Scalable continuous evolution for the generation of diverse enzyme variants encompassing promiscuous activities

Generating rich ortholog diversity for biocatalysts can be difficult due to the deep evolutionary processes involved. Here the authors use OrthoRep to rapidly evolve TrpB to produce sequence-diverse variants with altered substrate promiscuity.

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Autores principales:	Gordon Rix, Ella J. Watkins-Dulaney, Patrick J. Almhjell, Christina E. Boville, Frances H. Arnold, Chang C. Liu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/db9749500e5f43c9938165f0f7bdc6be
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spelling	oai:doaj.org-article:db9749500e5f43c9938165f0f7bdc6be2021-12-02T17:32:13ZScalable continuous evolution for the generation of diverse enzyme variants encompassing promiscuous activities10.1038/s41467-020-19539-62041-1723https://doaj.org/article/db9749500e5f43c9938165f0f7bdc6be2020-11-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-19539-6https://doaj.org/toc/2041-1723Generating rich ortholog diversity for biocatalysts can be difficult due to the deep evolutionary processes involved. Here the authors use OrthoRep to rapidly evolve TrpB to produce sequence-diverse variants with altered substrate promiscuity.Gordon RixElla J. Watkins-DulaneyPatrick J. AlmhjellChristina E. BovilleFrances H. ArnoldChang C. LiuNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-11 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Gordon Rix Ella J. Watkins-Dulaney Patrick J. Almhjell Christina E. Boville Frances H. Arnold Chang C. Liu Scalable continuous evolution for the generation of diverse enzyme variants encompassing promiscuous activities
description	Generating rich ortholog diversity for biocatalysts can be difficult due to the deep evolutionary processes involved. Here the authors use OrthoRep to rapidly evolve TrpB to produce sequence-diverse variants with altered substrate promiscuity.
format	article
author	Gordon Rix Ella J. Watkins-Dulaney Patrick J. Almhjell Christina E. Boville Frances H. Arnold Chang C. Liu
author_facet	Gordon Rix Ella J. Watkins-Dulaney Patrick J. Almhjell Christina E. Boville Frances H. Arnold Chang C. Liu
author_sort	Gordon Rix
title	Scalable continuous evolution for the generation of diverse enzyme variants encompassing promiscuous activities
title_short	Scalable continuous evolution for the generation of diverse enzyme variants encompassing promiscuous activities
title_full	Scalable continuous evolution for the generation of diverse enzyme variants encompassing promiscuous activities
title_fullStr	Scalable continuous evolution for the generation of diverse enzyme variants encompassing promiscuous activities
title_full_unstemmed	Scalable continuous evolution for the generation of diverse enzyme variants encompassing promiscuous activities
title_sort	scalable continuous evolution for the generation of diverse enzyme variants encompassing promiscuous activities
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/db9749500e5f43c9938165f0f7bdc6be
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Scalable continuous evolution for the generation of diverse enzyme variants encompassing promiscuous activities

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