Elisa Method For Serum Hepcidin Quantification In Bulgarian Population

Hepcidin is a 25-aminoacid cysteine-rich iron regulating peptide. Hepcidin quantification in human blood may provide further insights for the pathogenesis of disorders of iron homeostasis and might prove a valuable tool for clinicians for the differential diagnosis of anaemia. This study describes E...

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Autores principales:	Manolov V., Atanasova B., Vasilev V., Tzatchev K., Velizarova M.
Formato:	article
Lenguaje:	EN
Publicado:	Sciendo 2014
Materias:	hepcidin quantification elisa hemodialysis iron metabolism Medicine R
Acceso en línea:	https://doaj.org/article/dbc2722631b04b03a6e4a28f688103be
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spelling	oai:doaj.org-article:dbc2722631b04b03a6e4a28f688103be2021-12-02T14:59:04ZElisa Method For Serum Hepcidin Quantification In Bulgarian Population0324-175010.2478/amb-2014-0003https://doaj.org/article/dbc2722631b04b03a6e4a28f688103be2014-06-01T00:00:00Zhttps://doi.org/10.2478/amb-2014-0003https://doaj.org/toc/0324-1750Hepcidin is a 25-aminoacid cysteine-rich iron regulating peptide. Hepcidin quantification in human blood may provide further insights for the pathogenesis of disorders of iron homeostasis and might prove a valuable tool for clinicians for the differential diagnosis of anaemia. This study describes ELISA immunoassay for hepcidin quantification in human serum. We used a sandwich ELISA method from USCN Life Science inc., that consists of ready to use, pre-coated 96-well strip plate with 2 antihepcidin-25 monoclonal antibodies. A recombinant hepcidin in 16 μg/l concentration is used as a standard; it reconstitutes with 1.0 ml standard diluent to prepare a stock solution. We correlated ELISA results of hepcidin-25 measurements in healthy population with hemodialysis patients. The sandwich ELISA was highly specific for hepcidin-25, having a low limit of quantification of 0.020 μg/l. Hepcidin- 25 concentrations were increased in hemodialysis patients (median 33.05 μg/l, range 22.31 -60.98 μg/l, n = 10) compared with healthy individuals (median 12.41 μg/l, range 6.05-18.53 μg/l, n = 40). The use of 2 monoclonal antibodies in a sandwich ELISA format provides a robust, convenient and not very expensive method for measuring concentrations of the active form of hepcidin. It should help to improve our understanding of the role of hepcidin in regulating iron metabolism.Manolov V.Atanasova B.Vasilev V.Tzatchev K.Velizarova M.Sciendoarticlehepcidinquantificationelisahemodialysisiron metabolismMedicineRENActa Medica Bulgarica, Vol 41, Iss 1, Pp 22-29 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	hepcidin quantification elisa hemodialysis iron metabolism Medicine R
spellingShingle	hepcidin quantification elisa hemodialysis iron metabolism Medicine R Manolov V. Atanasova B. Vasilev V. Tzatchev K. Velizarova M. Elisa Method For Serum Hepcidin Quantification In Bulgarian Population
description	Hepcidin is a 25-aminoacid cysteine-rich iron regulating peptide. Hepcidin quantification in human blood may provide further insights for the pathogenesis of disorders of iron homeostasis and might prove a valuable tool for clinicians for the differential diagnosis of anaemia. This study describes ELISA immunoassay for hepcidin quantification in human serum. We used a sandwich ELISA method from USCN Life Science inc., that consists of ready to use, pre-coated 96-well strip plate with 2 antihepcidin-25 monoclonal antibodies. A recombinant hepcidin in 16 μg/l concentration is used as a standard; it reconstitutes with 1.0 ml standard diluent to prepare a stock solution. We correlated ELISA results of hepcidin-25 measurements in healthy population with hemodialysis patients. The sandwich ELISA was highly specific for hepcidin-25, having a low limit of quantification of 0.020 μg/l. Hepcidin- 25 concentrations were increased in hemodialysis patients (median 33.05 μg/l, range 22.31 -60.98 μg/l, n = 10) compared with healthy individuals (median 12.41 μg/l, range 6.05-18.53 μg/l, n = 40). The use of 2 monoclonal antibodies in a sandwich ELISA format provides a robust, convenient and not very expensive method for measuring concentrations of the active form of hepcidin. It should help to improve our understanding of the role of hepcidin in regulating iron metabolism.
format	article
author	Manolov V. Atanasova B. Vasilev V. Tzatchev K. Velizarova M.
author_facet	Manolov V. Atanasova B. Vasilev V. Tzatchev K. Velizarova M.
author_sort	Manolov V.
title	Elisa Method For Serum Hepcidin Quantification In Bulgarian Population
title_short	Elisa Method For Serum Hepcidin Quantification In Bulgarian Population
title_full	Elisa Method For Serum Hepcidin Quantification In Bulgarian Population
title_fullStr	Elisa Method For Serum Hepcidin Quantification In Bulgarian Population
title_full_unstemmed	Elisa Method For Serum Hepcidin Quantification In Bulgarian Population
title_sort	elisa method for serum hepcidin quantification in bulgarian population
publisher	Sciendo
publishDate	2014
url	https://doaj.org/article/dbc2722631b04b03a6e4a28f688103be
work_keys_str_mv	AT manolovv elisamethodforserumhepcidinquantificationinbulgarianpopulation AT atanasovab elisamethodforserumhepcidinquantificationinbulgarianpopulation AT vasilevv elisamethodforserumhepcidinquantificationinbulgarianpopulation AT tzatchevk elisamethodforserumhepcidinquantificationinbulgarianpopulation AT velizarovam elisamethodforserumhepcidinquantificationinbulgarianpopulation
_version_	1718389232430481408

Elisa Method For Serum Hepcidin Quantification In Bulgarian Population

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