Liver microRNA hsa-miR-125a-5p in HBV chronic infection: correlation with HBV replication and disease progression.
To study in HBsAg chronic carriers the expression of liver hsa-miR-125a-5p and its correlation with liver HBV-DNA values and clinical presentation, 27 consecutive Caucasian, HBsAg/anti-HBe/HBV-DNA-positive patients who were naive to nucleos(t)ide analogues and interferon therapy and had no marker of...
Guardado en:
| Autores principales: | , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Public Library of Science (PLoS)
2013
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/dbe42f1f94aa4e6b8d74ddca396cca01 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:dbe42f1f94aa4e6b8d74ddca396cca01 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:dbe42f1f94aa4e6b8d74ddca396cca012021-11-18T07:38:54ZLiver microRNA hsa-miR-125a-5p in HBV chronic infection: correlation with HBV replication and disease progression.1932-620310.1371/journal.pone.0065336https://doaj.org/article/dbe42f1f94aa4e6b8d74ddca396cca012013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23843939/?tool=EBIhttps://doaj.org/toc/1932-6203To study in HBsAg chronic carriers the expression of liver hsa-miR-125a-5p and its correlation with liver HBV-DNA values and clinical presentation, 27 consecutive Caucasian, HBsAg/anti-HBe/HBV-DNA-positive patients who were naive to nucleos(t)ide analogues and interferon therapy and had no marker of HCV, HDV or HIV infection and no history of alcohol intake were enrolled. For each patient, liver HBV DNA and liver hsa-miR-125a-5p were quantified by real-time PCR in relation to β-globin DNA or RNU6B, respectively. Liver fibrosis and necroinflammation were graded by applying Ishak's scoring system. Liver hsa-miR-125a-5p was detected in all patients enrolled and a correlation between its concentration and liver HBV DNA was demonstrated (p<0.0001). Higher liver hsa-miR-125a-5p concentrations were observed in patients with HBV-DNA plasma level >10(3) IU/ml (p<0.02), in those with HAI >6 (p = 0.02) and those with fibrosis score >2 (p<0.02) than in patients with lower scores. Higher HBV-DNA liver concentrations were found in patients with abnormal AST (p = 0.005) and ALT serum levels (p = 0.05), in those with serum HBV DNA higher than 10E3 IU/mL (p = 0.001) and those with fibrosis score >2 (p = 0.02) than in patients with a lower load. By multivariate logistic regression analysis, liver hsa-miR-125a-5p was identified as an independent predictor of disease progression: O.R. = 4.21, C.I. 95% = 1.08-16.43, p<0.05, for HAI >6; O.R. = 3.12, C.I. 95% = 1.17-8.27, p<0.05, for fibrosis score >2. In conclusion, in HBsAg/anti-HBe-positive patients, the liver hsa-miR-125a-5p level correlated with liver and plasma HBV-DNA values and was associated to a more severe disease progression.Nicola CoppolaNicoletta PotenzaMariantonietta PisaturoNicola MoscaGilda TonzielloGiuseppe SignorielloVincenzo MessinaCaterina SagnelliAniello RussoEvangelista SagnelliPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 7, p e65336 (2013) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Nicola Coppola Nicoletta Potenza Mariantonietta Pisaturo Nicola Mosca Gilda Tonziello Giuseppe Signoriello Vincenzo Messina Caterina Sagnelli Aniello Russo Evangelista Sagnelli Liver microRNA hsa-miR-125a-5p in HBV chronic infection: correlation with HBV replication and disease progression. |
| description |
