Hearing impairment and associated morphological changes in pituitary adenylate cyclase activating polypeptide (PACAP)-deficient mice

Abstract Pituitary adenylate cyclase activating polypeptide (PACAP) is a regulatory and cytoprotective neuropeptide, its deficiency implies accelerated aging in mice. It is present in the auditory system having antiapoptotic effects. Expression of Ca2+-binding proteins and its PAC1 receptor differs...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Daniel Balazs Fulop, Viktoria Humli, Judit Szepesy, Virag Ott, Dora Reglodi, Balazs Gaszner, Adrienn Nemeth, Agnes Szirmai, Laszlo Tamas, Hitoshi Hashimoto, Tibor Zelles, Andrea Tamas
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/dba40cbdf54047d6b67a8e21022ef6df
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:dba40cbdf54047d6b67a8e21022ef6df
record_format	dspace
spelling	oai:doaj.org-article:dba40cbdf54047d6b67a8e21022ef6df2021-12-02T15:09:42ZHearing impairment and associated morphological changes in pituitary adenylate cyclase activating polypeptide (PACAP)-deficient mice10.1038/s41598-019-50775-z2045-2322https://doaj.org/article/dba40cbdf54047d6b67a8e21022ef6df2019-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-50775-zhttps://doaj.org/toc/2045-2322Abstract Pituitary adenylate cyclase activating polypeptide (PACAP) is a regulatory and cytoprotective neuropeptide, its deficiency implies accelerated aging in mice. It is present in the auditory system having antiapoptotic effects. Expression of Ca2+-binding proteins and its PAC1 receptor differs in the inner ear of PACAP-deficient (KO) and wild-type (WT) mice. Our aim was to elucidate the functional role of PACAP in the auditory system. Auditory brainstem response (ABR) tests found higher hearing thresholds in KO mice at click and low frequency burst stimuli. Hearing impairment at higher frequencies showed as reduced ABR wave amplitudes and latencies in KO animals. Increase in neuronal activity, demonstrated by c-Fos immunolabeling, was lower in KO mice after noise exposure in the ventral and dorsal cochlear nuclei. Noise induced neuronal activation was similar in further relay nuclei of the auditory pathway of WT and KO mice. Based on the similar inflammatory and angiogenic protein profile data from cochlear duct lysates, neither inflammation nor disturbed angiogenesis, as potential pathological components in sensorineural hearing losses, seem to be involved in the pathomechanism of the presented functional and morphological changes in PACAP KO mice. The hearing impairment is probably concomitant with the markedly accelerated aging processes in these animals.Daniel Balazs FulopViktoria HumliJudit SzepesyVirag OttDora ReglodiBalazs GasznerAdrienn NemethAgnes SzirmaiLaszlo TamasHitoshi HashimotoTibor ZellesAndrea TamasNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-15 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Daniel Balazs Fulop Viktoria Humli Judit Szepesy Virag Ott Dora Reglodi Balazs Gaszner Adrienn Nemeth Agnes Szirmai Laszlo Tamas Hitoshi Hashimoto Tibor Zelles Andrea Tamas Hearing impairment and associated morphological changes in pituitary adenylate cyclase activating polypeptide (PACAP)-deficient mice
description	Abstract Pituitary adenylate cyclase activating polypeptide (PACAP) is a regulatory and cytoprotective neuropeptide, its deficiency implies accelerated aging in mice. It is present in the auditory system having antiapoptotic effects. Expression of Ca2+-binding proteins and its PAC1 receptor differs in the inner ear of PACAP-deficient (KO) and wild-type (WT) mice. Our aim was to elucidate the functional role of PACAP in the auditory system. Auditory brainstem response (ABR) tests found higher hearing thresholds in KO mice at click and low frequency burst stimuli. Hearing impairment at higher frequencies showed as reduced ABR wave amplitudes and latencies