Addition of Vegetable Oil to Improve Triterpenoids Production in Liquid Fermentation of Medicinal Fungus <i>Antrodia cinnamomea</i>

The liquid fermentation of <i>Antrodia cinnamomea</i> is a promising alternative source for fungus production compared to the wildly grown fruiting body. Elicitation is a strong tool to enhance the productivity in microbial cells to obtain more compounds of interest. In this study, in or...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Linghui Meng, Biaobiao Luo, Yang Yang, Mohammad Omar Faruque, Jiuliang Zhang, Xiaohua Li, Xuebo Hu
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Antrodia cinnamomea</i> submerged mycelium biomass triterpenoids liquid fermentation Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/dbaf922eecd541c4b8ad87dc62bc565f
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:dbaf922eecd541c4b8ad87dc62bc565f
record_format	dspace
spelling	oai:doaj.org-article:dbaf922eecd541c4b8ad87dc62bc565f2021-11-25T18:05:50ZAddition of Vegetable Oil to Improve Triterpenoids Production in Liquid Fermentation of Medicinal Fungus <i>Antrodia cinnamomea</i>10.3390/jof71109262309-608Xhttps://doaj.org/article/dbaf922eecd541c4b8ad87dc62bc565f2021-10-01T00:00:00Zhttps://www.mdpi.com/2309-608X/7/11/926https://doaj.org/toc/2309-608XThe liquid fermentation of <i>Antrodia cinnamomea</i> is a promising alternative source for fungus production compared to the wildly grown fruiting body. Elicitation is a strong tool to enhance the productivity in microbial cells to obtain more compounds of interest. In this study, in order to improve the fungus growth and its terpenoids production, various vegetable oils were added in the fermentation broth of <i>A. cinnamomea</i>. It was found that corn oil from a group of vegetable oils exhibited the best effect on the biomass and triterpenoid content. After optimization, the initial addition of 1% (<i>v</i>/<i>v</i>) corn oil plus the inoculation of 10% (<i>v</i>/<i>v</i>) mycelia led to a maximum triterpenoid yield (532.3 mg L<sup>−1</sup>), which was increased as much as fourfold compared to the blank control. Differential transcriptome analysis demonstrated that corn oil significantly enriched several metabolic pathways including glycolysis/gluconeogenesis, propanoate metabolism and transmembrane hydrophobins. The enriched pathways interacted with deferentially expressed genes (DEGs) induced by corn oil treatment. Our research provides a potential strategy for the large production of triterpenoids by the improved fermentation of <i>A. cinnamomea</i>.Linghui MengBiaobiao LuoYang YangMohammad Omar FaruqueJiuliang ZhangXiaohua LiXuebo HuMDPI AGarticle<i>Antrodia cinnamomea</i>submerged myceliumbiomasstriterpenoidsliquid fermentationBiology (General)QH301-705.5ENJournal of Fungi, Vol 7, Iss 926, p 926 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Antrodia cinnamomea</i> submerged mycelium biomass triterpenoids liquid fermentation Biology (General) QH301-705.5
spellingShingle	<i>Antrodia cinnamomea</i> submerged mycelium biomass triterpenoids liquid fermentation Biology (General) QH301-705.5 Linghui Meng Biaobiao Luo Yang Yang Mohammad Omar Faruque Jiuliang Zhang Xiaohua Li Xuebo Hu Addition of Vegetable Oil to Improve Triterpenoids Production in Liquid Fermentation of Medicinal Fungus <i>Antrodia cinnamomea</i>
description	The liquid fermentation of <i>Antrodia cinnamomea</i> is a promising alternative source for fungus production compared to the wildly grown fruiting body. Elicitation is a strong tool to enhance the productivity in microbial cells to obtain more compounds of interest. In this study, in order to improve the fungus growth and its terpenoids production, various vegetable oils were added in the fermentation broth of <i>A. cinnamomea</i>. It was found that corn oil from a group of vegetable oils exhibited the best effect on the biomass and triterpenoid content. After optimization, the initial addition of 1% (<i>v</i>/<i>v</i>) corn oil plus the inoculation of 10% (<i>v</i>/<i>v</i>) mycelia led to a maximum triterpenoid yield (532.3 mg L<sup>−1</sup>), which was increased as much as fourfold compared to the blank control. Differential transcriptome analysis demonstrated that corn oil significantly enriched several metabolic pathways including glycolysis/gluconeogenesis, propanoate metabolism and transmembrane hydrophobins. The enriched pathways interacted with deferentially expressed genes (DEGs) induced by corn oil treatment. Our research provides a potential strategy for the large production of triterpenoids by the improved fermentation of <i>A. cinnamomea</i>.
format	article
author	Linghui Meng Biaobiao Luo Yang Yang Mohammad Omar Faruque Jiuliang Zhang Xiaohua Li Xuebo Hu
author_facet	Linghui Meng Biaobiao Luo Yang Yang Mohammad Omar Faruque Jiuliang Zhang Xiaohua Li Xuebo Hu
author_sort	Linghui Meng
title	Addition of Vegetable Oil to Improve Triterpenoids Production in Liquid Fermentation of Medicinal Fungus <i>Antrodia cinnamomea</i>
title_short	Addition of Vegetable Oil to Improve Triterpenoids Production in Liquid Fermentation of Medicinal Fungus <i>Antrodia cinnamomea</i>
title_full	Addition of Vegetable Oil to Improve Triterpenoids Production in Liquid Fermentation of Medicinal Fungus <i>Antrodia cinnamomea</i>
title_fullStr	Addition of Vegetable Oil to Improve Triterpenoids Production in Liquid Fermentation of Medicinal Fungus <i>Antrodia cinnamomea</i>
title_full_unstemmed	Addition of Vegetable Oil to Improve Triterpenoids Production in Liquid Fermentation of Medicinal Fungus <i>Antrodia cinnamomea</i>
title_sort	addition of vegetable oil to improve triterpenoids production in liquid fermentation of medicinal fungus <i>antrodia cinnamomea</i>
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/dbaf922eecd541c4b8ad87dc62bc565f
work_keys_str_mv	AT linghuimeng additionofvegetableoiltoimprovetriterpenoidsproductioninliquidfermentationofmedicinalfungusiantrodiacinnamomeai AT biaobiaoluo additionofvegetableoiltoimprovetriterpenoidsproductioninliquidfermentationofmedicinalfungusiantrodiacinnamomeai AT yangyang additionofvegetableoiltoimprovetriterpenoidsproductioninliquidfermentationofmedicinalfungusiantrodiacinnamomeai AT mohammadomarfaruque additionofvegetableoiltoimprovetriterpenoidsproductioninliquidfermentationofmedicinalfungusiantrodiacinnamomeai AT jiuliangzhang additionofvegetableoiltoimprovetriterpenoidsproductioninliquidfermentationofmedicinalfungusiantrodiacinnamomeai AT xiaohuali additionofvegetableoiltoimprovetriterpenoidsproductioninliquidfermentationofmedicinalfungusiantrodiacinnamomeai AT xuebohu additionofvegetableoiltoimprovetriterpenoidsproductioninliquidfermentationofmedicinalfungusiantrodiacinnamomeai
_version_	1718411649494286336

Addition of Vegetable Oil to Improve Triterpenoids Production in Liquid Fermentation of Medicinal Fungus <i>Antrodia cinnamomea</i>

Ejemplares similares