Harnessing efficient multiplex PCR methods to detect the expanding Tet(X) family of tigecycline resistance genes

A growing number of tet(X)-type tigecycline resistance determinants [tet(X1) to tet(X5)] constitutes an expanding family of tetracycline-inactivating enzymes, posing a potential risk to global public health. Here, we report the development of an efficient multiplex PCR method to detect the family of...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Kai Ji, Yongchang Xu, Jian Sun, Man Huang, Xu Jia, Chengjian Jiang, Youjun Feng
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2020
Materias:	multiplex pcr antimicrobial resistance tigecycline resistance tet(x) tet(x1) tet(x2) tet(x3) tet(x4) tet(x5) Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/db9e4eb765a54fa6bb02b807dff7a747
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:db9e4eb765a54fa6bb02b807dff7a747
record_format	dspace
spelling	oai:doaj.org-article:db9e4eb765a54fa6bb02b807dff7a7472021-11-17T14:21:57ZHarnessing efficient multiplex PCR methods to detect the expanding Tet(X) family of tigecycline resistance genes2150-55942150-560810.1080/21505594.2019.1706913https://doaj.org/article/db9e4eb765a54fa6bb02b807dff7a7472020-12-01T00:00:00Zhttp://dx.doi.org/10.1080/21505594.2019.1706913https://doaj.org/toc/2150-5594https://doaj.org/toc/2150-5608A growing number of tet(X)-type tigecycline resistance determinants [tet(X1) to tet(X5)] constitutes an expanding family of tetracycline-inactivating enzymes, posing a potential risk to global public health. Here, we report the development of an efficient multiplex PCR method to detect the family of tet(X) variants. This method is successfully applied in the screen and validation of tet(X) genes in the field and clinic bacterial samples. In addition, we found that the formerly proposed tet(X1) is a premature truncated version by the inappropriate annotation, and fixed this error. Overall, it might be the first genetic tool for the detection of different Tet(X) members.Kai JiYongchang XuJian SunMan HuangXu JiaChengjian JiangYoujun FengTaylor & Francis Grouparticlemultiplex pcrantimicrobial resistancetigecycline resistancetet(x)tet(x1)tet(x2)tet(x3)tet(x4)tet(x5)Infectious and parasitic diseasesRC109-216ENVirulence, Vol 11, Iss 1, Pp 49-56 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	multiplex pcr antimicrobial resistance tigecycline resistance tet(x) tet(x1) tet(x2) tet(x3) tet(x4) tet(x5) Infectious and parasitic diseases RC109-216
spellingShingle	multiplex pcr antimicrobial resistance tigecycline resistance tet(x) tet(x1) tet(x2) tet(x3) tet(x4) tet(x5) Infectious and parasitic diseases RC109-216 Kai Ji Yongchang Xu Jian Sun Man Huang Xu Jia Chengjian Jiang Youjun Feng Harnessing efficient multiplex PCR methods to detect the expanding Tet(X) family of tigecycline resistance genes
description	A growing number of tet(X)-type tigecycline resistance determinants [tet(X1) to tet(X5)] constitutes an expanding family of tetracycline-inactivating enzymes, posing a potential risk to global public health. Here, we report the development of an efficient multiplex PCR method to detect the family of tet(X) variants. This method is successfully applied in the screen and validation of tet(X) genes in the field and clinic bacterial samples. In addition, we found that the formerly proposed tet(X1) is a premature truncated version by the inappropriate annotation, and fixed this error. Overall, it might be the first genetic tool for the detection of different Tet(X) members.
format	article
author	Kai Ji Yongchang Xu Jian Sun Man Huang Xu Jia Chengjian Jiang Youjun Feng
author_facet	Kai Ji Yongchang Xu Jian Sun Man Huang Xu Jia Chengjian Jiang Youjun Feng
author_sort	Kai Ji
title	Harnessing efficient multiplex PCR methods to detect the expanding Tet(X) family of tigecycline resistance genes
title_short	Harnessing efficient multiplex PCR methods to detect the expanding Tet(X) family of tigecycline resistance genes
title_full	Harnessing efficient multiplex PCR methods to detect the expanding Tet(X) family of tigecycline resistance genes
title_fullStr	Harnessing efficient multiplex PCR methods to detect the expanding Tet(X) family of tigecycline resistance genes
title_full_unstemmed	Harnessing efficient multiplex PCR methods to detect the expanding Tet(X) family of tigecycline resistance genes
title_sort	harnessing efficient multiplex pcr methods to detect the expanding tet(x) family of tigecycline resistance genes
publisher	Taylor & Francis Group
publishDate	2020
url	https://doaj.org/article/db9e4eb765a54fa6bb02b807dff7a747
work_keys_str_mv	AT kaiji harnessingefficientmultiplexpcrmethodstodetecttheexpandingtetxfamilyoftigecyclineresistancegenes AT yongchangxu harnessingefficientmultiplexpcrmethodstodetecttheexpandingtetxfamilyoftigecyclineresistancegenes AT jiansun harnessingefficientmultiplexpcrmethodstodetecttheexpandingtetxfamilyoftigecyclineresistancegenes AT manhuang harnessingefficientmultiplexpcrmethodstodetecttheexpandingtetxfamilyoftigecyclineresistancegenes AT xujia harnessingefficientmultiplexpcrmethodstodetecttheexpandingtetxfamilyoftigecyclineresistancegenes AT chengjianjiang harnessingefficientmultiplexpcrmethodstodetecttheexpandingtetxfamilyoftigecyclineresistancegenes AT youjunfeng harnessingefficientmultiplexpcrmethodstodetecttheexpandingtetxfamilyoftigecyclineresistancegenes
_version_	1718425514912251904

Harnessing efficient multiplex PCR methods to detect the expanding Tet(X) family of tigecycline resistance genes

Ejemplares similares