A-FABP-PTEN/AKT Regulates Insulin Resistance in Preadipocyte Cell 3T3-L1 Cells

Rensiqin Wu,1 Hui Wang,1 Jian Huangfu,1 Rui Xiao2 1Department of Endocrinology, The Affiliated Hospital of Inner Mongolia Medical University, Hohhot, 010000, Inner Mongolia, People’s Republic of China; 2Key Laboratory of Molecular Pathology, Inner Mongolia Medical University, Huhhot, 01000...

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Autores principales:	Wu R, Wang H, Huangfu J, Xiao R
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	a-fabp pten akt preadipocyte differentiation insulin resistance Specialties of internal medicine RC581-951
Acceso en línea:	https://doaj.org/article/dbd3202791b143b786e65fb4664e2001
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spelling	oai:doaj.org-article:dbd3202791b143b786e65fb4664e20012021-12-02T16:58:10ZA-FABP-PTEN/AKT Regulates Insulin Resistance in Preadipocyte Cell 3T3-L1 Cells1178-7007https://doaj.org/article/dbd3202791b143b786e65fb4664e20012021-05-01T00:00:00Zhttps://www.dovepress.com/a-fabp-ptenakt-regulates-insulin-resistance-in-preadipocyte-cell-3t3-l-peer-reviewed-fulltext-article-DMSOhttps://doaj.org/toc/1178-7007Rensiqin Wu,1 Hui Wang,1 Jian Huangfu,1 Rui Xiao2 1Department of Endocrinology, The Affiliated Hospital of Inner Mongolia Medical University, Hohhot, 010000, Inner Mongolia, People’s Republic of China; 2Key Laboratory of Molecular Pathology, Inner Mongolia Medical University, Huhhot, 010000, Inner Mongolia, People’s Republic of ChinaCorrespondence: Jian HuangfuDepartment of Endocrinology, The Affiliated Hospital of Inner Mongolia Medical University, NO.1st Tunnel North Road, Hohhot, 010000, Inner Mongolia, People’s Republic of ChinaTel +86-18686053304Email hfj999dr@yeah.netRui XiaoKey Laboratory of Molecular Pathology, Inner Mongolia Medical University, NO.1st Tunnel North Road, Huhhot, 010000, Inner Mongolia, People’s Republic of ChinaTel +86-18686053304Email xiaorui79@21cn.comObjective: The purpose of this study was to explore the regulation of A-FABP-PTEN/AKT on insulin resistance in preadipocyte 3T3-L1 cell.Methods: siRNA interference method was used to knock-down the A-FABP expression in 3T3-L1 cells. The cell proliferation was detected by oil-O staining and MTT. The protein and mRNA expression levels of A-FABP, PTEN and AKT were detected by Western blot and qPCR.Results: Inhibition of A-FABP expression increased cell proliferation activity of the 3T3-L1 cells. Moreover, siRNA3 significantly reduced A-FABP mRNA expression compared with siRNA1 and siRNA2 (P< 0.05). The A-FABP mRNA level was significantly increased in the induced 3T3-L1 cells, while the PTEN mRNA expression was significantly decreased (P< 0.05). Inhibition of A-FABP can significantly increase the PTEN mRNA expression in the process of induced 3T3-L1 cells (P< 0.05). Overexpression of A-FABP can also increase the PTEN mRNA expression in the process of 3T3-L1 cell proliferation (P< 0.05). Furthermore, the protein expression levels of PTEN and p-AKT expression were not changed in the process of 3T3-L1 cell proliferation with or without A-FABP interference (P> 0.05). However, inhibition of A-FABP significantly increased the PTEN protein expression and reduced the p-AKT protein expression in the induced 3T3-L1 cells.Conclusion: Our finding suggested that A-FABP can directly inhibit the phosphorylation of AKT and increase the PTEN expression in the process of normal adipocyte differentiation, which speculated that A-FABP played a crucial role by adjusting the AKT activity in the process of adipocyte differentiation.Keywords: A-FABP, PTEN, AKT, preadipocyte differentiation, insulin resistanceWu RWang HHuangfu JXiao RDove Medical Pressarticlea-fabpptenaktpreadipocyte differentiationinsulin resistanceSpecialties of internal medicineRC581-951ENDiabetes, Metabolic Syndrome and Obesity: Targets and Therapy, Vol Volume 14, Pp 2103-2110 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	a-fabp pten akt preadipocyte differentiation insulin resistance Specialties of internal medicine RC581-951
