Genome-wide DNA methylation and gene expression analyses of monozygotic twins discordant for intelligence levels.

Human intelligence, as measured by intelligence quotient (IQ) tests, demonstrates one of the highest heritabilities among human quantitative traits. Nevertheless, studies to identify quantitative trait loci responsible for intelligence face challenges because of the small effect sizes of individual...

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Autores principales:	Chih-Chieh Yu, Mari Furukawa, Kazuhiro Kobayashi, Chizuru Shikishima, Pei-Chieng Cha, Jun Sese, Hiroko Sugawara, Kazuya Iwamoto, Tadafumi Kato, Juko Ando, Tatsushi Toda
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/db94cf8f23714085899ebbcb8ab8235f
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spelling	oai:doaj.org-article:db94cf8f23714085899ebbcb8ab8235f2021-11-18T08:11:43ZGenome-wide DNA methylation and gene expression analyses of monozygotic twins discordant for intelligence levels.1932-620310.1371/journal.pone.0047081https://doaj.org/article/db94cf8f23714085899ebbcb8ab8235f2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23082141/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Human intelligence, as measured by intelligence quotient (IQ) tests, demonstrates one of the highest heritabilities among human quantitative traits. Nevertheless, studies to identify quantitative trait loci responsible for intelligence face challenges because of the small effect sizes of individual genes. Phenotypically discordant monozygotic (MZ) twins provide a feasible way to minimize the effects of irrelevant genetic and environmental factors, and should yield more interpretable results by finding epigenetic or gene expression differences between twins. Here we conducted array-based genome-wide DNA methylation and gene expression analyses using 17 pairs of healthy MZ twins discordant intelligently. ARHGAP18, related to Rho GTPase, was identified in pair-wise methylation status analysis and validated via direct bisulfite sequencing and quantitative RT-PCR. To perform expression profile analysis, gene set enrichment analysis (GSEA) between the groups of twins with higher IQ and their co-twins revealed up-regulated expression of several ribosome-related genes and DNA replication-related genes in the group with higher IQ. To focus more on individual pairs, we conducted pair-wise GSEA and leading edge analysis, which indicated up-regulated expression of several ion channel-related genes in twins with lower IQ. Our findings implied that these groups of genes may be related to IQ and should shed light on the mechanism underlying human intelligence.Chih-Chieh YuMari FurukawaKazuhiro KobayashiChizuru ShikishimaPei-Chieng ChaJun SeseHiroko SugawaraKazuya IwamotoTadafumi KatoJuko AndoTatsushi TodaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 10, p e47081 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chih-Chieh Yu Mari Furukawa Kazuhiro Kobayashi Chizuru Shikishima Pei-Chieng Cha Jun Sese Hiroko Sugawara Kazuya Iwamoto Tadafumi Kato Juko Ando Tatsushi Toda Genome-wide DNA methylation and gene expression analyses of monozygotic twins discordant for intelligence levels.
description	Human intelligence, as measured by intelligence quotient (IQ) tests, demonstrates one of the highest heritabilities among human quantitative traits. Nevertheless, studies to identify quantitative trait loci responsible for intelligence face challenges because of the small effect sizes of individual genes. Phenotypically discordant monozygotic (MZ) twins provide a feasible way to minimize the effects of irrelevant genetic and environmental factors, and should yield more interpretable results by finding epigenetic or gene expression differences between twins. Here we conducted array-based genome-wide DNA methylation and gene expression analyses using 17 pairs of healthy MZ twins discordant intelligently. ARHGAP18, related to Rho GTPase, was identified in pair-wise methylation status analysis and validated via direct bisulfite sequencing and quantitative RT-PCR. To perform expression profile analysis, gene set enrichment analysis (GSEA) between the groups of twins with higher IQ and their co-twins revealed up-regulated expression of several ribosome-related genes and DNA replication-related genes in the group with higher IQ. To focus more on individual pairs, we conducted pair-wise GSEA and leading edge analysis, which indicated up-regulated expression of several ion channel-related genes in twins with lower IQ. Our findings implied that these groups of genes may be related to IQ and should shed light on the mechanism underlying human intelligence.
format	article
author	Chih-Chieh Yu Mari Furukawa Kazuhiro Kobayashi Chizuru Shikishima Pei-Chieng Cha Jun Sese Hiroko Sugawara Kazuya Iwamoto Tadafumi Kato Juko Ando Tatsushi Toda
author_facet	Chih-Chieh Yu Mari Furukawa Kazuhiro Kobayashi Chizuru Shikishima Pei-Chieng Cha Jun Sese Hiroko Sugawara Kazuya Iwamoto Tadafumi Kato Juko Ando Tatsushi Toda
author_sort	Chih-Chieh Yu
title	Genome-wide DNA methylation and gene expression analyses of monozygotic twins discordant for intelligence levels.
title_short	Genome-wide DNA methylation and gene expression analyses of monozygotic twins discordant for intelligence levels.
title_full	Genome-wide DNA methylation and gene expression analyses of monozygotic twins discordant for intelligence levels.
title_fullStr	Genome-wide DNA methylation and gene expression analyses of monozygotic twins discordant for intelligence levels.
title_full_unstemmed	Genome-wide DNA methylation and gene expression analyses of monozygotic twins discordant for intelligence levels.
title_sort	genome-wide dna methylation and gene expression analyses of monozygotic twins discordant for intelligence levels.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/db94cf8f23714085899ebbcb8ab8235f
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Genome-wide DNA methylation and gene expression analyses of monozygotic twins discordant for intelligence levels.

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