In vitro study of sodium butyrate on soyasaponin challenged intestinal epithelial cells of turbot (Scophthalmus maximus L.) refer to inflammation, apoptosis and antioxidant enzymes

The study is aimed to investigate the protective effect and potential mechanisms of sodium butyrate (NaBT) on soyasaponins (SA) induced intestinal epithelial cells (IECs) injury in vitro. The primary IECs of turbot were developed and treated with 0.4, 1 and 4 mM NaBT in the presence of 0.4 mg/mL SA...

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Autores principales:	Guijuan Yu, Weihao Ou, Qinghui Ai, Wenbing Zhang, Kangsen Mai, Yanjiao Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Sodium butyrate Soyasaponins Inflammation Apoptosis MAPKs Turbot Zoology QL1-991
Acceso en línea:	https://doaj.org/article/dbc1f0afb0844a518edd9a54047e9bd6
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spelling	oai:doaj.org-article:dbc1f0afb0844a518edd9a54047e9bd62021-11-04T04:43:56ZIn vitro study of sodium butyrate on soyasaponin challenged intestinal epithelial cells of turbot (Scophthalmus maximus L.) refer to inflammation, apoptosis and antioxidant enzymes2667-011910.1016/j.fsirep.2021.100031https://doaj.org/article/dbc1f0afb0844a518edd9a54047e9bd62021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2667011921000268https://doaj.org/toc/2667-0119The study is aimed to investigate the protective effect and potential mechanisms of sodium butyrate (NaBT) on soyasaponins (SA) induced intestinal epithelial cells (IECs) injury in vitro. The primary IECs of turbot were developed and treated with 0.4, 1 and 4 mM NaBT in the presence of 0.4 mg/mL SA for 6 h to explore the protective effects of NaBT. The results showed that the addition of NaBT significantly down-regulated gene expression of inflammatory cytokine TNF-α, IL-1β and IL-8, pro-apoptosis relevant gene BAX, caspase-3, caspase-7 and caspase-9 induced by SA, while up-regulated anti-apoptosis gene Bcl-2. SA stimulation did not induce reactive oxygen species production, but elevated gene expression of antioxidant enzyme heme oxygenase-1 and superoxide dismutase. Moreover, the gene expression of those antioxidant enzyme was further up-regulated in NaBT groups. Furthermore, NaBT supplementation decreased the acid phosphatase and alkaline phosphatase activities and suppressed phosphorylation of p38 and c-Jun N-terminal kinase (JNK). In conclusion, NaBT could mitigate SA-induced inflammation and apoptosis and elevate gene expression of antioxidant enzymes on IECs of turbot and p38 and JNK signaling pathway participated in those processes.Guijuan YuWeihao OuQinghui AiWenbing ZhangKangsen MaiYanjiao ZhangElsevierarticleSodium butyrateSoyasaponinsInflammationApoptosisMAPKsTurbotZoologyQL1-991ENFish and Shellfish Immunology Reports, Vol 2, Iss , Pp 100031- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Sodium butyrate Soyasaponins Inflammation Apoptosis MAPKs Turbot Zoology QL1-991
spellingShingle	Sodium butyrate Soyasaponins Inflammation Apoptosis MAPKs Turbot Zoology QL1-991 Guijuan Yu Weihao Ou Qinghui Ai Wenbing Zhang Kangsen Mai Yanjiao Zhang In vitro study of sodium butyrate on soyasaponin challenged intestinal epithelial cells of turbot (Scophthalmus maximus L.) refer to inflammation, apoptosis and antioxidant enzymes
description	The study is aimed to investigate the protective effect and potential mechanisms of sodium butyrate (NaBT) on soyasaponins (SA) induced intestinal epithelial cells (IECs) injury in vitro. The primary IECs of turbot were developed and treated with 0.4, 1 and 4 mM NaBT in the presence of 0.4 mg/mL SA for 6 h to explore the protective effects of NaBT. The results showed that the addition of NaBT significantly down-regulated gene expression of inflammatory cytokine TNF-α, IL-1β and IL-8, pro-apoptosis relevant gene BAX, caspase-3, caspase-7 and caspase-9 induced by SA, while up-regulated anti-apoptosis gene Bcl-2. SA stimulation did not induce reactive oxygen species production, but elevated gene expression of antioxidant enzyme heme oxygenase-1 and superoxide dismutase. Moreover, the gene expression of those antioxidant enzyme was further up-regulated in NaBT groups. Furthermore, NaBT supplementation decreased the acid phosphatase and alkaline phosphatase activities and suppressed phosphorylation of p38 and c-Jun N-terminal kinase (JNK). In conclusion, NaBT could mitigate SA-induced inflammation and apoptosis and elevate gene expression of antioxidant enzymes on IECs of turbot and p38 and JNK signaling pathway participated in those processes.
format	article
author	Guijuan Yu Weihao Ou Qinghui Ai Wenbing Zhang Kangsen Mai Yanjiao Zhang
author_facet	Guijuan Yu Weihao Ou Qinghui Ai Wenbing Zhang Kangsen Mai Yanjiao Zhang
author_sort	Guijuan Yu
title	In vitro study of sodium butyrate on soyasaponin challenged intestinal epithelial cells of turbot (Scophthalmus maximus L.) refer to inflammation, apoptosis and antioxidant enzymes
title_short	In vitro study of sodium butyrate on soyasaponin challenged intestinal epithelial cells of turbot (Scophthalmus maximus L.) refer to inflammation, apoptosis and antioxidant enzymes
title_full	In vitro study of sodium butyrate on soyasaponin challenged intestinal epithelial cells of turbot (Scophthalmus maximus L.) refer to inflammation, apoptosis and antioxidant enzymes
title_fullStr	In vitro study of sodium butyrate on soyasaponin challenged intestinal epithelial cells of turbot (Scophthalmus maximus L.) refer to inflammation, apoptosis and antioxidant enzymes
title_full_unstemmed	In vitro study of sodium butyrate on soyasaponin challenged intestinal epithelial cells of turbot (Scophthalmus maximus L.) refer to inflammation, apoptosis and antioxidant enzymes
title_sort	in vitro study of sodium butyrate on soyasaponin challenged intestinal epithelial cells of turbot (scophthalmus maximus l.) refer to inflammation, apoptosis and antioxidant enzymes
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/dbc1f0afb0844a518edd9a54047e9bd6
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In vitro study of sodium butyrate on soyasaponin challenged intestinal epithelial cells of turbot (Scophthalmus maximus L.) refer to inflammation, apoptosis and antioxidant enzymes

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