechanism of microdamage and expression of sclerostin in peri-implant bone under impact load is still unclear. In present study, specific impact forces were applied to the implants with favorable osseointegration in rabbits. The microdamage of peri-implant bone and the expression of sclerostin, β-catenin and RANKL during the process of bone damage and remodeling were investigated by micro-CT, histology, immunofluorescence and RT-qPCR analysis. Interface separation and trabecular fracture were found histologically, which were consistent with micro-CT analyses. Throughout remodeling, bone resorption was observed during the first 14 days after impact, and osseointegration and normal trabecular structure were found by 28 d. The expression of sclerostin and RANKL increased after impact and reached a maximum by 14 d, then decreased gradually to normal levels by 28 d. And β-catenin expression was opposite. Results indicated that sclerostin may involve in the peri-implant bone damage caused by impact and remodeling through Wnt/β-catenin and RANKL/RANK pathways. It will provide a new insight in the diagnosis and treatment for patients suffering impact.Xiaoou DiaoZhirui LiBaili AnHaitao XinYulu WuKai LiFan FengChenyun DouNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xiaoou Diao Zhirui Li Baili An Haitao Xin Yulu Wu Kai Li Fan Feng Chenyun Dou The Microdamage and Expression of Sclerostin in Peri-implant Bone under One-time Shock Force Generated by Impact
description	Abstract Osseointegration is the key to implant stability and occlusal support. Biomechanical response and remodeling of peri-implant bone occurs under impact loading. Sclerostin participates in bone formation and resorption through Wnt and RANKL pathways. However the mechanism of microdamage and expression of sclerostin in peri-implant bone under impact load is still unclear. In present study, specific impact forces were applied to the implants with favorable osseointegration in rabbits. The microdamage of peri-implant bone and the expression of sclerostin, β-catenin and RANKL during the process of bone damage and remodeling were investigated by micro-CT, histology, immunofluorescence and RT-qPCR analysis. Interface separation and trabecular fracture were found histologically, which were consistent with micro-CT analyses. Throughout remodeling, bone resorption was observed during the first 14 days after impact, and osseointegration and normal trabecular structure were found by 28 d. The expression of sclerostin and RANKL increased after impact and reached a maximum by 14 d, then decreased gradually to normal levels by 28 d. And β-catenin expression was opposite. Results indicated that sclerostin may involve in the peri-implant bone damage caused by impact and remodeling through Wnt/β-catenin and RANKL/RANK pathways. It will provide a new insight in the diagnosis and treatment for patients suffering impact.
format	article
author	Xiaoou Diao Zhirui Li Baili An Haitao Xin Yulu Wu Kai Li Fan Feng Chenyun Dou
author_facet	Xiaoou Diao Zhirui Li Baili An Haitao Xin Yulu Wu Kai Li Fan Feng Chenyun Dou
author_sort	Xiaoou Diao
title	The Microdamage and Expression of Sclerostin in Peri-implant Bone under One-time Shock Force Generated by Impact
title_short	The Microdamage and Expression of Sclerostin in Peri-implant Bone under One-time Shock Force Generated by Impact
title_full	The Microdamage and Expression of Sclerostin in Peri-implant Bone under One-time Shock Force Generated by Impact
title_fullStr	The Microdamage and Expression of Sclerostin in Peri-implant Bone under One-time Shock Force Generated by Impact
title_full_unstemmed	The Microdamage and Expression of Sclerostin in Peri-implant Bone under One-time Shock Force Generated by Impact
title_sort	microdamage and expression of sclerostin in peri-implant bone under one-time shock force generated by impact
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/db7b78cbb1004948b742b34f138e1239
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The Microdamage and Expression of Sclerostin in Peri-implant Bone under One-time Shock Force Generated by Impact

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