prox1b Activity is essential in zebrafish lymphangiogenesis.

<h4>Background</h4>The lymphatic vascular system, draining interstitial fluids from most tissues and organs, exerts crucial functions in several physiological and pathological processes. Lymphatic system development depends on Prox1, the first marker to be expressed in the endothelial ce...

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Autores principales:	Luca Del Giacco, Anna Pistocchi, Anna Ghilardi
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/dbcafad521ae48058971f2a5762e11ee
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spelling	oai:doaj.org-article:dbcafad521ae48058971f2a5762e11ee2021-11-18T07:03:10Zprox1b Activity is essential in zebrafish lymphangiogenesis.1932-620310.1371/journal.pone.0013170https://doaj.org/article/dbcafad521ae48058971f2a5762e11ee2010-10-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20976189/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>The lymphatic vascular system, draining interstitial fluids from most tissues and organs, exerts crucial functions in several physiological and pathological processes. Lymphatic system development depends on Prox1, the first marker to be expressed in the endothelial cells of the cardinal vein from where lymph vessels originate. Prox1 ortholog in the optically clear, easily manipulated zebrafish model has been previously isolated and its contribution to lymphangiogenesis has been clarified. Because of a round of genome duplication occurred at the base of teleosts radiation, several zebrafish genes have been retained in duplicate through evolution. We investigated for the presence of additional prox1 genes and determined their role in zebrafish lymphangiogenesis.<h4>Methodology/principal findings</h4>We isolated a second ortholog, named prox1b, and analyzed its expression during development by whole mount in situ hybridization (WISH). We detected strong prox1b expression in the endothelium of the posterior cardinal vein (PCV) from where lymphatic precursors originate. To analyze prox1b involvement in lymphangiogenesis we utilized the fli1:GFP transgenics and followed the formation of the toracic duct (TD), the primary lymph vessel in fish, after prox1b knockdown. Our findings clearly demonstrated that the absence of prox1b activity severely hampers the formation of the TD.<h4>Conclusions/significance</h4>This work provides substantial progress toward the understanding of zebrafish lymphangiogenesis. In light of the features shared by the lymphatic systems of zebrafish and higher vertebrates, the establishment of such lymphatic model will provide a powerful tool to study, for instance, disorders of body fluid homeostasis, inflammation and cancer metastasis, and may ultimately contribute to novel therapies.Luca Del GiaccoAnna PistocchiAnna GhilardiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 10, p e13170 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Luca Del Giacco Anna Pistocchi Anna Ghilardi prox1b Activity is essential in zebrafish lymphangiogenesis.
description	<h4>Background</h4>The lymphatic vascular system, draining interstitial fluids from most tissues and organs, exerts crucial functions in several physiological and pathological processes. Lymphatic system development depends on Prox1, the first marker to be expressed in the endothelial cells of the cardinal vein from where lymph vessels originate. Prox1 ortholog in the optically clear, easily manipulated zebrafish model has been previously isolated and its contribution to lymphangiogenesis has been clarified. Because of a round of genome duplication occurred at the base of teleosts radiation, several zebrafish genes have been retained in duplicate through evolution. We investigated for the presence of additional prox1 genes and determined their role in zebrafish lymphangiogenesis.<h4>Methodology/principal findings</h4>We isolated a second ortholog, named prox1b, and analyzed its expression during development by whole mount in situ hybridization (WISH). We detected strong prox1b expression in the endothelium of the posterior cardinal vein (PCV) from where lymphatic precursors originate. To analyze prox1b involvement in lymphangiogenesis we utilized the fli1:GFP transgenics and followed the formation of the toracic duct (TD), the primary lymph vessel in fish, after prox1b knockdown. Our findings clearly demonstrated that the absence of prox1b activity severely hampers the formation of the TD.<h4>Conclusions/significance</h4>This work provides substantial progress toward the understanding of zebrafish lymphangiogenesis. In light of the features shared by the lymphatic systems of zebrafish and higher vertebrates, the establishment of such lymphatic model will provide a powerful tool to study, for instance, disorders of body fluid homeostasis, inflammation and cancer metastasis, and may ultimately contribute to novel therapies.
format	article
author	Luca Del Giacco Anna Pistocchi Anna Ghilardi
author_facet	Luca Del Giacco Anna Pistocchi Anna Ghilardi
author_sort	Luca Del Giacco
title	prox1b Activity is essential in zebrafish lymphangiogenesis.
title_short	prox1b Activity is essential in zebrafish lymphangiogenesis.
title_full	prox1b Activity is essential in zebrafish lymphangiogenesis.
title_fullStr	prox1b Activity is essential in zebrafish lymphangiogenesis.
title_full_unstemmed	prox1b Activity is essential in zebrafish lymphangiogenesis.
title_sort	prox1b activity is essential in zebrafish lymphangiogenesis.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/dbcafad521ae48058971f2a5762e11ee
work_keys_str_mv	AT lucadelgiacco prox1bactivityisessentialinzebrafishlymphangiogenesis AT annapistocchi prox1bactivityisessentialinzebrafishlymphangiogenesis AT annaghilardi prox1bactivityisessentialinzebrafishlymphangiogenesis
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prox1b Activity is essential in zebrafish lymphangiogenesis.

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