In vitro and in vivo study of a nanoliposomal cisplatin as a radiosensitizer

Xiaomeng Zhang1*, Huanjun Yang1*, Ke Gu1, Jian Chen2, Mengjie Rui2, Guo-Liang Jiang11Departments of Radiation Oncology, Fudan University Shanghai Cancer Center; Department of Oncology, Shanghai Medical College,Fudan University,Shanghai, People’s Republic of China; 2School of Pharmacy,...

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Autores principales: Xiaomeng Zhang, Huanjun Yang, Ke Gu, et al
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Publicado: Dove Medical Press 2011
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spelling oai:doaj.org-article:db8a36fcc87d457ab5845336dee7aa8d2021-12-02T02:42:16ZIn vitro and in vivo study of a nanoliposomal cisplatin as a radiosensitizer1176-91141178-2013https://doaj.org/article/db8a36fcc87d457ab5845336dee7aa8d2011-02-01T00:00:00Zhttp://www.dovepress.com/in-vitro-and-in-vivo-study-of-a-nanoliposomal-cisplatin-as-a-radiosens-a6411https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Xiaomeng Zhang1*, Huanjun Yang1*, Ke Gu1, Jian Chen2, Mengjie Rui2, Guo-Liang Jiang11Departments of Radiation Oncology, Fudan University Shanghai Cancer Center; Department of Oncology, Shanghai Medical College,Fudan University,Shanghai, People’s Republic of China; 2School of Pharmacy, Shanghai Jiao Tong University, Shanghai, People’s Republic of China; *Xiaomeng Zhang and Huanjun Yang share the first authorshipObjective: To investigate the in vitro and in vivo radiosensitization effect of an institutionally designed nanoliposome encapsulated cisplatin (NLE-CDDP).Materials and methods: NLE-CDDP was developed by our institute. In vitro radiosensitization of NLE-CDDP was evaluated by colony forming assay in A549 cells. In vivo radiosensitization was studied with tumor growth delay (TGD) in Lewis lung carcinoma. The radiosensitization for normal tissue was investigated by jejunal crypt survival. The radiosensitization studies were carried out with a 72 h interval between drug administration and irradiation. The mice were treated with 6 mg/kg of NLE-CDDP or CDDP followed by single doses of 2 Gy, 6 Gy, 16 Gy, and 28 Gy. Sensitization enhancement ratio (SER) was calculated by D0s of cell survival curves for A549 cells, doses needed to yield TGD of 20 days in Lewis lung carcinoma, or D0s of survival curves in crypt cells in radiation alone and radiation plus drug groups.Results: Our NLE-CDDP could inhibit A549 cells in vitro with half maximal inhibitory concentration of 1.12 µg/mL, and its toxicity was 2.35 times that observed in CDDP. For in vitro studies of A549 cells, SERs of NLE-CDDP and CDDP were 1.40 and 1.14, respectively, when combined with irradiation. For in vivo studies of Lewis lung carcinoma, the strongest radiosensitization was found in the 72 h interval between NLE-CDDP and irradiation. When given 72 h prior to irradiation, NLE-CDDP yielded higher radiosensitization than CDDP (SER of 4.92 vs 3.21) and slightly increased injury in jejunal crypt cells (SER of 1.15 vs 1.19). Therefore, NLE-CDDP resulted in a higher TGF than did CDDP (4.28 vs 2.70) when SERs were compared between experiments in vivo and in jejunal crypt cell studies.Conclusions: Our NLE-CDDP was demonstrated to have radiosensitization with TGF of 4.28 when administrated 72 h prior to irradiation.Keywords: cisplatin, liposome, nanoparticle, radiation, radiosensitization  Xiaomeng ZhangHuanjun YangKe Guet alDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2011, Iss default, Pp 437-444 (2011)
institution DOAJ
collection DOAJ
language EN
topic Medicine (General)
R5-920
spellingShingle Medicine (General)
