Bulked segregant analysis reveals candidate genes responsible for dwarf formation in woody oilseed crop castor bean

Abstract Plant dwarfism is a desirable agronomic trait in non-timber trees, but little is known about the physiological and molecular mechanism underlying dwarfism in woody plants. Castor bean (Ricinus communis) is a typical woody oilseed crop. We performed cytological observations within xylem, phl...

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Autores principales:	Zaiqing Wang, Anmin Yu, Fei Li, Wei Xu, Bing Han, Xiaomao Cheng, Aizhong Liu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/dbd65b2dfdc0447c860481001e68b745
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spelling	oai:doaj.org-article:dbd65b2dfdc0447c860481001e68b7452021-12-02T13:17:42ZBulked segregant analysis reveals candidate genes responsible for dwarf formation in woody oilseed crop castor bean10.1038/s41598-021-85644-12045-2322https://doaj.org/article/dbd65b2dfdc0447c860481001e68b7452021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85644-1https://doaj.org/toc/2045-2322Abstract Plant dwarfism is a desirable agronomic trait in non-timber trees, but little is known about the physiological and molecular mechanism underlying dwarfism in woody plants. Castor bean (Ricinus communis) is a typical woody oilseed crop. We performed cytological observations within xylem, phloem and cambia tissues, revealing that divergent cell growth in all tissues might play a role in the dwarf phenotype in cultivated castor bean. Based on bulked segregant analyses for a F2 population generated from the crossing of a tall and a dwarf accession, we identified two QTLs associated with plant height, covering 325 candidate genes. One of these, Rc5NG4-1 encoding a putative IAA transport protein localized in the tonoplast was functionally characterized. A non-synonymous SNP (altering the amino acid sequence from Y to C at position 218) differentiated the tall and dwarf plants and we confirmed, through heterologous yeast transformation, that the IAA uptake capacities of Rc5NG4-1Y and Rc5NG4-1C were significantly different. This study provides insights into the physiological and molecular mechanisms of dwarfing in woody non-timber economically important plants, with potential to aid in the genetic breeding of castor bean and other related crops.Zaiqing WangAnmin YuFei LiWei XuBing HanXiaomao ChengAizhong LiuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Zaiqing Wang Anmin Yu Fei Li Wei Xu Bing Han Xiaomao Cheng Aizhong Liu Bulked segregant analysis reveals candidate genes responsible for dwarf formation in woody oilseed crop castor bean
description	Abstract Plant dwarfism is a desirable agronomic trait in non-timber trees, but little is known about the physiological and molecular mechanism underlying dwarfism in woody plants. Castor bean (Ricinus communis) is a typical woody oilseed crop. We performed cytological observations within xylem, phloem and cambia tissues, revealing that divergent cell growth in all tissues might play a role in the dwarf phenotype in cultivated castor bean. Based on bulked segregant analyses for a F2 population generated from the crossing of a tall and a dwarf accession, we identified two QTLs associated with plant height, covering 325 candidate genes. One of these, Rc5NG4-1 encoding a putative IAA transport protein localized in the tonoplast was functionally characterized. A non-synonymous SNP (altering the amino acid sequence from Y to C at position 218) differentiated the tall and dwarf plants and we confirmed, through heterologous yeast transformation, that the IAA uptake capacities of Rc5NG4-1Y and Rc5NG4-1C were significantly different. This study provides insights into the physiological and molecular mechanisms of dwarfing in woody non-timber economically important plants, with potential to aid in the genetic breeding of castor bean and other related crops.
format	article
author	Zaiqing Wang Anmin Yu Fei Li Wei Xu Bing Han Xiaomao Cheng Aizhong Liu
author_facet	Zaiqing Wang Anmin Yu Fei Li Wei Xu Bing Han Xiaomao Cheng Aizhong Liu
author_sort	Zaiqing Wang
title	Bulked segregant analysis reveals candidate genes responsible for dwarf formation in woody oilseed crop castor bean
title_short	Bulked segregant analysis reveals candidate genes responsible for dwarf formation in woody oilseed crop castor bean
title_full	Bulked segregant analysis reveals candidate genes responsible for dwarf formation in woody oilseed crop castor bean
title_fullStr	Bulked segregant analysis reveals candidate genes responsible for dwarf formation in woody oilseed crop castor bean
title_full_unstemmed	Bulked segregant analysis reveals candidate genes responsible for dwarf formation in woody oilseed crop castor bean
title_sort	bulked segregant analysis reveals candidate genes responsible for dwarf formation in woody oilseed crop castor bean
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/dbd65b2dfdc0447c860481001e68b745
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Bulked segregant analysis reveals candidate genes responsible for dwarf formation in woody oilseed crop castor bean

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