The influence of direct laser metal sintering implants on the early stages of osseointegration in diabetic mini-pigs

Naiwen Tan,1–3,* Xiangwei Liu,1,2,* Yanhui Cai,4 Sijia Zhang,1,2 Bo Jian,1,2 Yuchao Zhou,1,2 Xiaoru Xu,1,2 Shuai Ren,1,2 Hongbo Wei,1,2 Yingliang Song1,2 1State Key Laboratory of Military Stomatology, National Clinical Research Center for Oral Diseases, Shaanxi Engineering Research Center...

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Autores principales:	Tan NW, Liu XW, Cai YH, Zhang SJ, Jian B, Zhou YC, Xu XR, Ren S, Wei HB, Song YL
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	laser manufacturing dental implants diabetes mellitus osseointegration Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/db944d72abc7427fa16a7645dd9237e1
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spelling	oai:doaj.org-article:db944d72abc7427fa16a7645dd9237e12021-12-02T02:04:23ZThe influence of direct laser metal sintering implants on the early stages of osseointegration in diabetic mini-pigs1178-2013https://doaj.org/article/db944d72abc7427fa16a7645dd9237e12017-07-01T00:00:00Zhttps://www.dovepress.com/the-influence-of-direct-laser-metal-sintering-implants-on-the-early-st-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Naiwen Tan,1–3,* Xiangwei Liu,1,2,* Yanhui Cai,4 Sijia Zhang,1,2 Bo Jian,1,2 Yuchao Zhou,1,2 Xiaoru Xu,1,2 Shuai Ren,1,2 Hongbo Wei,1,2 Yingliang Song1,2 1State Key Laboratory of Military Stomatology, National Clinical Research Center for Oral Diseases, Shaanxi Engineering Research Center for Dental Materials and Advanced Manufacture, Xi’an, Shaanxi, China; 2Department of Implant Dentistry, School of Stomatology, The Fourth Military Medical University, Xi’an, Shaanxi, China; 3Department of Stomatology, Hospital 463 of PLA, Xi’an, Shaanxi, China; 4Department of Anesthesiology, Xijing Hospital, The Fourth Military Medical University, Xi’an, Shaanxi, China *These authors contributed equally to this work Background: High failure rates of oral implants have been reported in diabetic patients due to the disruption of osseointegration. The aim of this study was to investigate whether direct laser metal sintering (DLMS) could improve osseointegration in diabetic animal models. Methods: Surface characterizations were carried out on two types of implants. Cell morphology and the osteogenic-related gene expression of MG63 cells were observed under conditions of DLMS and microarc oxidation (MAO). A diabetes model in mini-pigs was established by intravenous injection of streptozotocin (150 mg/kg), and a total of 36 implants were inserted into the mandibular region. Micro-computed tomography (micro-CT) and histologic evaluations were performed 3 and 6 months after implantation. Results: The Ra (the average of the absolute height of all points) of MAO surface was 2.3±0.3 µm while the DLMS surface showed the Ra of 27.4±1.1 µm. The cells on DLMS implants spread out more podia than those on MAO implants through cell morphology analysis. Osteogenic-related gene expression was also dramatically increased in the DLMS group. Obvious improvement was observed in the micro-CT and Van Gieson staining analyses of DLMS implants compared with MAO at 3 months, although this difference disappeared by 6 months. DLMS implants showed a higher bone–implant contact percentage (33.2%±11.2%) at 3 months compared with MAO group (18.9%±7.3%) while similar results were showed at 6 months between DLMS group (42.8%±10.1%) and MAO group (38.3%±10.8%). Conclusion: The three-dimensional environment of implant surfaces with highly porous and fully interconnected channel and pore architectures can improve cell spreading and accelerate the progress of osseointegration in diabetic mini-pigs. Keywords: laser manufacturing, dental implants, diabetes mellitus, osseointegrationTan NWLiu XWCai YHZhang SJJian BZhou YCXu XRRen SWei HBSong YLDove Medical Pressarticlelaser manufacturingdental implantsdiabetes mellitusosseointegrationMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 5433-5442 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	laser manufacturing dental implants diabetes mellitus osseointegration Medicine (General) R5-920
