In vitro antibacterial activities of crude extracts of nine plants on multidrug resistance bacterial isolates of wound infections

Abstract. Prastiyanto ME, Dewi NMBA, Pratiningtias TD, Pratiwi NMR, Windayani A, Wahyunengsih E, Astuti, Amir E, Wardoyo FA. 2021. In vitro antibacterial activities of crude extracts of nine plants on multidrug resistance bacterial isolates of wound infections. Biodiversitas 22: 2641-2647. Wound inf...

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Autores principales:	Muhammad Evy Prastiyanto, NI MADE BUNGA ANGGELIA DEWI, TUSY DIAH PRATININGTIAS, NI MADE RAI PRATIWI, ANGGIS WINDAYANI, EKA WAHYUNENGSIH, ASTUTI ASTUTI, ELVIRA AMIR, FANDHI ADI WARDOYO
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2021
Materias:	in vitro antibacterial activities; wound infection; mrsa, esβl-producing escherichia coli, crpa; bitter melon Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/dbe44a3eba4440708274b84b8800324e
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spelling	oai:doaj.org-article:dbe44a3eba4440708274b84b8800324e2021-11-22T12:16:56ZIn vitro antibacterial activities of crude extracts of nine plants on multidrug resistance bacterial isolates of wound infections1412-033X2085-472210.13057/biodiv/d220712https://doaj.org/article/dbe44a3eba4440708274b84b8800324e2021-06-01T00:00:00Zhttps://smujo.id/biodiv/article/view/8468https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Prastiyanto ME, Dewi NMBA, Pratiningtias TD, Pratiwi NMR, Windayani A, Wahyunengsih E, Astuti, Amir E, Wardoyo FA. 2021. In vitro antibacterial activities of crude extracts of nine plants on multidrug resistance bacterial isolates of wound infections. Biodiversitas 22: 2641-2647. Wound infections caused by bacteria is a become serious health problems, multidrug resistance bacteria (MDR) have increased this problem more severely, and therefore, antibacterial agents from natural biological sources are necessary to overcome these problems. This study examined the antibacterial activities of nine plants, i.e. garlic (Allium sativum), Solo garlic (Allium sativum), Java plum leaf (Syzygium cumini), Java plum fruit (Syzygium cumini), lime (Citrus aurantifolia), Kaffir lime (Citrus hystrix), Siamese weed (Chromolaena odorata), mangosteen (Garcinia mangostana) and bitter melon (Momordica charantia), against MDR bacteria isolated from wounds. The antibacterial activities were evaluated using agar well diffusion assay to determine the inhibition zones, and microdilution method to determine the value of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). The best antibacterial activities were calculated as the most extensive inhibition zones with the smallest MIC and MBC values. Ethanol extracts from five plants (garlic, Solo garlic, Java plum (leaf), Kaffir lime and bitter melon) showed antibacterial activities against three MDR bacteria isolated from wounds. The bitter melon extract had the largest zones, 19.3 mm (methicillin-resistant Staphylococcus aureus [MRSA]), 10.6 mm (ESBL-producing Escherichia coli), and 13 mm (carbapenemase-resistant Pseudomonas aeruginosa [CRPA]) with the smallest MIC and MBC values against MRSA (3.12 and 25 mg/mL), ESBL- producing E. coli (12.25 and 50 mg/mL), and CRPA (6.25 and 25 mg/mL). This concludes that bitter melon has the potential to be developed as an antibacterial agent, particularly against MRSA strains, ESBL-producing E. coli, and CRPA that cause wound infections. Further in vivo research and the discovery of modes of action are needed to explain the antibacterial effects.Muhammad Evy PrastiyantoNI MADE BUNGA ANGGELIA DEWITUSY DIAH PRATININGTIASNI MADE RAI PRATIWIANGGIS WINDAYANIEKA WAHYUNENGSIHASTUTI ASTUTIELVIRA AMIRFANDHI ADI WARDOYOMBI & UNS Soloarticlein vitro antibacterial activities; wound infection; mrsa, esβl-producing escherichia coli, crpa; bitter melonBiology (General)QH301-705.5ENBiodiversitas, Vol 22, Iss 7 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	in vitro antibacterial activities; wound infection; mrsa, esβl-producing escherichia coli, crpa; bitter melon Biology (General) QH301-705.5
