miR-299-3p suppresses cell progression and induces apoptosis by downregulating PAX3 in gastric cancer

Gastric cancer (GC) is ranked the fourth leading cause of cancer-related death, with an over 75% mortality rate worldwide. In recent years, miR-299-3p has been identified as a biomarker in multiple cancers, such as acute promyelocytic leukemia, thyroid cancer, and lung cancer. However, the regulator...

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Autores principales:	Wang Zhenfen, Liu Qing, Huang Ping, Cai Guohao
Formato:	article
Lenguaje:	EN
Publicado:	De Gruyter 2021
Materias:	mir-299-3p pax3 gastric cancer gc biomarkers gc therapy Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/db7eeb1c2e1741b896d847ce81510c5f
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spelling	oai:doaj.org-article:db7eeb1c2e1741b896d847ce81510c5f2021-12-05T14:10:40ZmiR-299-3p suppresses cell progression and induces apoptosis by downregulating PAX3 in gastric cancer2391-541210.1515/biol-2021-0022https://doaj.org/article/db7eeb1c2e1741b896d847ce81510c5f2021-03-01T00:00:00Zhttps://doi.org/10.1515/biol-2021-0022https://doaj.org/toc/2391-5412Gastric cancer (GC) is ranked the fourth leading cause of cancer-related death, with an over 75% mortality rate worldwide. In recent years, miR-299-3p has been identified as a biomarker in multiple cancers, such as acute promyelocytic leukemia, thyroid cancer, and lung cancer. However, the regulatory mechanism of miR-299-3p in GC cell progression is still largely unclear. Cell viability and apoptosis tests were performed by CCK8 and flow cytometry assay, respectively. Transwell assay was recruited to examine cell invasion ability. The interaction between miR-299-3p and PAX3 was determined by the luciferase reporter system. PAX3 protein level was evaluated by western blot assay. The expression of miR-299-3p was downregulated in GC tissues and cell lines (MKN-45, AGS, and MGC-803) compared with the normal tissues and cells. Besides, overexpression of miR-299-3p significantly suppressed proliferation and invasion and promoted apoptosis in GC. Next, we clarified that PAX3 expression was regulated by miR-299-3p using a luciferase reporter system, qRT-PCR, and western blot assay. Additionally, downregulation of PAX3 repressed GC cell progression. The rescue experiments indicated that restoration of PAX3 inversed miR-299-3p-mediated inhibition on cell proliferation and invasion. miR-299-3p suppresses cell proliferation and invasion as well as induces apoptosis by regulating PAX3 expression in GC, representing desirable biomarkers for GC diagnosis and therapy.Wang ZhenfenLiu QingHuang PingCai GuohaoDe Gruyterarticlemir-299-3ppax3gastric cancergc biomarkersgc therapyBiology (General)QH301-705.5ENOpen Life Sciences, Vol 16, Iss 1, Pp 266-276 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	mir-299-3p pax3 gastric cancer gc biomarkers gc therapy Biology (General) QH301-705.5
spellingShingle	mir-299-3p pax3 gastric cancer gc biomarkers gc therapy Biology (General) QH301-705.5 Wang Zhenfen Liu Qing Huang Ping Cai Guohao miR-299-3p suppresses cell progression and induces apoptosis by downregulating PAX3 in gastric cancer
description	Gastric cancer (GC) is ranked the fourth leading cause of cancer-related death, with an over 75% mortality rate worldwide. In recent years, miR-299-3p has been identified as a biomarker in multiple cancers, such as acute promyelocytic leukemia, thyroid cancer, and lung cancer. However, the regulatory mechanism of miR-299-3p in GC cell progression is still largely unclear. Cell viability and apoptosis tests were performed by CCK8 and flow cytometry assay, respectively. Transwell assay was recruited to examine cell invasion ability. The interaction between miR-299-3p and PAX3 was determined by the luciferase reporter system. PAX3 protein level was evaluated by western blot assay. The expression of miR-299-3p was downregulated in GC tissues and cell lines (MKN-45, AGS, and MGC-803) compared with the normal tissues and cells. Besides, overexpression of miR-299-3p significantly suppressed proliferation and invasion and promoted apoptosis in GC. Next, we clarified that PAX3 expression was regulated by miR-299-3p using a luciferase reporter system, qRT-PCR, and western blot assay. Additionally, downregulation of PAX3 repressed GC cell progression. The rescue experiments indicated that restoration of PAX3 inversed miR-299-3p-mediated inhibition on cell proliferation and invasion. miR-299-3p suppresses cell proliferation and invasion as well as induces apoptosis by regulating PAX3 expression in GC, representing desirable biomarkers for GC diagnosis and therapy.
format	article
author	Wang Zhenfen Liu Qing Huang Ping Cai Guohao
author_facet	Wang Zhenfen Liu Qing Huang Ping Cai Guohao
author_sort	Wang Zhenfen
title	miR-299-3p suppresses cell progression and induces apoptosis by downregulating PAX3 in gastric cancer
title_short	miR-299-3p suppresses cell progression and induces apoptosis by downregulating PAX3 in gastric cancer
title_full	miR-299-3p suppresses cell progression and induces apoptosis by downregulating PAX3 in gastric cancer
title_fullStr	miR-299-3p suppresses cell progression and induces apoptosis by downregulating PAX3 in gastric cancer
title_full_unstemmed	miR-299-3p suppresses cell progression and induces apoptosis by downregulating PAX3 in gastric cancer
title_sort	mir-299-3p suppresses cell progression and induces apoptosis by downregulating pax3 in gastric cancer
publisher	De Gruyter
publishDate	2021
url	https://doaj.org/article/db7eeb1c2e1741b896d847ce81510c5f
work_keys_str_mv	AT wangzhenfen mir2993psuppressescellprogressionandinducesapoptosisbydownregulatingpax3ingastriccancer AT liuqing mir2993psuppressescellprogressionandinducesapoptosisbydownregulatingpax3ingastriccancer AT huangping mir2993psuppressescellprogressionandinducesapoptosisbydownregulatingpax3ingastriccancer AT caiguohao mir2993psuppressescellprogressionandinducesapoptosisbydownregulatingpax3ingastriccancer
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miR-299-3p suppresses cell progression and induces apoptosis by downregulating PAX3 in gastric cancer

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