spellingShingle	laser manufacturing dental implants diabetes mellitus osseointegration Medicine (General) R5-920 Tan NW Liu XW Cai YH Zhang SJ Jian B Zhou YC Xu XR Ren S Wei HB Song YL The influence of direct laser metal sintering implants on the early stages of osseointegration in diabetic mini-pigs
description	Naiwen Tan,1–3,* Xiangwei Liu,1,2,* Yanhui Cai,4 Sijia Zhang,1,2 Bo Jian,1,2 Yuchao Zhou,1,2 Xiaoru Xu,1,2 Shuai Ren,1,2 Hongbo Wei,1,2 Yingliang Song1,2 1State Key Laboratory of Military Stomatology, National Clinical Research Center for Oral Diseases, Shaanxi Engineering Research Center for Dental Materials and Advanced Manufacture, Xi’an, Shaanxi, China; 2Department of Implant Dentistry, School of Stomatology, The Fourth Military Medical University, Xi’an, Shaanxi, China; 3Department of Stomatology, Hospital 463 of PLA, Xi’an, Shaanxi, China; 4Department of Anesthesiology, Xijing Hospital, The Fourth Military Medical University, Xi’an, Shaanxi, China *These authors contributed equally to this work Background: High failure rates of oral implants have been reported in diabetic patients due to the disruption of osseointegration. The aim of this study was to investigate whether direct laser metal sintering (DLMS) could improve osseointegration in diabetic animal models. Methods: Surface characterizations were carried out on two types of implants. Cell morphology and the osteogenic-related gene expression of MG63 cells were observed under conditions of DLMS and microarc oxidation (MAO). A diabetes model in mini-pigs was established by intravenous injection of streptozotocin (150 mg/kg), and a total of 36 implants were inserted into the mandibular region. Micro-computed tomography (micro-CT) and histologic evaluations were performed 3 and 6 months after implantation. Results: The Ra (the average of the absolute height of all points) of MAO surface was 2.3±0.3 µm while the DLMS surface showed the Ra of 27.4±1.1 µm. The cells on DLMS implants spread out more podia than those on MAO implants through cell morphology analysis. Osteogenic-related gene expression was also dramatically increased in the DLMS group. Obvious improvement was observed in the micro-CT and Van Gieson staining analyses of DLMS implants compared with MAO at 3 months, although this difference disappeared by 6 months. DLMS implants showed a higher bone–implant contact percentage (33.2%±11.2%) at 3 months compared with MAO group (18.9%±7.3%) while similar results were showed at 6 months between DLMS group (42.8%±10.1%) and MAO group (38.3%±10.8%). Conclusion: The three-dimensional environment of implant surfaces with highly porous and fully interconnected channel and pore architectures can improve cell spreading and accelerate the progress of osseointegration in diabetic mini-pigs. Keywords: laser manufacturing, dental implants, diabetes mellitus, osseointegration
format	article
author	Tan NW Liu XW Cai YH Zhang SJ Jian B Zhou YC Xu XR Ren S Wei HB Song YL
author_facet	Tan NW Liu XW Cai YH Zhang SJ Jian B Zhou YC Xu XR Ren S Wei HB Song YL
author_sort	Tan NW
title	The influence of direct laser metal sintering implants on the early stages of osseointegration in diabetic mini-pigs
title_short	The influence of direct laser metal sintering implants on the early stages of osseointegration in diabetic mini-pigs
title_full	The influence of direct laser metal sintering implants on the early stages of osseointegration in diabetic mini-pigs
title_fullStr	The influence of direct laser metal sintering implants on the early stages of osseointegration in diabetic mini-pigs
title_full_unstemmed	The influence of direct laser metal sintering implants on the early stages of osseointegration in diabetic mini-pigs
title_sort	influence of direct laser metal sintering implants on the early stages of osseointegration in diabetic mini-pigs
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/db944d72abc7427fa16a7645dd9237e1
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The influence of direct laser metal sintering implants on the early stages of osseointegration in diabetic mini-pigs

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