Comparison of Transcriptional Responses and Metabolic Alterations in Three Multidrug-Resistant Model Microorganisms, <named-content content-type="genus-species">Staphylococcus aureus</named-content> ATCC BAA-39, <named-content content-type="genus-species">Escherichia coli</named-content> ATCC BAA-196, and <named-content content-type="genus-species">Acinetobacter baumannii</named-content> ATCC BAA-1790, on Exposure to Iodine-Containing Nano-micelle Drug FS-1

ABSTRACT Iodine is one of the oldest antimicrobial agents. Until now, there have been no reports on acquiring resistance to iodine. Recent studies showed promising results on application of iodine-containing nano-micelles, FS-1, against antibiotic-resistant pathogens as a supplement to antibiotic th...

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Autores principales:	Ilya S. Korotetskiy, Sergey V. Shilov, Tatyana V. Kuznetsova, Aleksandr I. Ilin, Monique Joubert, Setshaba Taukobong, Oleg N. Reva
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2021
Materias:	transcriptomics Escherichia coli Staphylococcus aureus Acinetobacter baumannii antibiotic resistance iodine Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/dbd82ec33fd84895be58be61dad53bdc
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spelling	oai:doaj.org-article:dbd82ec33fd84895be58be61dad53bdc2021-12-02T19:22:27ZComparison of Transcriptional Responses and Metabolic Alterations in Three Multidrug-Resistant Model Microorganisms, <named-content content-type="genus-species">Staphylococcus aureus</named-content> ATCC BAA-39, <named-content content-type="genus-species">Escherichia coli</named-content> ATCC BAA-196, and <named-content content-type="genus-species">Acinetobacter baumannii</named-content> ATCC BAA-1790, on Exposure to Iodine-Containing Nano-micelle Drug FS-110.1128/mSystems.01293-202379-5077https://doaj.org/article/dbd82ec33fd84895be58be61dad53bdc2021-04-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSystems.01293-20https://doaj.org/toc/2379-5077ABSTRACT Iodine is one of the oldest antimicrobial agents. Until now, there have been no reports on acquiring resistance to iodine. Recent studies showed promising results on application of iodine-containing nano-micelles, FS-1, against antibiotic-resistant pathogens as a supplement to antibiotic therapy. The mechanisms of the action, however, remain unclear. The aim of this study was to perform a holistic analysis and comparison of gene regulation in three phylogenetically distant multidrug-resistant reference strains representing pathogens associated with nosocomial infections from the ATCC culture collection: Escherichia coli BAA-196, Staphylococcus aureus BAA-39, and Acinetobacter baumannii BAA-1790. These cultures were treated by a 5-min exposure to sublethal concentrations of the iodine-containing drug FS-1 applied in the late lagging phase and the middle of the logarithmic growth phase. Complete genome sequences of these strains were obtained in the previous studies. Gene regulation was studied by total RNA extraction and Ion Torrent sequencing followed by mapping the RNA reads against the reference genome sequences and statistical processing of read counts using the DESeq2 algorithm. It was found that the treatment of bacteria with FS-1 profoundly affected the expression of many genes involved in the central metabolic pathways; however, alterations of the gene expression profiles were species specific and depended on the growth phase. Disruption of respiratory electron transfer membrane complexes, increased penetrability of bacterial cell walls, and osmotic and oxidative stresses leading to DNA damage were the major factors influencing the treated bacteria. IMPORTANCE Infections caused by antibiotic-resistant bacteria threaten public health worldwide. Combinatorial therapy in which antibiotics are administered together with supplementary drugs improving susceptibility of pathogens to the regular antibiotics is considered a promising way to overcome this problem. An induction of antibiotic resistance reversion by the iodine-containing nano-micelle drug FS-1 has been reported recently. This drug is currently under clinical trials in Kazakhstan against multidrug-resistant tuberculosis. The effects of released iodine on metabolic and regulatory processes in bacterial cells remain unexplored. The current work provides an insight into gene regulation in the antibiotic-resistant nosocomial reference strains treated with iodine-containing nanoparticles. This study sheds light on unexplored bioactivities of iodine and the mechanisms of its antibacterial effect when applied in sublethal concentrations. This knowledge will aid in the future design of new drugs against antibiotic-resistant infections.Ilya S. KorotetskiySergey V. ShilovTatyana V. KuznetsovaAleksandr I. IlinMonique JoubertSetshaba TaukobongOleg N. RevaAmerican Society for MicrobiologyarticletranscriptomicsEscherichia coliStaphylococcus aureusAcinetobacter baumanniiantibiotic resistanceiodineMicrobiologyQR1-502ENmSystems, Vol 6, Iss 2 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	transcriptomics Escherichia coli Staphylococcus aureus Acinetobacter baumannii antibiotic resistance iodine Microbiology QR1-502
spellingShingle	transcriptomics Escherichia coli Staphylococcus aureus Acinetobacter baumannii antibiotic resistance iodine Microbiology QR1-502 Ilya S. Korotetskiy Sergey V. Shilov Tatyana V. Kuznetsova Aleksandr I. Ilin Monique Joubert Setshaba Taukobong Oleg N. Reva Comparison of Transcriptional Responses and Metabolic Alterations in Three Multidrug-Resistant Model Microorganisms, <named-content content-type="genus-species">Staphylococcus aureus</named-content> ATCC BAA-39, <named-content content-type="genus-species">Escherichia coli</named-content> ATCC BAA-196, and <named-content content-type="genus-species">Acinetobacter baumannii</named-content> ATCC BAA-1790, on Exposure to Iodine-Containing Nano-micelle Drug FS-1
