Enhanced production and biochemical characterization of a thermostable amylase from thermophilic bacterium Geobacillus icigianus BITSNS038

The current study focuses on optimizing, characterizing, and purifying a thermostable amylase from thermophilic bacteria Geobacillus icigianus (BITSNS038). The amylase was produced optimally at 18–24 h of incubation in the presence of starch and tryptone at 7.5 and 3.0 g/L, respectively (pH 7.0, 70°...

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Autores principales:	Snehi Soy, Vinod Kumar Nigam, Shubha Rani Sharma
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	geobacillus kinetic modeling saccharification thermostable amylase ultrafiltration Science (General) Q1-390
Acceso en línea:	https://doaj.org/article/dbad1eaa81804f7680cf389a77202ac9
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spelling	oai:doaj.org-article:dbad1eaa81804f7680cf389a77202ac92021-11-17T14:21:57ZEnhanced production and biochemical characterization of a thermostable amylase from thermophilic bacterium Geobacillus icigianus BITSNS0381658-365510.1080/16583655.2021.2002549https://doaj.org/article/dbad1eaa81804f7680cf389a77202ac92021-01-01T00:00:00Zhttp://dx.doi.org/10.1080/16583655.2021.2002549https://doaj.org/toc/1658-3655The current study focuses on optimizing, characterizing, and purifying a thermostable amylase from thermophilic bacteria Geobacillus icigianus (BITSNS038). The amylase was produced optimally at 18–24 h of incubation in the presence of starch and tryptone at 7.5 and 3.0 g/L, respectively (pH 7.0, 70°C, and 150 rpm). Km, Vmax values for starch were 2.17 mg/mL and 4.16 U/mL respectively. The enzyme showed excellent thermal stability at 70°C, retaining 62.5% residual activity after 8 h and a reduced deactivation rate constant (kd) of 0.001. When tested for its efficacy in starch hydrolysis, it showed 34.5% hydrolysis of corn starch slurry and antibiofilm activity, but no antimicrobial activity was noticed. The ultrafiltration led to partial purification of amylase showed 2.67-fold purification with a molecular weight in the range of 45 and 66 kDa. This is probably the first-ever report on a thermostable amylase from G. icigianus and its extensive potential uses.Snehi SoyVinod Kumar NigamShubha Rani SharmaTaylor & Francis Grouparticlegeobacilluskinetic modelingsaccharificationthermostable amylaseultrafiltrationScience (General)Q1-390ENJournal of Taibah University for Science, Vol 15, Iss 1, Pp 730-745 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	geobacillus kinetic modeling saccharification thermostable amylase ultrafiltration Science (General) Q1-390
spellingShingle	geobacillus kinetic modeling saccharification thermostable amylase ultrafiltration Science (General) Q1-390 Snehi Soy Vinod Kumar Nigam Shubha Rani Sharma Enhanced production and biochemical characterization of a thermostable amylase from thermophilic bacterium Geobacillus icigianus BITSNS038
description	The current study focuses on optimizing, characterizing, and purifying a thermostable amylase from thermophilic bacteria Geobacillus icigianus (BITSNS038). The amylase was produced optimally at 18–24 h of incubation in the presence of starch and tryptone at 7.5 and 3.0 g/L, respectively (pH 7.0, 70°C, and 150 rpm). Km, Vmax values for starch were 2.17 mg/mL and 4.16 U/mL respectively. The enzyme showed excellent thermal stability at 70°C, retaining 62.5% residual activity after 8 h and a reduced deactivation rate constant (kd) of 0.001. When tested for its efficacy in starch hydrolysis, it showed 34.5% hydrolysis of corn starch slurry and antibiofilm activity, but no antimicrobial activity was noticed. The ultrafiltration led to partial purification of amylase showed 2.67-fold purification with a molecular weight in the range of 45 and 66 kDa. This is probably the first-ever report on a thermostable amylase from G. icigianus and its extensive potential uses.
format	article
author	Snehi Soy Vinod Kumar Nigam Shubha Rani Sharma
author_facet	Snehi Soy Vinod Kumar Nigam Shubha Rani Sharma
author_sort	Snehi Soy
title	Enhanced production and biochemical characterization of a thermostable amylase from thermophilic bacterium Geobacillus icigianus BITSNS038
title_short	Enhanced production and biochemical characterization of a thermostable amylase from thermophilic bacterium Geobacillus icigianus BITSNS038
title_full	Enhanced production and biochemical characterization of a thermostable amylase from thermophilic bacterium Geobacillus icigianus BITSNS038
title_fullStr	Enhanced production and biochemical characterization of a thermostable amylase from thermophilic bacterium Geobacillus icigianus BITSNS038
title_full_unstemmed	Enhanced production and biochemical characterization of a thermostable amylase from thermophilic bacterium Geobacillus icigianus BITSNS038
title_sort	enhanced production and biochemical characterization of a thermostable amylase from thermophilic bacterium geobacillus icigianus bitsns038
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/dbad1eaa81804f7680cf389a77202ac9
work_keys_str_mv	AT snehisoy enhancedproductionandbiochemicalcharacterizationofathermostableamylasefromthermophilicbacteriumgeobacillusicigianusbitsns038 AT vinodkumarnigam enhancedproductionandbiochemicalcharacterizationofathermostableamylasefromthermophilicbacteriumgeobacillusicigianusbitsns038 AT shubharanisharma enhancedproductionandbiochemicalcharacterizationofathermostableamylasefromthermophilicbacteriumgeobacillusicigianusbitsns038
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Enhanced production and biochemical characterization of a thermostable amylase from thermophilic bacterium Geobacillus icigianus BITSNS038

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