Optical projection tomography as a quantitative tool for analysis of cell morphology and density in 3D hydrogels

Abstract Assessing cell morphology and function, as well as biomaterial performance in cell cultures, is one of the key challenges in cell biology and tissue engineering (TE) research. In TE, there is an urgent need for methods to image actual three-dimensional (3D) cell cultures and access the livi...

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Autores principales:	Birhanu Belay, Janne T. Koivisto, Jenny Parraga, Olli Koskela, Toni Montonen, Minna Kellomäki, Edite Figueiras, Jari Hyttinen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/dbe3fefd5abc47bd82a0b4c59227cbf0
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spelling	oai:doaj.org-article:dbe3fefd5abc47bd82a0b4c59227cbf02021-12-02T17:04:07ZOptical projection tomography as a quantitative tool for analysis of cell morphology and density in 3D hydrogels10.1038/s41598-021-85996-82045-2322https://doaj.org/article/dbe3fefd5abc47bd82a0b4c59227cbf02021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85996-8https://doaj.org/toc/2045-2322Abstract Assessing cell morphology and function, as well as biomaterial performance in cell cultures, is one of the key challenges in cell biology and tissue engineering (TE) research. In TE, there is an urgent need for methods to image actual three-dimensional (3D) cell cultures and access the living cells. This is difficult using established optical microscopy techniques such as wide-field or confocal microscopy. To address the problem, we have developed a new protocol using Optical Projection Tomography (OPT) to extract quantitative and qualitative measurements from hydrogel cell cultures. Using our tools, we demonstrated the method by analyzing cell response in three different hydrogel formulations in 3D with 1.5 mm diameter samples of: gellan gum (GG), gelatin functionalized gellan gum (gelatin-GG), and Geltrex. We investigated cell morphology, density, distribution, and viability in 3D living cells. Our results showed the usability of the method to quantify the cellular responses to biomaterial environment. We observed that an elongated morphology of cells, thus good material response, in gelatin-GG and Geltrex hydrogels compared with basic GG. Our results show that OPT has a sensitivity to assess in real 3D cultures the differences of cellular responses to the properties of biomaterials supporting the cells.Birhanu BelayJanne T. KoivistoJenny ParragaOlli KoskelaToni MontonenMinna KellomäkiEdite FigueirasJari HyttinenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Birhanu Belay Janne T. Koivisto Jenny Parraga Olli Koskela Toni Montonen Minna Kellomäki Edite Figueiras Jari Hyttinen Optical projection tomography as a quantitative tool for analysis of cell morphology and density in 3D hydrogels
description	Abstract Assessing cell morphology and function, as well as biomaterial performance in cell cultures, is one of the key challenges in cell biology and tissue engineering (TE) research. In TE, there is an urgent need for methods to image actual three-dimensional (3D) cell cultures and access the living cells. This is difficult using established optical microscopy techniques such as wide-field or confocal microscopy. To address the problem, we have developed a new protocol using Optical Projection Tomography (OPT) to extract quantitative and qualitative measurements from hydrogel cell cultures. Using our tools, we demonstrated the method by analyzing cell response in three different hydrogel formulations in 3D with 1.5 mm diameter samples of: gellan gum (GG), gelatin functionalized gellan gum (gelatin-GG), and Geltrex. We investigated cell morphology, density, distribution, and viability in 3D living cells. Our results showed the usability of the method to quantify the cellular responses to biomaterial environment. We observed that an elongated morphology of cells, thus good material response, in gelatin-GG and Geltrex hydrogels compared with basic GG. Our results show that OPT has a sensitivity to assess in real 3D cultures the differences of cellular responses to the properties of biomaterials supporting the cells.
format	article
author	Birhanu Belay Janne T. Koivisto Jenny Parraga Olli Koskela Toni Montonen Minna Kellomäki Edite Figueiras Jari Hyttinen
author_facet	Birhanu Belay Janne T. Koivisto Jenny Parraga Olli Koskela Toni Montonen Minna Kellomäki Edite Figueiras Jari Hyttinen
author_sort	Birhanu Belay
title	Optical projection tomography as a quantitative tool for analysis of cell morphology and density in 3D hydrogels
title_short	Optical projection tomography as a quantitative tool for analysis of cell morphology and density in 3D hydrogels
title_full	Optical projection tomography as a quantitative tool for analysis of cell morphology and density in 3D hydrogels
title_fullStr	Optical projection tomography as a quantitative tool for analysis of cell morphology and density in 3D hydrogels
title_full_unstemmed	Optical projection tomography as a quantitative tool for analysis of cell morphology and density in 3D hydrogels
title_sort	optical projection tomography as a quantitative tool for analysis of cell morphology and density in 3d hydrogels
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/dbe3fefd5abc47bd82a0b4c59227cbf0
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Optical projection tomography as a quantitative tool for analysis of cell morphology and density in 3D hydrogels

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