spellingShingle	a-fabp pten akt preadipocyte differentiation insulin resistance Specialties of internal medicine RC581-951 Wu R Wang H Huangfu J Xiao R A-FABP-PTEN/AKT Regulates Insulin Resistance in Preadipocyte Cell 3T3-L1 Cells
description	Rensiqin Wu,1 Hui Wang,1 Jian Huangfu,1 Rui Xiao2 1Department of Endocrinology, The Affiliated Hospital of Inner Mongolia Medical University, Hohhot, 010000, Inner Mongolia, People’s Republic of China; 2Key Laboratory of Molecular Pathology, Inner Mongolia Medical University, Huhhot, 010000, Inner Mongolia, People’s Republic of ChinaCorrespondence: Jian HuangfuDepartment of Endocrinology, The Affiliated Hospital of Inner Mongolia Medical University, NO.1st Tunnel North Road, Hohhot, 010000, Inner Mongolia, People’s Republic of ChinaTel +86-18686053304Email hfj999dr@yeah.netRui XiaoKey Laboratory of Molecular Pathology, Inner Mongolia Medical University, NO.1st Tunnel North Road, Huhhot, 010000, Inner Mongolia, People’s Republic of ChinaTel +86-18686053304Email xiaorui79@21cn.comObjective: The purpose of this study was to explore the regulation of A-FABP-PTEN/AKT on insulin resistance in preadipocyte 3T3-L1 cell.Methods: siRNA interference method was used to knock-down the A-FABP expression in 3T3-L1 cells. The cell proliferation was detected by oil-O staining and MTT. The protein and mRNA expression levels of A-FABP, PTEN and AKT were detected by Western blot and qPCR.Results: Inhibition of A-FABP expression increased cell proliferation activity of the 3T3-L1 cells. Moreover, siRNA3 significantly reduced A-FABP mRNA expression compared with siRNA1 and siRNA2 (P< 0.05). The A-FABP mRNA level was significantly increased in the induced 3T3-L1 cells, while the PTEN mRNA expression was significantly decreased (P< 0.05). Inhibition of A-FABP can significantly increase the PTEN mRNA expression in the process of induced 3T3-L1 cells (P< 0.05). Overexpression of A-FABP can also increase the PTEN mRNA expression in the process of 3T3-L1 cell proliferation (P< 0.05). Furthermore, the protein expression levels of PTEN and p-AKT expression were not changed in the process of 3T3-L1 cell proliferation with or without A-FABP interference (P> 0.05). However, inhibition of A-FABP significantly increased the PTEN protein expression and reduced the p-AKT protein expression in the induced 3T3-L1 cells.Conclusion: Our finding suggested that A-FABP can directly inhibit the phosphorylation of AKT and increase the PTEN expression in the process of normal adipocyte differentiation, which speculated that A-FABP played a crucial role by adjusting the AKT activity in the process of adipocyte differentiation.Keywords: A-FABP, PTEN, AKT, preadipocyte differentiation, insulin resistance
format	article
author	Wu R Wang H Huangfu J Xiao R
author_facet	Wu R Wang H Huangfu J Xiao R
author_sort	Wu R
title	A-FABP-PTEN/AKT Regulates Insulin Resistance in Preadipocyte Cell 3T3-L1 Cells
title_short	A-FABP-PTEN/AKT Regulates Insulin Resistance in Preadipocyte Cell 3T3-L1 Cells
title_full	A-FABP-PTEN/AKT Regulates Insulin Resistance in Preadipocyte Cell 3T3-L1 Cells
title_fullStr	A-FABP-PTEN/AKT Regulates Insulin Resistance in Preadipocyte Cell 3T3-L1 Cells
title_full_unstemmed	A-FABP-PTEN/AKT Regulates Insulin Resistance in Preadipocyte Cell 3T3-L1 Cells
title_sort	a-fabp-pten/akt regulates insulin resistance in preadipocyte cell 3t3-l1 cells
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/dbd3202791b143b786e65fb4664e2001
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A-FABP-PTEN/AKT Regulates Insulin Resistance in Preadipocyte Cell 3T3-L1 Cells

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