To study in HBsAg chronic carriers the expression of liver hsa-miR-125a-5p and its correlation with liver HBV-DNA values and clinical presentation, 27 consecutive Caucasian, HBsAg/anti-HBe/HBV-DNA-positive patients who were naive to nucleos(t)ide analogues and interferon therapy and had no marker of HCV, HDV or HIV infection and no history of alcohol intake were enrolled. For each patient, liver HBV DNA and liver hsa-miR-125a-5p were quantified by real-time PCR in relation to β-globin DNA or RNU6B, respectively. Liver fibrosis and necroinflammation were graded by applying Ishak's scoring system. Liver hsa-miR-125a-5p was detected in all patients enrolled and a correlation between its concentration and liver HBV DNA was demonstrated (p<0.0001). Higher liver hsa-miR-125a-5p concentrations were observed in patients with HBV-DNA plasma level >10(3) IU/ml (p<0.02), in those with HAI >6 (p = 0.02) and those with fibrosis score >2 (p<0.02) than in patients with lower scores. Higher HBV-DNA liver concentrations were found in patients with abnormal AST (p = 0.005) and ALT serum levels (p = 0.05), in those with serum HBV DNA higher than 10E3 IU/mL (p = 0.001) and those with fibrosis score >2 (p = 0.02) than in patients with a lower load. By multivariate logistic regression analysis, liver hsa-miR-125a-5p was identified as an independent predictor of disease progression: O.R. = 4.21, C.I. 95% = 1.08-16.43, p<0.05, for HAI >6; O.R. = 3.12, C.I. 95% = 1.17-8.27, p<0.05, for fibrosis score >2. In conclusion, in HBsAg/anti-HBe-positive patients, the liver hsa-miR-125a-5p level correlated with liver and plasma HBV-DNA values and was associated to a more severe disease progression. |
| format |
article |
| author |
Nicola Coppola Nicoletta Potenza Mariantonietta Pisaturo Nicola Mosca Gilda Tonziello Giuseppe Signoriello Vincenzo Messina Caterina Sagnelli Aniello Russo Evangelista Sagnelli |
| author_facet |
Nicola Coppola Nicoletta Potenza Mariantonietta Pisaturo Nicola Mosca Gilda Tonziello Giuseppe Signoriello Vincenzo Messina Caterina Sagnelli Aniello Russo Evangelista Sagnelli |
| author_sort |
Nicola Coppola |
| title |
Liver microRNA hsa-miR-125a-5p in HBV chronic infection: correlation with HBV replication and disease progression. |
| title_short |
Liver microRNA hsa-miR-125a-5p in HBV chronic infection: correlation with HBV replication and disease progression. |
| title_full |
Liver microRNA hsa-miR-125a-5p in HBV chronic infection: correlation with HBV replication and disease progression. |
| title_fullStr |
Liver microRNA hsa-miR-125a-5p in HBV chronic infection: correlation with HBV replication and disease progression. |
| title_full_unstemmed |
Liver microRNA hsa-miR-125a-5p in HBV chronic infection: correlation with HBV replication and disease progression. |
| title_sort |
liver microrna hsa-mir-125a-5p in hbv chronic infection: correlation with hbv replication and disease progression. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2013 |
| url |
https://doaj.org/article/dbe42f1f94aa4e6b8d74ddca396cca01 |
| work_keys_str_mv |
AT nicolacoppola livermicrornahsamir125a5pinhbvchronicinfectioncorrelationwithhbvreplicationanddiseaseprogression AT nicolettapotenza livermicrornahsamir125a5pinhbvchronicinfectioncorrelationwithhbvreplicationanddiseaseprogression AT mariantoniettapisaturo livermicrornahsamir125a5pinhbvchronicinfectioncorrelationwithhbvreplicationanddiseaseprogression AT nicolamosca livermicrornahsamir125a5pinhbvchronicinfectioncorrelationwithhbvreplicationanddiseaseprogression AT gildatonziello livermicrornahsamir125a5pinhbvchronicinfectioncorrelationwithhbvreplicationanddiseaseprogression AT giuseppesignoriello livermicrornahsamir125a5pinhbvchronicinfectioncorrelationwithhbvreplicationanddiseaseprogression AT vincenzomessina livermicrornahsamir125a5pinhbvchronicinfectioncorrelationwithhbvreplicationanddiseaseprogression AT caterinasagnelli livermicrornahsamir125a5pinhbvchronicinfectioncorrelationwithhbvreplicationanddiseaseprogression AT aniellorusso livermicrornahsamir125a5pinhbvchronicinfectioncorrelationwithhbvreplicationanddiseaseprogression AT evangelistasagnelli livermicrornahsamir125a5pinhbvchronicinfectioncorrelationwithhbvreplicationanddiseaseprogression |
| _version_ |
1718423193236013056 |