Recombinant cystatin-like protein-based competition ELISA for Trichinella spiralis antibody test in multihost sera.

<h4>Objectives</h4>Trichinella spiralis is a zoonotic parasite with a complex parasitic life cycle and exposed to animals or humans by infectious meat. To control transmissions of T. spiralis through the food chain to humans, sensitive and selective multihost sera-diagnosis is urgent nee...

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Autores principales:	Yan Liu, Ning Xu, Yansong Li, Bin Tang, Hualin Yang, Weihua Gao, Mingyuan Liu, Xiaolei Liu, Yu Zhou
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270
Acceso en línea:	https://doaj.org/article/db94952b166648b4b3c6244071d92871
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spelling	oai:doaj.org-article:db94952b166648b4b3c6244071d928712021-12-02T20:24:16ZRecombinant cystatin-like protein-based competition ELISA for Trichinella spiralis antibody test in multihost sera.1935-27271935-273510.1371/journal.pntd.0009723https://doaj.org/article/db94952b166648b4b3c6244071d928712021-08-01T00:00:00Zhttps://doi.org/10.1371/journal.pntd.0009723https://doaj.org/toc/1935-2727https://doaj.org/toc/1935-2735<h4>Objectives</h4>Trichinella spiralis is a zoonotic parasite with a complex parasitic life cycle and exposed to animals or humans by infectious meat. To control transmissions of T. spiralis through the food chain to humans, sensitive and selective multihost sera-diagnosis is urgent needed for monitoring T. spiralis exposure.<h4>Methods</h4>A competition enzyme-linked immunosorbent assay (cELISA) for T. spiralis infection diagnosis in multihost sera was developed based on recombinant cystatin-like protein (rCLP-cELISA) as well as monoclonal antibodies. The sensitivity and accuracy of the rCLP-cELISA were quantified using swine (n = 1316), mice (n = 189) and human (n = 157) serum samples. T. spiralis-antibody targeting test ability of the rCLP-cELISA in swine (n = 22) and human (n = 36), instead of other parasites or viruses antibodies, was evaluated.<h4>Results</h4>The rCLP-cELISA showed high agreement with commercial ELISA kits in field swine sera assessed by Cohen's kappa value (κ = 0.7963). And it showed 100% specificity in human trichinellosis detection with sensitivity of 96.49%, no cross-reaction with other parasite or virus infections, and high positive detection rate of 87.5% in low-dose infected swine. Besides, the rCLP-cELISA exhibited potential in the detection of T. spiralis, T. nelsoni and Trichinella T8 infections.<h4>Conclusions</h4>The rCLP-cELISA can be used for T. spiralis-associated antibody test in multihost sera.Yan LiuNing XuYansong LiBin TangHualin YangWeihua GaoMingyuan LiuXiaolei LiuYu ZhouPublic Library of Science (PLoS)articleArctic medicine. Tropical medicineRC955-962Public aspects of medicineRA1-1270ENPLoS Neglected Tropical Diseases, Vol 15, Iss 8, p e0009723 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270
spellingShingle	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Yan Liu Ning Xu Yansong Li Bin Tang Hualin Yang Weihua Gao Mingyuan Liu Xiaolei Liu Yu Zhou Recombinant cystatin-like protein-based competition ELISA for Trichinella spiralis antibody test in multihost sera.
description	<h4>Objectives</h4>Trichinella spiralis is a zoonotic parasite with a complex parasitic life cycle and exposed to animals or humans by infectious meat. To control transmissions of T. spiralis through the food chain to humans, sensitive and selective multihost sera-diagnosis is urgent needed for monitoring T. spiralis exposure.<h4>Methods</h4>A competition enzyme-linked immunosorbent assay (cELISA) for T. spiralis infection diagnosis in multihost sera was developed based on recombinant cystatin-like protein (rCLP-cELISA) as well as monoclonal antibodies. The sensitivity and accuracy of the rCLP-cELISA were quantified using swine (n = 1316), mice (n = 189) and human (n = 157) serum samples. T. spiralis-antibody targeting test ability of the rCLP-cELISA in swine (n = 22) and human (n = 36), instead of other parasites or viruses antibodies, was evaluated.<h4>Results</h4>The rCLP-cELISA showed high agreement with commercial ELISA kits in field swine sera assessed by Cohen's kappa value (κ = 0.7963). And it showed 100% specificity in human trichinellosis detection with sensitivity of 96.49%, no cross-reaction with other parasite or virus infections, and high positive detection rate of 87.5% in low-dose infected swine. Besides, the rCLP-cELISA exhibited potential in the detection of T. spiralis, T. nelsoni and Trichinella T8 infections.<h4>Conclusions</h4>The rCLP-cELISA can be used for T. spiralis-associated antibody test in multihost sera.
format	article
author	Yan Liu Ning Xu Yansong Li Bin Tang Hualin Yang Weihua Gao Mingyuan Liu Xiaolei Liu Yu Zhou
author_facet	Yan Liu Ning Xu Yansong Li Bin Tang Hualin Yang Weihua Gao Mingyuan Liu Xiaolei Liu Yu Zhou
author_sort	Yan Liu
title	Recombinant cystatin-like protein-based competition ELISA for Trichinella spiralis antibody test in multihost sera.
title_short	Recombinant cystatin-like protein-based competition ELISA for Trichinella spiralis antibody test in multihost sera.
title_full	Recombinant cystatin-like protein-based competition ELISA for Trichinella spiralis antibody test in multihost sera.
title_fullStr	Recombinant cystatin-like protein-based competition ELISA for Trichinella spiralis antibody test in multihost sera.
title_full_unstemmed	Recombinant cystatin-like protein-based competition ELISA for Trichinella spiralis antibody test in multihost sera.
title_sort	recombinant cystatin-like protein-based competition elisa for trichinella spiralis antibody test in multihost sera.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/db94952b166648b4b3c6244071d92871
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Recombinant cystatin-like protein-based competition ELISA for Trichinella spiralis antibody test in multihost sera.

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