R5-920
Xiaomeng Zhang
Huanjun Yang
Ke Gu
et al
In vitro and in vivo study of a nanoliposomal cisplatin as a radiosensitizer
description Xiaomeng Zhang1*, Huanjun Yang1*, Ke Gu1, Jian Chen2, Mengjie Rui2, Guo-Liang Jiang11Departments of Radiation Oncology, Fudan University Shanghai Cancer Center; Department of Oncology, Shanghai Medical College,Fudan University,Shanghai, People’s Republic of China; 2School of Pharmacy, Shanghai Jiao Tong University, Shanghai, People’s Republic of China; *Xiaomeng Zhang and Huanjun Yang share the first authorshipObjective: To investigate the in vitro and in vivo radiosensitization effect of an institutionally designed nanoliposome encapsulated cisplatin (NLE-CDDP).Materials and methods: NLE-CDDP was developed by our institute. In vitro radiosensitization of NLE-CDDP was evaluated by colony forming assay in A549 cells. In vivo radiosensitization was studied with tumor growth delay (TGD) in Lewis lung carcinoma. The radiosensitization for normal tissue was investigated by jejunal crypt survival. The radiosensitization studies were carried out with a 72 h interval between drug administration and irradiation. The mice were treated with 6 mg/kg of NLE-CDDP or CDDP followed by single doses of 2 Gy, 6 Gy, 16 Gy, and 28 Gy. Sensitization enhancement ratio (SER) was calculated by D0s of cell survival curves for A549 cells, doses needed to yield TGD of 20 days in Lewis lung carcinoma, or D0s of survival curves in crypt cells in radiation alone and radiation plus drug groups.Results: Our NLE-CDDP could inhibit A549 cells in vitro with half maximal inhibitory concentration of 1.12 µg/mL, and its toxicity was 2.35 times that observed in CDDP. For in vitro studies of A549 cells, SERs of NLE-CDDP and CDDP were 1.40 and 1.14, respectively, when combined with irradiation. For in vivo studies of Lewis lung carcinoma, the strongest radiosensitization was found in the 72 h interval between NLE-CDDP and irradiation. When given 72 h prior to irradiation, NLE-CDDP yielded higher radiosensitization than CDDP (SER of 4.92 vs 3.21) and slightly increased injury in jejunal crypt cells (SER of 1.15 vs 1.19). Therefore, NLE-CDDP resulted in a higher TGF than did CDDP (4.28 vs 2.70) when SERs were compared between experiments in vivo and in jejunal crypt cell studies.Conclusions: Our NLE-CDDP was demonstrated to have radiosensitization with TGF of 4.28 when administrated 72 h prior to irradiation.Keywords: cisplatin, liposome, nanoparticle, radiation, radiosensitization 
format article
author Xiaomeng Zhang
Huanjun Yang
Ke Gu
et al
author_facet Xiaomeng Zhang
Huanjun Yang
Ke Gu
et al
author_sort Xiaomeng Zhang
title In vitro and in vivo study of a nanoliposomal cisplatin as a radiosensitizer
title_short In vitro and in vivo study of a nanoliposomal cisplatin as a radiosensitizer
title_full In vitro and in vivo study of a nanoliposomal cisplatin as a radiosensitizer
title_fullStr In vitro and in vivo study of a nanoliposomal cisplatin as a radiosensitizer
title_full_unstemmed In vitro and in vivo study of a nanoliposomal cisplatin as a radiosensitizer
title_sort in vitro and in vivo study of a nanoliposomal cisplatin as a radiosensitizer
publisher Dove Medical Press
publishDate 2011
url https://doaj.org/article/db8a36fcc87d457ab5845336dee7aa8d
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AT huanjunyang invitroandinvivostudyofananoliposomalcisplatinasaradiosensitizer
AT kegu invitroandinvivostudyofananoliposomalcisplatinasaradiosensitizer
AT etal invitroandinvivostudyofananoliposomalcisplatinasaradiosensitizer
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