Human fucci pancreatic Beta cell lines: new tools to study Beta cell cycle and terminal differentiation.

Regulation of cell cycle in beta cells is poorly understood, especially in humans. We exploited here the recently described human pancreatic beta cell line EndoC-βH2 to set up experimental systems for cell cycle studies. We derived 2 populations from EndoC-βH2 cells that stably harbor the 2 genes en...

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Autores principales:	Géraldine Carlier, Alicia Maugein, Corinne Cordier, Séverine Pechberty, Meriem Garfa-Traoré, Patrick Martin, Raphaël Scharfmann, Olivier Albagli
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/dbda6d14d8c4423f859fef3854c9af79
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spelling	oai:doaj.org-article:dbda6d14d8c4423f859fef3854c9af792021-11-25T05:59:12ZHuman fucci pancreatic Beta cell lines: new tools to study Beta cell cycle and terminal differentiation.1932-620310.1371/journal.pone.0108202https://doaj.org/article/dbda6d14d8c4423f859fef3854c9af792014-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0108202https://doaj.org/toc/1932-6203Regulation of cell cycle in beta cells is poorly understood, especially in humans. We exploited here the recently described human pancreatic beta cell line EndoC-βH2 to set up experimental systems for cell cycle studies. We derived 2 populations from EndoC-βH2 cells that stably harbor the 2 genes encoding the Fucci fluorescent indicators of cell cycle, either from two vectors, or from a unique bicistronic vector. In proliferating non-synchronized cells, the 2 Fucci indicators revealed cells in the expected phases of cell cycle, with orange and green cells being in G1 and S/G2/M cells, respectively, and allowed the sorting of cells in different substeps of G1. The Fucci indicators also faithfully red out alterations in human beta cell proliferative activity since a mitogen-rich medium decreased the proportion of orange cells and inflated the green population, while reciprocal changes were observed when cells were induced to cease proliferation and increased expression of some beta cell genes. In the last situation, acquisition of a more differentiated beta cell phenotype correlates with an increased intensity in orange fluorescence. Hence Fucci beta cell lines provide new tools to address important questions regarding human beta cell cycle and differentiation.Géraldine CarlierAlicia MaugeinCorinne CordierSéverine PechbertyMeriem Garfa-TraoréPatrick MartinRaphaël ScharfmannOlivier AlbagliPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 9, p e108202 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Géraldine Carlier Alicia Maugein Corinne Cordier Séverine Pechberty Meriem Garfa-Traoré Patrick Martin Raphaël Scharfmann Olivier Albagli Human fucci pancreatic Beta cell lines: new tools to study Beta cell cycle and terminal differentiation.
description	Regulation of cell cycle in beta cells is poorly understood, especially in humans. We exploited here the recently described human pancreatic beta cell line EndoC-βH2 to set up experimental systems for cell cycle studies. We derived 2 populations from EndoC-βH2 cells that stably harbor the 2 genes encoding the Fucci fluorescent indicators of cell cycle, either from two vectors, or from a unique bicistronic vector. In proliferating non-synchronized cells, the 2 Fucci indicators revealed cells in the expected phases of cell cycle, with orange and green cells being in G1 and S/G2/M cells, respectively, and allowed the sorting of cells in different substeps of G1. The Fucci indicators also faithfully red out alterations in human beta cell proliferative activity since a mitogen-rich medium decreased the proportion of orange cells and inflated the green population, while reciprocal changes were observed when cells were induced to cease proliferation and increased expression of some beta cell genes. In the last situation, acquisition of a more differentiated beta cell phenotype correlates with an increased intensity in orange fluorescence. Hence Fucci beta cell lines provide new tools to address important questions regarding human beta cell cycle and differentiation.
format	article
author	Géraldine Carlier Alicia Maugein Corinne Cordier Séverine Pechberty Meriem Garfa-Traoré Patrick Martin Raphaël Scharfmann Olivier Albagli
author_facet	Géraldine Carlier Alicia Maugein Corinne Cordier Séverine Pechberty Meriem Garfa-Traoré Patrick Martin Raphaël Scharfmann Olivier Albagli
author_sort	Géraldine Carlier
title	Human fucci pancreatic Beta cell lines: new tools to study Beta cell cycle and terminal differentiation.
title_short	Human fucci pancreatic Beta cell lines: new tools to study Beta cell cycle and terminal differentiation.
title_full	Human fucci pancreatic Beta cell lines: new tools to study Beta cell cycle and terminal differentiation.
title_fullStr	Human fucci pancreatic Beta cell lines: new tools to study Beta cell cycle and terminal differentiation.
title_full_unstemmed	Human fucci pancreatic Beta cell lines: new tools to study Beta cell cycle and terminal differentiation.
title_sort	human fucci pancreatic beta cell lines: new tools to study beta cell cycle and terminal differentiation.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/dbda6d14d8c4423f859fef3854c9af79
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_version_	1718414310543196160

Human fucci pancreatic Beta cell lines: new tools to study Beta cell cycle and terminal differentiation.

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