spellingShingle	in vitro antibacterial activities; wound infection; mrsa, esβl-producing escherichia coli, crpa; bitter melon Biology (General) QH301-705.5 Muhammad Evy Prastiyanto NI MADE BUNGA ANGGELIA DEWI TUSY DIAH PRATININGTIAS NI MADE RAI PRATIWI ANGGIS WINDAYANI EKA WAHYUNENGSIH ASTUTI ASTUTI ELVIRA AMIR FANDHI ADI WARDOYO In vitro antibacterial activities of crude extracts of nine plants on multidrug resistance bacterial isolates of wound infections
description	Abstract. Prastiyanto ME, Dewi NMBA, Pratiningtias TD, Pratiwi NMR, Windayani A, Wahyunengsih E, Astuti, Amir E, Wardoyo FA. 2021. In vitro antibacterial activities of crude extracts of nine plants on multidrug resistance bacterial isolates of wound infections. Biodiversitas 22: 2641-2647. Wound infections caused by bacteria is a become serious health problems, multidrug resistance bacteria (MDR) have increased this problem more severely, and therefore, antibacterial agents from natural biological sources are necessary to overcome these problems. This study examined the antibacterial activities of nine plants, i.e. garlic (Allium sativum), Solo garlic (Allium sativum), Java plum leaf (Syzygium cumini), Java plum fruit (Syzygium cumini), lime (Citrus aurantifolia), Kaffir lime (Citrus hystrix), Siamese weed (Chromolaena odorata), mangosteen (Garcinia mangostana) and bitter melon (Momordica charantia), against MDR bacteria isolated from wounds. The antibacterial activities were evaluated using agar well diffusion assay to determine the inhibition zones, and microdilution method to determine the value of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). The best antibacterial activities were calculated as the most extensive inhibition zones with the smallest MIC and MBC values. Ethanol extracts from five plants (garlic, Solo garlic, Java plum (leaf), Kaffir lime and bitter melon) showed antibacterial activities against three MDR bacteria isolated from wounds. The bitter melon extract had the largest zones, 19.3 mm (methicillin-resistant Staphylococcus aureus [MRSA]), 10.6 mm (ESBL-producing Escherichia coli), and 13 mm (carbapenemase-resistant Pseudomonas aeruginosa [CRPA]) with the smallest MIC and MBC values against MRSA (3.12 and 25 mg/mL), ESBL- producing E. coli (12.25 and 50 mg/mL), and CRPA (6.25 and 25 mg/mL). This concludes that bitter melon has the potential to be developed as an antibacterial agent, particularly against MRSA strains, ESBL-producing E. coli, and CRPA that cause wound infections. Further in vivo research and the discovery of modes of action are needed to explain the antibacterial effects.
format	article
author	Muhammad Evy Prastiyanto NI MADE BUNGA ANGGELIA DEWI TUSY DIAH PRATININGTIAS NI MADE RAI PRATIWI ANGGIS WINDAYANI EKA WAHYUNENGSIH ASTUTI ASTUTI ELVIRA AMIR FANDHI ADI WARDOYO
author_facet	Muhammad Evy Prastiyanto NI MADE BUNGA ANGGELIA DEWI TUSY DIAH PRATININGTIAS NI MADE RAI PRATIWI ANGGIS WINDAYANI EKA WAHYUNENGSIH ASTUTI ASTUTI ELVIRA AMIR FANDHI ADI WARDOYO
author_sort	Muhammad Evy Prastiyanto
title	In vitro antibacterial activities of crude extracts of nine plants on multidrug resistance bacterial isolates of wound infections
title_short	In vitro antibacterial activities of crude extracts of nine plants on multidrug resistance bacterial isolates of wound infections
title_full	In vitro antibacterial activities of crude extracts of nine plants on multidrug resistance bacterial isolates of wound infections
title_fullStr	In vitro antibacterial activities of crude extracts of nine plants on multidrug resistance bacterial isolates of wound infections
title_full_unstemmed	In vitro antibacterial activities of crude extracts of nine plants on multidrug resistance bacterial isolates of wound infections
title_sort	in vitro antibacterial activities of crude extracts of nine plants on multidrug resistance bacterial isolates of wound infections
publisher	MBI & UNS Solo
publishDate	2021
url	https://doaj.org/article/dbe44a3eba4440708274b84b8800324e
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In vitro antibacterial activities of crude extracts of nine plants on multidrug resistance bacterial isolates of wound infections

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