Live imaging and quantitative analysis of Aspergillus fumigatus growth and morphology during inter-microbial interaction with Pseudomonas aeruginosa

Pseudomonas aeruginosa (PA) and Aspergillus fumigatus (AF) chronically colonize the airways of patients with cystic fibrosis or chronic immunosuppression and mutually affect each other’s pathogenesis. Here, we evaluated IncuCyte time-lapse imaging and NeuroTrackTM (NT) analysis (Wurster et al., 2019...

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Autores principales:	Sebastian Wurster, Gabriele Sass, Nathaniel D. Albert, Hasan Nazik, Eric Déziel, David A. Stevens, Dimitrios P. Kontoyiannis
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2020
Materias:	mixed infection intermicrobial interaction live imaging morphogenesis iron metabolism pseudomonas aspergillus Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/dbc320d2fe6d4e02b5f03d0674e6d4ec
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spelling	oai:doaj.org-article:dbc320d2fe6d4e02b5f03d0674e6d4ec2021-11-17T14:21:59ZLive imaging and quantitative analysis of Aspergillus fumigatus growth and morphology during inter-microbial interaction with Pseudomonas aeruginosa2150-55942150-560810.1080/21505594.2020.1827885https://doaj.org/article/dbc320d2fe6d4e02b5f03d0674e6d4ec2020-12-01T00:00:00Zhttp://dx.doi.org/10.1080/21505594.2020.1827885https://doaj.org/toc/2150-5594https://doaj.org/toc/2150-5608Pseudomonas aeruginosa (PA) and Aspergillus fumigatus (AF) chronically colonize the airways of patients with cystic fibrosis or chronic immunosuppression and mutually affect each other’s pathogenesis. Here, we evaluated IncuCyte time-lapse imaging and NeuroTrackTM (NT) analysis (Wurster et al., 2019, mBio) as a toolbox to study mycelial expansion and morphogenesis of AF during interaction with PA. Co-incubation of AF with supernatant filtrates of wild-type (WT) PA strains strongly inhibited hyphal growth and branching. Consonant with prior metabolic studies, pyoverdine-deficient PA mutants had significantly attenuated inhibitory capacity. Accordingly, purified PA products pyoverdine and pyocyanin suppressed mycelial expansion of AF in a concentration-dependent way. Using fluorescence-guided tracking of GFP-AF293 mycelia during co-culture with live WT PA cells, we found significant inoculum-dependent mycelial growth inhibition and robust precision of the NT algorithm. Collectively, our experiments position IncuCyte NT as an efficient platform for longitudinal analysis of fungal growth and morphogenesis during bacterial co-infection.Sebastian WursterGabriele SassNathaniel D. AlbertHasan NazikEric DézielDavid A. StevensDimitrios P. KontoyiannisTaylor & Francis Grouparticlemixed infectionintermicrobial interactionlive imagingmorphogenesisiron metabolismpseudomonasaspergillusInfectious and parasitic diseasesRC109-216ENVirulence, Vol 11, Iss 1, Pp 1329-1336 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	mixed infection intermicrobial interaction live imaging morphogenesis iron metabolism pseudomonas aspergillus Infectious and parasitic diseases RC109-216
spellingShingle	mixed infection intermicrobial interaction live imaging morphogenesis iron metabolism pseudomonas aspergillus Infectious and parasitic diseases RC109-216 Sebastian Wurster Gabriele Sass Nathaniel D. Albert Hasan Nazik Eric Déziel David A. Stevens Dimitrios P. Kontoyiannis Live imaging and quantitative analysis of Aspergillus fumigatus growth and morphology during inter-microbial interaction with Pseudomonas aeruginosa
description	Pseudomonas aeruginosa (PA) and Aspergillus fumigatus (AF) chronically colonize the airways of patients with cystic fibrosis or chronic immunosuppression and mutually affect each other’s pathogenesis. Here, we evaluated IncuCyte time-lapse imaging and NeuroTrackTM (NT) analysis (Wurster et al., 2019, mBio) as a toolbox to study mycelial expansion and morphogenesis of AF during interaction with PA. Co-incubation of AF with supernatant filtrates of wild-type (WT) PA strains strongly inhibited hyphal growth and branching. Consonant with prior metabolic studies, pyoverdine-deficient PA mutants had significantly attenuated inhibitory capacity. Accordingly, purified PA products pyoverdine and pyocyanin suppressed mycelial expansion of AF in a concentration-dependent way. Using fluorescence-guided tracking of GFP-AF293 mycelia during co-culture with live WT PA cells, we found significant inoculum-dependent mycelial growth inhibition and robust precision of the NT algorithm. Collectively, our experiments position IncuCyte NT as an efficient platform for longitudinal analysis of fungal growth and morphogenesis during bacterial co-infection.
format	article
author	Sebastian Wurster Gabriele Sass Nathaniel D. Albert Hasan Nazik Eric Déziel David A. Stevens Dimitrios P. Kontoyiannis
author_facet	Sebastian Wurster Gabriele Sass Nathaniel D. Albert Hasan Nazik Eric Déziel David A. Stevens Dimitrios P. Kontoyiannis
author_sort	Sebastian Wurster
title	Live imaging and quantitative analysis of Aspergillus fumigatus growth and morphology during inter-microbial interaction with Pseudomonas aeruginosa
title_short	Live imaging and quantitative analysis of Aspergillus fumigatus growth and morphology during inter-microbial interaction with Pseudomonas aeruginosa
title_full	Live imaging and quantitative analysis of Aspergillus fumigatus growth and morphology during inter-microbial interaction with Pseudomonas aeruginosa
title_fullStr	Live imaging and quantitative analysis of Aspergillus fumigatus growth and morphology during inter-microbial interaction with Pseudomonas aeruginosa
title_full_unstemmed	Live imaging and quantitative analysis of Aspergillus fumigatus growth and morphology during inter-microbial interaction with Pseudomonas aeruginosa
title_sort	live imaging and quantitative analysis of aspergillus fumigatus growth and morphology during inter-microbial interaction with pseudomonas aeruginosa
publisher	Taylor & Francis Group
publishDate	2020
url	https://doaj.org/article/dbc320d2fe6d4e02b5f03d0674e6d4ec
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Live imaging and quantitative analysis of Aspergillus fumigatus growth and morphology during inter-microbial interaction with Pseudomonas aeruginosa

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