<i>Ascaris suum</i> Nutrient Uptake and Metabolic Release, and Modulation of Host Intestinal Nutrient Transport by Excretory-Secretory and Cuticle Antigens In Vitro

<i>Ascaris suum</i>, the most important pig parasite, also infects humans as a zoonotic pathogen. Malabsorption upon infection probably results from impaired nutrient transport, presumably mediated by the parasite’s excretory-secretory (ES) or cuticle somatic (CSO) antigens. The present...

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Autores principales:	Sarina Koehler, Andrea Springer, Nicole Issel, Stefanie Klinger, Michael Wendt, Gerhard Breves, Christina Strube
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	Ascariosis Ascariasis soil-transmitted helminths glucose transport peptide transport amino acid transport Medicine R
Acceso en línea:	https://doaj.org/article/dba8cdd86a8749fca8f81d1fe2b9f930
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spelling	oai:doaj.org-article:dba8cdd86a8749fca8f81d1fe2b9f9302021-11-25T18:38:07Z<i>Ascaris suum</i> Nutrient Uptake and Metabolic Release, and Modulation of Host Intestinal Nutrient Transport by Excretory-Secretory and Cuticle Antigens In Vitro10.3390/pathogens101114192076-0817https://doaj.org/article/dba8cdd86a8749fca8f81d1fe2b9f9302021-11-01T00:00:00Zhttps://www.mdpi.com/2076-0817/10/11/1419https://doaj.org/toc/2076-0817<i>Ascaris suum</i>, the most important pig parasite, also infects humans as a zoonotic pathogen. Malabsorption upon infection probably results from impaired nutrient transport, presumably mediated by the parasite’s excretory-secretory (ES) or cuticle somatic (CSO) antigens. The present study investigated the electrogenic transport (ΔI<sub>sc</sub>) of glucose, alanine and the dipeptide glycyl-l-glutamine (glygln), as well as glucose net flux rates in pig jejunal tissue after in vitro exposure to adult <i>A. suum</i> total ES or CSO antigens in Ussing chambers. ΔI<sub>sc</sub> of glucose, alanine and glucose net flux rate were significantly decreased after one hour of exposure to total ES antigen. In contrast, CSO antigens increased the transport of glygln. Additionally, nutrient uptake and ES antigen pattern were compared in culture medium from untreated adult worms and those with sealed mouth and anal openings. Untreated worms completely absorbed glucose, while cuticular absorption in sealed worms led to 90% reduction. Amino acid absorption was 30% less effective in sealed worms, and ammonia excretion decreased by 20%. Overall, the results show that <i>A. suum</i> total ES antigen rapidly impairs nutrient transport in vitro. Future studies confirming the results in vivo, narrowing down the ES components responsible and investigating underlying molecular mechanisms are needed.Sarina KoehlerAndrea SpringerNicole IsselStefanie KlingerMichael WendtGerhard BrevesChristina StrubeMDPI AGarticleAscariosisAscariasissoil-transmitted helminthsglucose transportpeptide transportamino acid transportMedicineRENPathogens, Vol 10, Iss 1419, p 1419 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Ascariosis Ascariasis soil-transmitted helminths glucose transport peptide transport amino acid transport Medicine R
spellingShingle	Ascariosis Ascariasis soil-transmitted helminths glucose transport peptide transport amino acid transport Medicine R Sarina Koehler Andrea Springer Nicole Issel Stefanie Klinger Michael Wendt Gerhard Breves Christina Strube <i>Ascaris suum</i> Nutrient Uptake and Metabolic Release, and Modulation of Host Intestinal Nutrient Transport by Excretory-Secretory and Cuticle Antigens In Vitro
description	<i>Ascaris suum</i>, the most important pig parasite, also infects humans as a zoonotic pathogen. Malabsorption upon infection probably results from impaired nutrient transport, presumably mediated by the parasite’s excretory-secretory (ES) or cuticle somatic (CSO) antigens. The present study investigated the electrogenic transport (ΔI<sub>sc</sub>) of glucose, alanine and the dipeptide glycyl-l-glutamine (glygln), as well as glucose net flux rates in pig jejunal tissue after in vitro exposure to adult <i>A. suum</i> total ES or CSO antigens in Ussing chambers. ΔI<sub>sc</sub> of glucose, alanine and glucose net flux rate were significantly decreased after one hour of exposure to total ES antigen. In contrast, CSO antigens increased the transport of glygln. Additionally, nutrient uptake and ES antigen pattern were compared in culture medium from untreated adult worms and those with sealed mouth and anal openings. Untreated worms completely absorbed glucose, while cuticular absorption in sealed worms led to 90% reduction. Amino acid absorption was 30% less effective in sealed worms, and ammonia excretion decreased by 20%. Overall, the results show that <i>A. suum</i> total ES antigen rapidly impairs nutrient transport in vitro. Future studies confirming the results in vivo, narrowing down the ES components responsible and investigating underlying molecular mechanisms are needed.
format	article
author	Sarina Koehler Andrea Springer Nicole Issel Stefanie Klinger Michael Wendt Gerhard Breves Christina Strube
author_facet	Sarina Koehler Andrea Springer Nicole Issel Stefanie Klinger Michael Wendt Gerhard Breves Christina Strube
author_sort	Sarina Koehler
title	<i>Ascaris suum</i> Nutrient Uptake and Metabolic Release, and Modulation of Host Intestinal Nutrient Transport by Excretory-Secretory and Cuticle Antigens In Vitro
title_short	<i>Ascaris suum</i> Nutrient Uptake and Metabolic Release, and Modulation of Host Intestinal Nutrient Transport by Excretory-Secretory and Cuticle Antigens In Vitro
title_full	<i>Ascaris suum</i> Nutrient Uptake and Metabolic Release, and Modulation of Host Intestinal Nutrient Transport by Excretory-Secretory and Cuticle Antigens In Vitro
title_fullStr	<i>Ascaris suum</i> Nutrient Uptake and Metabolic Release, and Modulation of Host Intestinal Nutrient Transport by Excretory-Secretory and Cuticle Antigens In Vitro
title_full_unstemmed	<i>Ascaris suum</i> Nutrient Uptake and Metabolic Release, and Modulation of Host Intestinal Nutrient Transport by Excretory-Secretory and Cuticle Antigens In Vitro
title_sort	<i>ascaris suum</i> nutrient uptake and metabolic release, and modulation of host intestinal nutrient transport by excretory-secretory and cuticle antigens in vitro
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/dba8cdd86a8749fca8f81d1fe2b9f930
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<i>Ascaris suum</i> Nutrient Uptake and Metabolic Release, and Modulation of Host Intestinal Nutrient Transport by Excretory-Secretory and Cuticle Antigens In Vitro

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