description	ABSTRACT Iodine is one of the oldest antimicrobial agents. Until now, there have been no reports on acquiring resistance to iodine. Recent studies showed promising results on application of iodine-containing nano-micelles, FS-1, against antibiotic-resistant pathogens as a supplement to antibiotic therapy. The mechanisms of the action, however, remain unclear. The aim of this study was to perform a holistic analysis and comparison of gene regulation in three phylogenetically distant multidrug-resistant reference strains representing pathogens associated with nosocomial infections from the ATCC culture collection: Escherichia coli BAA-196, Staphylococcus aureus BAA-39, and Acinetobacter baumannii BAA-1790. These cultures were treated by a 5-min exposure to sublethal concentrations of the iodine-containing drug FS-1 applied in the late lagging phase and the middle of the logarithmic growth phase. Complete genome sequences of these strains were obtained in the previous studies. Gene regulation was studied by total RNA extraction and Ion Torrent sequencing followed by mapping the RNA reads against the reference genome sequences and statistical processing of read counts using the DESeq2 algorithm. It was found that the treatment of bacteria with FS-1 profoundly affected the expression of many genes involved in the central metabolic pathways; however, alterations of the gene expression profiles were species specific and depended on the growth phase. Disruption of respiratory electron transfer membrane complexes, increased penetrability of bacterial cell walls, and osmotic and oxidative stresses leading to DNA damage were the major factors influencing the treated bacteria. IMPORTANCE Infections caused by antibiotic-resistant bacteria threaten public health worldwide. Combinatorial therapy in which antibiotics are administered together with supplementary drugs improving susceptibility of pathogens to the regular antibiotics is considered a promising way to overcome this problem. An induction of antibiotic resistance reversion by the iodine-containing nano-micelle drug FS-1 has been reported recently. This drug is currently under clinical trials in Kazakhstan against multidrug-resistant tuberculosis. The effects of released iodine on metabolic and regulatory processes in bacterial cells remain unexplored. The current work provides an insight into gene regulation in the antibiotic-resistant nosocomial reference strains treated with iodine-containing nanoparticles. This study sheds light on unexplored bioactivities of iodine and the mechanisms of its antibacterial effect when applied in sublethal concentrations. This knowledge will aid in the future design of new drugs against antibiotic-resistant infections.
format	article
author	Ilya S. Korotetskiy Sergey V. Shilov Tatyana V. Kuznetsova Aleksandr I. Ilin Monique Joubert Setshaba Taukobong Oleg N. Reva
author_facet	Ilya S. Korotetskiy Sergey V. Shilov Tatyana V. Kuznetsova Aleksandr I. Ilin Monique Joubert Setshaba Taukobong Oleg N. Reva
author_sort	Ilya S. Korotetskiy
title	Comparison of Transcriptional Responses and Metabolic Alterations in Three Multidrug-Resistant Model Microorganisms, <named-content content-type="genus-species">Staphylococcus aureus</named-content> ATCC BAA-39, <named-content content-type="genus-species">Escherichia coli</named-content> ATCC BAA-196, and <named-content content-type="genus-species">Acinetobacter baumannii</named-content> ATCC BAA-1790, on Exposure to Iodine-Containing Nano-micelle Drug FS-1
title_short	Comparison of Transcriptional Responses and Metabolic Alterations in Three Multidrug-Resistant Model Microorganisms, <named-content content-type="genus-species">Staphylococcus aureus</named-content> ATCC BAA-39, <named-content content-type="genus-species">Escherichia coli</named-content> ATCC BAA-196, and <named-content content-type="genus-species">Acinetobacter baumannii</named-content> ATCC BAA-1790, on Exposure to Iodine-Containing Nano-micelle Drug FS-1
title_full	Comparison of Transcriptional Responses and Metabolic Alterations in Three Multidrug-Resistant Model Microorganisms, <named-content content-type="genus-species">Staphylococcus aureus</named-content> ATCC BAA-39, <named-content content-type="genus-species">Escherichia coli</named-content> ATCC BAA-196, and <named-content content-type="genus-species">Acinetobacter baumannii</named-content> ATCC BAA-1790, on Exposure to Iodine-Containing Nano-micelle Drug FS-1
title_fullStr	Comparison of Transcriptional Responses and Metabolic Alterations in Three Multidrug-Resistant Model Microorganisms, <named-content content-type="genus-species">Staphylococcus aureus</named-content> ATCC BAA-39, <named-content content-type="genus-species">Escherichia coli</named-content> ATCC BAA-196, and <named-content content-type="genus-species">Acinetobacter baumannii</named-content> ATCC BAA-1790, on Exposure to Iodine-Containing Nano-micelle Drug FS-1
title_full_unstemmed	Comparison of Transcriptional Responses and Metabolic Alterations in Three Multidrug-Resistant Model Microorganisms, <named-content content-type="genus-species">Staphylococcus aureus</named-content> ATCC BAA-39, <named-content content-type="genus-species">Escherichia coli</named-content> ATCC BAA-196, and <named-content content-type="genus-species">Acinetobacter baumannii</named-content> ATCC BAA-1790, on Exposure to Iodine-Containing Nano-micelle Drug FS-1
title_sort	comparison of transcriptional responses and metabolic alterations in three multidrug-resistant model microorganisms, <named-content content-type="genus-species">staphylococcus aureus</named-content> atcc baa-39, <named-content content-type="genus-species">escherichia coli</named-content> atcc baa-196, and <named-content content-type="genus-species">acinetobacter baumannii</named-content> atcc baa-1790, on exposure to iodine-containing nano-micelle drug fs-1
publisher	American Society for Microbiology
publishDate	2021
url	https://doaj.org/article/dbd82ec33fd84895be58be61dad53bdc
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