Serial protein crystallography in an electron microscope

For conventional three-dimensional microcrystal electron diffraction (3D ED/MicroED), a crystal is slowly rotated under an electron beam, leading to inevitable accumulation of radiation damage during data collection. In this work, the authors present a serial electron diffraction method, where still...

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Autores principales:	Robert Bücker, Pascal Hogan-Lamarre, Pedram Mehrabi, Eike C. Schulz, Lindsey A. Bultema, Yaroslav Gevorkov, Wolfgang Brehm, Oleksandr Yefanov, Dominik Oberthür, Günther H. Kassier, R. J. Dwayne Miller
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/dbd92ddaa279416f9b79929ff4e04966
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spelling	oai:doaj.org-article:dbd92ddaa279416f9b79929ff4e049662021-12-02T16:56:51ZSerial protein crystallography in an electron microscope10.1038/s41467-020-14793-02041-1723https://doaj.org/article/dbd92ddaa279416f9b79929ff4e049662020-02-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-14793-0https://doaj.org/toc/2041-1723For conventional three-dimensional microcrystal electron diffraction (3D ED/MicroED), a crystal is slowly rotated under an electron beam, leading to inevitable accumulation of radiation damage during data collection. In this work, the authors present a serial electron diffraction method, where still diffraction patterns from many protein nanocrystals are rapidly recorded and merged, which minimises radiation damage and only requires a slightly modified standard scanning transmission electron microscope.Robert BückerPascal Hogan-LamarrePedram MehrabiEike C. SchulzLindsey A. BultemaYaroslav GevorkovWolfgang BrehmOleksandr YefanovDominik OberthürGünther H. KassierR. J. Dwayne MillerNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-8 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Robert Bücker Pascal Hogan-Lamarre Pedram Mehrabi Eike C. Schulz Lindsey A. Bultema Yaroslav Gevorkov Wolfgang Brehm Oleksandr Yefanov Dominik Oberthür Günther H. Kassier R. J. Dwayne Miller Serial protein crystallography in an electron microscope
description	For conventional three-dimensional microcrystal electron diffraction (3D ED/MicroED), a crystal is slowly rotated under an electron beam, leading to inevitable accumulation of radiation damage during data collection. In this work, the authors present a serial electron diffraction method, where still diffraction patterns from many protein nanocrystals are rapidly recorded and merged, which minimises radiation damage and only requires a slightly modified standard scanning transmission electron microscope.
format	article
author	Robert Bücker Pascal Hogan-Lamarre Pedram Mehrabi Eike C. Schulz Lindsey A. Bultema Yaroslav Gevorkov Wolfgang Brehm Oleksandr Yefanov Dominik Oberthür Günther H. Kassier R. J. Dwayne Miller
author_facet	Robert Bücker Pascal Hogan-Lamarre Pedram Mehrabi Eike C. Schulz Lindsey A. Bultema Yaroslav Gevorkov Wolfgang Brehm Oleksandr Yefanov Dominik Oberthür Günther H. Kassier R. J. Dwayne Miller
author_sort	Robert Bücker
title	Serial protein crystallography in an electron microscope
title_short	Serial protein crystallography in an electron microscope
title_full	Serial protein crystallography in an electron microscope
title_fullStr	Serial protein crystallography in an electron microscope
title_full_unstemmed	Serial protein crystallography in an electron microscope
title_sort	serial protein crystallography in an electron microscope
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/dbd92ddaa279416f9b79929ff4e04966
work_keys_str_mv	AT robertbucker serialproteincrystallographyinanelectronmicroscope AT pascalhoganlamarre serialproteincrystallographyinanelectronmicroscope AT pedrammehrabi serialproteincrystallographyinanelectronmicroscope AT eikecschulz serialproteincrystallographyinanelectronmicroscope AT lindseyabultema serialproteincrystallographyinanelectronmicroscope AT yaroslavgevorkov serialproteincrystallographyinanelectronmicroscope AT wolfgangbrehm serialproteincrystallographyinanelectronmicroscope AT oleksandryefanov serialproteincrystallographyinanelectronmicroscope AT dominikoberthur serialproteincrystallographyinanelectronmicroscope AT guntherhkassier serialproteincrystallographyinanelectronmicroscope AT rjdwaynemiller serialproteincrystallographyinanelectronmicroscope
_version_	1718382710015131648

Serial protein crystallography in an electron microscope

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