Study of hepatoprotective effect of bearberry leaves extract under insulin resistance in rats

The aim of our study was to evaluate the antidiabetic and hepatoprotective efficacy of dry extract from bearberry leaves enriched with arginine in dexamethasone induced IR. Materials and methods. IR was induced in rats by low dose intraperitoneally injections of dexamethasone. Dexamethasone-induc...

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Autores principales:	Matar Mazen, Ganna Kravchenko, Oksana Krasilnikova
Formato:	article
Lenguaje:	EN
Publicado:	Scientific Route OÜ 2021
Materias:	insulin resistance diabetes mellitus type 2 hepatoprotection bearberry polyphenols arginine Medicine R
Acceso en línea:	https://doaj.org/article/dbbefeb5e6bb4c8895c2c3e09e32149c
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spelling	oai:doaj.org-article:dbbefeb5e6bb4c8895c2c3e09e32149c2021-12-03T07:43:49ZStudy of hepatoprotective effect of bearberry leaves extract under insulin resistance in rats2504-56602504-567910.21303/2504-5679.2021.002174https://doaj.org/article/dbbefeb5e6bb4c8895c2c3e09e32149c2021-11-01T00:00:00Zhttp://journal.eu-jr.eu/health/article/view/2174https://doaj.org/toc/2504-5660https://doaj.org/toc/2504-5679The aim of our study was to evaluate the antidiabetic and hepatoprotective efficacy of dry extract from bearberry leaves enriched with arginine in dexamethasone induced IR. Materials and methods. IR was induced in rats by low dose intraperitoneally injections of dexamethasone. Dexamethasone-induced IR in rats was treated by bearberry leaves extract enriched with arginine. Thus, animals were randomized into several groups including intact animals and animals, which administered reference compounds and medications. The activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST), gamma-glutamine transferase (GGT) were determined in blood serum and liver homogenate, in addition, in blood serum we measured lactate dehydrogenase (LDH) activity and lactate level and glycogen content liver tissue. Also, for the purpose of our experiment, in liver tissue were determined: thiobarbituric acid reactive substances (TBARS), diene conjugates (DC), and reduced glutathione (GSH) content; and superoxide dismutase (SOD), glutathione peroxidase (Gpx), and catalase (CAT) activities. All indices were determined using generally accepted unified methods or commercially available kits. Results. Long-term dexamethasone administration led to an increase in AST, ALT and GGT overall activity in the liver homogenate and serum; this could be the result of increased permeability of hepatocyte plasma membranes, as well as their enhanced synthesis in the liver. Studied extract ameliorate these indices of liver injury. Evaluation of indices that reflected oxidative stress and the antioxidant system status in liver confirmed oxidative stress development in IR rats` liver. Administration of arginine enriched bearberry leaves extract decrease TBARS and DC content in liver tissue, at the same time, improve SOD, Gpx, and CAT activities and increase GSH content. Conclusions. Bearberry leaves dry extract enriched with arginine inhibit oxidative stress development, improve membrane integrity, and normalize some indices of carbohydrate metabolism, particularly glycogen content in liver and lactate level in blood.Matar MazenGanna KravchenkoOksana KrasilnikovaScientific Route OÜarticleinsulin resistancediabetes mellitus type 2hepatoprotectionbearberrypolyphenolsarginineMedicineRENEUREKA: Health Sciences, Iss 6, Pp 48-53 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	insulin resistance diabetes mellitus type 2 hepatoprotection bearberry polyphenols arginine Medicine R
spellingShingle	insulin resistance diabetes mellitus type 2 hepatoprotection bearberry polyphenols arginine Medicine R Matar Mazen Ganna Kravchenko Oksana Krasilnikova Study of hepatoprotective effect of bearberry leaves extract under insulin resistance in rats
description	The aim of our study was to evaluate the antidiabetic and hepatoprotective efficacy of dry extract from bearberry leaves enriched with arginine in dexamethasone induced IR. Materials and methods. IR was induced in rats by low dose intraperitoneally injections of dexamethasone. Dexamethasone-induced IR in rats was treated by bearberry leaves extract enriched with arginine. Thus, animals were randomized into several groups including intact animals and animals, which administered reference compounds and medications. The activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST), gamma-glutamine transferase (GGT) were determined in blood serum and liver homogenate, in addition, in blood serum we measured lactate dehydrogenase (LDH) activity and lactate level and glycogen content liver tissue. Also, for the purpose of our experiment, in liver tissue were determined: thiobarbituric acid reactive substances (TBARS), diene conjugates (DC), and reduced glutathione (GSH) content; and superoxide dismutase (SOD), glutathione peroxidase (Gpx), and catalase (CAT) activities. All indices were determined using generally accepted unified methods or commercially available kits. Results. Long-term dexamethasone administration led to an increase in AST, ALT and GGT overall activity in the liver homogenate and serum; this could be the result of increased permeability of hepatocyte plasma membranes, as well as their enhanced synthesis in the liver. Studied extract ameliorate these indices of liver injury. Evaluation of indices that reflected oxidative stress and the antioxidant system status in liver confirmed oxidative stress development in IR rats` liver. Administration of arginine enriched bearberry leaves extract decrease TBARS and DC content in liver tissue, at the same time, improve SOD, Gpx, and CAT activities and increase GSH content. Conclusions. Bearberry leaves dry extract enriched with arginine inhibit oxidative stress development, improve membrane integrity, and normalize some indices of carbohydrate metabolism, particularly glycogen content in liver and lactate level in blood.
format	article
author	Matar Mazen Ganna Kravchenko Oksana Krasilnikova
author_facet	Matar Mazen Ganna Kravchenko Oksana Krasilnikova
author_sort	Matar Mazen
title	Study of hepatoprotective effect of bearberry leaves extract under insulin resistance in rats
title_short	Study of hepatoprotective effect of bearberry leaves extract under insulin resistance in rats
title_full	Study of hepatoprotective effect of bearberry leaves extract under insulin resistance in rats
title_fullStr	Study of hepatoprotective effect of bearberry leaves extract under insulin resistance in rats
title_full_unstemmed	Study of hepatoprotective effect of bearberry leaves extract under insulin resistance in rats
title_sort	study of hepatoprotective effect of bearberry leaves extract under insulin resistance in rats
publisher	Scientific Route OÜ
publishDate	2021
url	https://doaj.org/article/dbbefeb5e6bb4c8895c2c3e09e32149c
work_keys_str_mv	AT matarmazen studyofhepatoprotectiveeffectofbearberryleavesextractunderinsulinresistanceinrats AT gannakravchenko studyofhepatoprotectiveeffectofbearberryleavesextractunderinsulinresistanceinrats AT oksanakrasilnikova studyofhepatoprotectiveeffectofbearberryleavesextractunderinsulinresistanceinrats
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Study of hepatoprotective effect of bearberry leaves extract under insulin resistance in rats

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