Rabphilin silencing causes dilated cardiomyopathy in a Drosophila model of nephrocyte damage

Abstract Heart failure (HF) and the development of chronic kidney disease (CKD) have a direct association. Both can be cause and consequence of the other. Many factors are known, such as diabetes or hypertension, which can lead to the appearance and/or development of these two conditions. However, i...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Estela Selma-Soriano, Carlos Casillas-Serra, Rubén Artero, Beatriz Llamusi, Juan Antonio Navarro, Josep Redón
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/db828bf2c4714b54b3f66373160ac357
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:db828bf2c4714b54b3f66373160ac357
record_format	dspace
spelling	oai:doaj.org-article:db828bf2c4714b54b3f66373160ac3572021-12-02T18:47:06ZRabphilin silencing causes dilated cardiomyopathy in a Drosophila model of nephrocyte damage10.1038/s41598-021-94710-72045-2322https://doaj.org/article/db828bf2c4714b54b3f66373160ac3572021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-94710-7https://doaj.org/toc/2045-2322Abstract Heart failure (HF) and the development of chronic kidney disease (CKD) have a direct association. Both can be cause and consequence of the other. Many factors are known, such as diabetes or hypertension, which can lead to the appearance and/or development of these two conditions. However, it is suspected that other factors, namely genetic ones, may explain the differences in the manifestation and progression of HF and CKD among patients. One candidate factor is Rph, a gene expressed in the nervous and excretory system in mammals and Drosophila, encoding a Rab small GTPase family effector protein implicated in vesicular trafficking. We found that Rph is expressed in the Drosophila heart, and the silencing of Rph gene expression in this organ had a strong impact in the organization of fibers and functional cardiac parameters. Specifically, we observed a significant increase in diastolic and systolic diameters of the heart tube, which is a phenotype that resembles dilated cardiomyopathy in humans. Importantly, we also show that silencing of Rabphilin (Rph) expression exclusively in the pericardial nephrocytes, which are part of the flies' excretory system, brings about a non-cell-autonomous effect on the Drosophila cardiac system. In summary, in this work, we demonstrate the importance of Rph in the fly cardiac system and how silencing Rph expression in nephrocytes affects the Drosophila cardiac system.Estela Selma-SorianoCarlos Casillas-SerraRubén ArteroBeatriz LlamusiJuan Antonio NavarroJosep RedónNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Estela Selma-Soriano Carlos Casillas-Serra Rubén Artero Beatriz Llamusi Juan Antonio Navarro Josep Redón Rabphilin silencing causes dilated cardiomyopathy in a Drosophila model of nephrocyte damage
description	Abstract Heart failure (HF) and the development of chronic kidney disease (CKD) have a direct association. Both can be cause and consequence of the other. Many factors are known, such as diabetes or hypertension, which can lead to the appearance and/or development of these two conditions. However, it is suspected that other factors, namely genetic ones, may explain the differences in the manifestation and progression of HF and CKD among patients. One candidate factor is Rph, a gene expressed in the nervous and excretory system in mammals and Drosophila, encoding a Rab small GTPase family effector protein implicated in vesicular trafficking. We found that Rph is expressed in the Drosophila heart, and the silencing of Rph gene expression in this organ had a strong impact in the organization of fibers and functional cardiac parameters. Specifically, we observed a significant increase in diastolic and systolic diameters of the heart tube, which is a phenotype that resembles dilated cardiomyopathy in humans. Importantly, we also show that silencing of Rabphilin (Rph) expression exclusively in the pericardial nephrocytes, which are part of the flies' excretory system, brings about a non-cell-autonomous effect on the Drosophila cardiac system. In summary, in this work, we demonstrate the importance of Rph in the fly cardiac system and how silencing Rph expression in nephrocytes affects the Drosophila cardiac system.
format	article
author	Estela Selma-Soriano Carlos Casillas-Serra Rubén Artero Beatriz Llamusi Juan Antonio Navarro Josep Redón
author_facet	Estela Selma-Soriano Carlos Casillas-Serra Rubén Artero Beatriz Llamusi Juan Antonio Navarro Josep Redón
author_sort	Estela Selma-Soriano
title	Rabphilin silencing causes dilated cardiomyopathy in a Drosophila model of nephrocyte damage
title_short	Rabphilin silencing causes dilated cardiomyopathy in a Drosophila model of nephrocyte damage
title_full	Rabphilin silencing causes dilated cardiomyopathy in a Drosophila model of nephrocyte damage
title_fullStr	Rabphilin silencing causes dilated cardiomyopathy in a Drosophila model of nephrocyte damage
title_full_unstemmed	Rabphilin silencing causes dilated cardiomyopathy in a Drosophila model of nephrocyte damage
title_sort	rabphilin silencing causes dilated cardiomyopathy in a drosophila model of nephrocyte damage
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/db828bf2c4714b54b3f66373160ac357
work_keys_str_mv	AT estelaselmasoriano rabphilinsilencingcausesdilatedcardiomyopathyinadrosophilamodelofnephrocytedamage AT carloscasillasserra rabphilinsilencingcausesdilatedcardiomyopathyinadrosophilamodelofnephrocytedamage AT rubenartero rabphilinsilencingcausesdilatedcardiomyopathyinadrosophilamodelofnephrocytedamage AT beatrizllamusi rabphilinsilencingcausesdilatedcardiomyopathyinadrosophilamodelofnephrocytedamage AT juanantonionavarro rabphilinsilencingcausesdilatedcardiomyopathyinadrosophilamodelofnephrocytedamage AT josepredon rabphilinsilencingcausesdilatedcardiomyopathyinadrosophilamodelofnephrocytedamage
_version_	1718377710629158912

Rabphilin silencing causes dilated cardiomyopathy in a Drosophila model of nephrocyte damage

Ejemplares similares