in KO animals. Increase in neuronal activity, demonstrated by c-Fos immunolabeling, was lower in KO mice after noise exposure in the ventral and dorsal cochlear nuclei. Noise induced neuronal activation was similar in further relay nuclei of the auditory pathway of WT and KO mice. Based on the similar inflammatory and angiogenic protein profile data from cochlear duct lysates, neither inflammation nor disturbed angiogenesis, as potential pathological components in sensorineural hearing losses, seem to be involved in the pathomechanism of the presented functional and morphological changes in PACAP KO mice. The hearing impairment is probably concomitant with the markedly accelerated aging processes in these animals.
format	article
author	Daniel Balazs Fulop Viktoria Humli Judit Szepesy Virag Ott Dora Reglodi Balazs Gaszner Adrienn Nemeth Agnes Szirmai Laszlo Tamas Hitoshi Hashimoto Tibor Zelles Andrea Tamas
author_facet	Daniel Balazs Fulop Viktoria Humli Judit Szepesy Virag Ott Dora Reglodi Balazs Gaszner Adrienn Nemeth Agnes Szirmai Laszlo Tamas Hitoshi Hashimoto Tibor Zelles Andrea Tamas
author_sort	Daniel Balazs Fulop
title	Hearing impairment and associated morphological changes in pituitary adenylate cyclase activating polypeptide (PACAP)-deficient mice
title_short	Hearing impairment and associated morphological changes in pituitary adenylate cyclase activating polypeptide (PACAP)-deficient mice
title_full	Hearing impairment and associated morphological changes in pituitary adenylate cyclase activating polypeptide (PACAP)-deficient mice
title_fullStr	Hearing impairment and associated morphological changes in pituitary adenylate cyclase activating polypeptide (PACAP)-deficient mice
title_full_unstemmed	Hearing impairment and associated morphological changes in pituitary adenylate cyclase activating polypeptide (PACAP)-deficient mice
title_sort	hearing impairment and associated morphological changes in pituitary adenylate cyclase activating polypeptide (pacap)-deficient mice
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/dba40cbdf54047d6b67a8e21022ef6df
work_keys_str_mv	AT danielbalazsfulop hearingimpairmentandassociatedmorphologicalchangesinpituitaryadenylatecyclaseactivatingpolypeptidepacapdeficientmice AT viktoriahumli hearingimpairmentandassociatedmorphologicalchangesinpituitaryadenylatecyclaseactivatingpolypeptidepacapdeficientmice AT juditszepesy hearingimpairmentandassociatedmorphologicalchangesinpituitaryadenylatecyclaseactivatingpolypeptidepacapdeficientmice AT viragott hearingimpairmentandassociatedmorphologicalchangesinpituitaryadenylatecyclaseactivatingpolypeptidepacapdeficientmice AT dorareglodi hearingimpairmentandassociatedmorphologicalchangesinpituitaryadenylatecyclaseactivatingpolypeptidepacapdeficientmice AT balazsgaszner hearingimpairmentandassociatedmorphologicalchangesinpituitaryadenylatecyclaseactivatingpolypeptidepacapdeficientmice AT adriennnemeth hearingimpairmentandassociatedmorphologicalchangesinpituitaryadenylatecyclaseactivatingpolypeptidepacapdeficientmice AT agnesszirmai hearingimpairmentandassociatedmorphologicalchangesinpituitaryadenylatecyclaseactivatingpolypeptidepacapdeficientmice AT laszlotamas hearingimpairmentandassociatedmorphologicalchangesinpituitaryadenylatecyclaseactivatingpolypeptidepacapdeficientmice AT hitoshihashimoto hearingimpairmentandassociatedmorphologicalchangesinpituitaryadenylatecyclaseactivatingpolypeptidepacapdeficientmice AT tiborzelles hearingimpairmentandassociatedmorphologicalchangesinpituitaryadenylatecyclaseactivatingpolypeptidepacapdeficientmice AT andreatamas hearingimpairmentandassociatedmorphologicalchangesinpituitaryadenylatecyclaseactivatingpolypeptidepacapdeficientmice
_version_	1718387783481950208

Hearing impairment and associated morphological changes in pituitary adenylate cyclase activating polypeptide (PACAP)-deficient mice

Ejemplares similares