Ultra-weak photon emission as a dynamic tool for monitoring oxidative stress metabolism

Abstract In recent years, excessive oxidative metabolism has been reported as a critical determinant of pathogenicity in many diseases. The advent of a simple tool that can provide a physiological readout of oxidative stress would be a major step towards monitoring this dynamic process in biological...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Rosilene Cristina Rossetto Burgos, Johannes Cornelius Schoeman, Lennart Jan van Winden, Kateřina Červinková, Rawi Ramautar, Eduard P. A. Van Wijk, Michal Cifra, Ruud Berger, Thomas Hankemeier, Jan van der Greef
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/db8be495d5d644be8296f1232c177b2e
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:db8be495d5d644be8296f1232c177b2e
record_format	dspace
spelling	oai:doaj.org-article:db8be495d5d644be8296f1232c177b2e2021-12-02T15:05:44ZUltra-weak photon emission as a dynamic tool for monitoring oxidative stress metabolism10.1038/s41598-017-01229-x2045-2322https://doaj.org/article/db8be495d5d644be8296f1232c177b2e2017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01229-xhttps://doaj.org/toc/2045-2322Abstract In recent years, excessive oxidative metabolism has been reported as a critical determinant of pathogenicity in many diseases. The advent of a simple tool that can provide a physiological readout of oxidative stress would be a major step towards monitoring this dynamic process in biological systems, while also improving our understanding of this process. Ultra-weak photon emission (UPE) has been proposed as a potential tool for measuring oxidative processes due to the association between UPE and reactive oxygen species. Here, we used HL-60 cells as an in vitro model to test the potential of using UPE as readout for dynamically monitoring oxidative stress after inducing respiratory burst. In addition, to probe for possible changes in oxidative metabolism, we performed targeted metabolomics on cell extracts and culture medium. Lastly, we tested the effects of treating cells with the NADPH oxidase inhibitor diphenyleneiodonium chloride (DPI). Our results show that UPE can be used as readout for measuring oxidative stress metabolism and related processes.Rosilene Cristina Rossetto BurgosJohannes Cornelius SchoemanLennart Jan van WindenKateřina ČervinkováRawi RamautarEduard P. A. Van WijkMichal CifraRuud BergerThomas HankemeierJan van der GreefNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Rosilene Cristina Rossetto Burgos Johannes Cornelius Schoeman Lennart Jan van Winden Kateřina Červinková Rawi Ramautar Eduard P. A. Van Wijk Michal Cifra Ruud Berger Thomas Hankemeier Jan van der Greef Ultra-weak photon emission as a dynamic tool for monitoring oxidative stress metabolism
description	Abstract In recent years, excessive oxidative metabolism has been reported as a critical determinant of pathogenicity in many diseases. The advent of a simple tool that can provide a physiological readout of oxidative stress would be a major step towards monitoring this dynamic process in biological systems, while also improving our understanding of this process. Ultra-weak photon emission (UPE) has been proposed as a potential tool for measuring oxidative processes due to the association between UPE and reactive oxygen species. Here, we used HL-60 cells as an in vitro model to test the potential of using UPE as readout for dynamically monitoring oxidative stress after inducing respiratory burst. In addition, to probe for possible changes in oxidative metabolism, we performed targeted metabolomics on cell extracts and culture medium. Lastly, we tested the effects of treating cells with the NADPH oxidase inhibitor diphenyleneiodonium chloride (DPI). Our results show that UPE can be used as readout for measuring oxidative stress metabolism and related processes.
format	article
author	Rosilene Cristina Rossetto Burgos Johannes Cornelius Schoeman Lennart Jan van Winden Kateřina Červinková Rawi Ramautar Eduard P. A. Van Wijk Michal Cifra Ruud Berger Thomas Hankemeier Jan van der Greef
author_facet	Rosilene Cristina Rossetto Burgos Johannes Cornelius Schoeman Lennart Jan van Winden Kateřina Červinková Rawi Ramautar Eduard P. A. Van Wijk Michal Cifra Ruud Berger Thomas Hankemeier Jan van der Greef
author_sort	Rosilene Cristina Rossetto Burgos
title	Ultra-weak photon emission as a dynamic tool for monitoring oxidative stress metabolism
title_short	Ultra-weak photon emission as a dynamic tool for monitoring oxidative stress metabolism
title_full	Ultra-weak photon emission as a dynamic tool for monitoring oxidative stress metabolism
title_fullStr	Ultra-weak photon emission as a dynamic tool for monitoring oxidative stress metabolism
title_full_unstemmed	Ultra-weak photon emission as a dynamic tool for monitoring oxidative stress metabolism
title_sort	ultra-weak photon emission as a dynamic tool for monitoring oxidative stress metabolism
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/db8be495d5d644be8296f1232c177b2e
work_keys_str_mv	AT rosilenecristinarossettoburgos ultraweakphotonemissionasadynamictoolformonitoringoxidativestressmetabolism AT johannescorneliusschoeman ultraweakphotonemissionasadynamictoolformonitoringoxidativestressmetabolism AT lennartjanvanwinden ultraweakphotonemissionasadynamictoolformonitoringoxidativestressmetabolism AT katerinacervinkova ultraweakphotonemissionasadynamictoolformonitoringoxidativestressmetabolism AT rawiramautar ultraweakphotonemissionasadynamictoolformonitoringoxidativestressmetabolism AT eduardpavanwijk ultraweakphotonemissionasadynamictoolformonitoringoxidativestressmetabolism AT michalcifra ultraweakphotonemissionasadynamictoolformonitoringoxidativestressmetabolism AT ruudberger ultraweakphotonemissionasadynamictoolformonitoringoxidativestressmetabolism AT thomashankemeier ultraweakphotonemissionasadynamictoolformonitoringoxidativestressmetabolism AT janvandergreef ultraweakphotonemissionasadynamictoolformonitoringoxidativestressmetabolism
_version_	1718388737806696448

Ultra-weak photon emission as a dynamic tool for monitoring oxidative stress metabolism

Ejemplares similares