Properties of peptides released from salmon and carp via simulated human-like gastrointestinal digestion described applying quantitative parameters.

Apart from the classical (experimental) methods, biologically active peptides can be studied via bioinformatics approach, also known as in silico analysis. This study aimed to verify the following research hypothesis: ACE inhibitors and antioxidant peptides can be released from salmon and carp prote...

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Autores principales:	Justyna Borawska-Dziadkiewicz, Małgorzata Darewicz, Anna Sylwia Tarczyńska
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/db98de77d4b14138987acccf8527abda
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spelling	oai:doaj.org-article:db98de77d4b14138987acccf8527abda2021-12-02T20:18:26ZProperties of peptides released from salmon and carp via simulated human-like gastrointestinal digestion described applying quantitative parameters.1932-620310.1371/journal.pone.0255969https://doaj.org/article/db98de77d4b14138987acccf8527abda2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0255969https://doaj.org/toc/1932-6203Apart from the classical (experimental) methods, biologically active peptides can be studied via bioinformatics approach, also known as in silico analysis. This study aimed to verify the following research hypothesis: ACE inhibitors and antioxidant peptides can be released from salmon and carp proteins during simulated in silico human-like gastrointestinal digestion. The potential to release biopeptides was evaluated using the BIOPEP-UWM quantitative criteria including the profile of biological activity, frequency of the occurrence (A)/release (AE) of fragments with an ACE inhibitory or antioxidant activity by selected enzymes, and relative frequency of release of bioactive fragments with a given activity by selected enzymes (W). Salmon collagen and myofibrillar proteins of carp turned out to be the best potential source of the searched peptides-ACE inhibitors and antioxidant peptides. Nonetheless, after digestion, the highest numbers of ACE inhibitors and antioxidant peptides were potentially released from the myofibrillar proteins of salmon and carp. Peptide Ranker Score, Pepsite2, and ADMETlab platform were applied to evaluate peptides' bioactivity potential, their safety and drug-like properties. Among the 63 sequences obtained after the simulated digestion of salmon and carp proteins, 30 were considered potential biopeptides. The amino acid sequences of ACE-inhibiting and antioxidant peptides were predominated by P, G, F, W, R, and L. The predicted high probability of absorption of most analyzed peptides and their low toxicity should be considered as their advantage.Justyna Borawska-DziadkiewiczMałgorzata DarewiczAnna Sylwia TarczyńskaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 8, p e0255969 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Justyna Borawska-Dziadkiewicz Małgorzata Darewicz Anna Sylwia Tarczyńska Properties of peptides released from salmon and carp via simulated human-like gastrointestinal digestion described applying quantitative parameters.
description	Apart from the classical (experimental) methods, biologically active peptides can be studied via bioinformatics approach, also known as in silico analysis. This study aimed to verify the following research hypothesis: ACE inhibitors and antioxidant peptides can be released from salmon and carp proteins during simulated in silico human-like gastrointestinal digestion. The potential to release biopeptides was evaluated using the BIOPEP-UWM quantitative criteria including the profile of biological activity, frequency of the occurrence (A)/release (AE) of fragments with an ACE inhibitory or antioxidant activity by selected enzymes, and relative frequency of release of bioactive fragments with a given activity by selected enzymes (W). Salmon collagen and myofibrillar proteins of carp turned out to be the best potential source of the searched peptides-ACE inhibitors and antioxidant peptides. Nonetheless, after digestion, the highest numbers of ACE inhibitors and antioxidant peptides were potentially released from the myofibrillar proteins of salmon and carp. Peptide Ranker Score, Pepsite2, and ADMETlab platform were applied to evaluate peptides' bioactivity potential, their safety and drug-like properties. Among the 63 sequences obtained after the simulated digestion of salmon and carp proteins, 30 were considered potential biopeptides. The amino acid sequences of ACE-inhibiting and antioxidant peptides were predominated by P, G, F, W, R, and L. The predicted high probability of absorption of most analyzed peptides and their low toxicity should be considered as their advantage.
format	article
author	Justyna Borawska-Dziadkiewicz Małgorzata Darewicz Anna Sylwia Tarczyńska
author_facet	Justyna Borawska-Dziadkiewicz Małgorzata Darewicz Anna Sylwia Tarczyńska
author_sort	Justyna Borawska-Dziadkiewicz
title	Properties of peptides released from salmon and carp via simulated human-like gastrointestinal digestion described applying quantitative parameters.
title_short	Properties of peptides released from salmon and carp via simulated human-like gastrointestinal digestion described applying quantitative parameters.
title_full	Properties of peptides released from salmon and carp via simulated human-like gastrointestinal digestion described applying quantitative parameters.
title_fullStr	Properties of peptides released from salmon and carp via simulated human-like gastrointestinal digestion described applying quantitative parameters.
title_full_unstemmed	Properties of peptides released from salmon and carp via simulated human-like gastrointestinal digestion described applying quantitative parameters.
title_sort	properties of peptides released from salmon and carp via simulated human-like gastrointestinal digestion described applying quantitative parameters.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/db98de77d4b14138987acccf8527abda
work_keys_str_mv	AT justynaborawskadziadkiewicz propertiesofpeptidesreleasedfromsalmonandcarpviasimulatedhumanlikegastrointestinaldigestiondescribedapplyingquantitativeparameters AT małgorzatadarewicz propertiesofpeptidesreleasedfromsalmonandcarpviasimulatedhumanlikegastrointestinaldigestiondescribedapplyingquantitativeparameters AT annasylwiatarczynska propertiesofpeptidesreleasedfromsalmonandcarpviasimulatedhumanlikegastrointestinaldigestiondescribedapplyingquantitativeparameters
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Properties of peptides released from salmon and carp via simulated human-like gastrointestinal digestion described applying quantitative parameters.

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