CUL4A promotes cell invasion in gastric cancer by activating the NF-κB signaling pathway
Yu Gong,* Xiao-Jun Xiang,* Miao Feng, Jun Chen, Zi-Ling Fang, Jian-Ping Xiong, Department of Oncology, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, People’s Republic of China *These authors contributed equally to this work Abstract: Cullin 4A (CUL4A) overexpress...
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Dove Medical Press
2017
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oai:doaj.org-article:dbe4f2eb1a854f6b814badc7f7b54dc42021-12-02T01:36:15ZCUL4A promotes cell invasion in gastric cancer by activating the NF-κB signaling pathway1177-5491https://doaj.org/article/dbe4f2eb1a854f6b814badc7f7b54dc42017-04-01T00:00:00Zhttps://www.dovepress.com/cul4a-promotes-cell-invasion-in-gastric-cancer-by-activating-the-nf-ka-peer-reviewed-article-BTThttps://doaj.org/toc/1177-5491Yu Gong,* Xiao-Jun Xiang,* Miao Feng, Jun Chen, Zi-Ling Fang, Jian-Ping Xiong, Department of Oncology, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, People’s Republic of China *These authors contributed equally to this work Abstract: Cullin 4A (CUL4A) overexpression has been reported to be involved in the carcinogenesis and progression of many malignant tumors. However, the role of CUL4A in the progression of gastric cancer (GC) remains unclear. In this study, we explored whether and how CUL4A regulates proinflammatory signaling to promote GC cell invasion. Our results showed that knockdown of CUL4A inhibited GC cell migration and invasion induced by lipopolysaccharide (LPS) stimulation. We also found that both CUL4A and nuclear factor-kappa B (NF-κB) protein expressions were enhanced by LPS stimulation in HGC27 GC cell lines. Furthermore, knockdown of CUL4A decreased the protein expression of NF-κB and mRNA expression of the downstream genes of the NF-κB pathway, such as matrix metalloproteinase (MMP) 2, MMP9, and interleukin-8. Our immunohistochemistry analysis on 50 GC tissue samples also revealed that CUL4A positively correlated with NF-κB expression. Taken together, our findings suggest that CUL4A may promote GC cell invasion by regulating the NF-κB signaling pathway and could be considered as a potential therapeutic target in patients with GC. Keywords: CUL4A, NF-κB, gastric cancer, invasionGong YXiang XJFeng MChen JFang ZXiong JDove Medical PressarticleCUL4ANF- κBgastric cancerinvasionMedicine (General)R5-920ENBiologics: Targets & Therapy, Vol Volume 11, Pp 45-53 (2017) |
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CUL4A NF- κB gastric cancer invasion Medicine (General) R5-920 |
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CUL4A NF- κB gastric cancer invasion Medicine (General) R5-920 Gong Y Xiang XJ Feng M Chen J Fang Z Xiong J CUL4A promotes cell invasion in gastric cancer by activating the NF-κB signaling pathway |
| description |
Yu Gong,* Xiao-Jun Xiang,* Miao Feng, Jun Chen, Zi-Ling Fang, Jian-Ping Xiong, Department of Oncology, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, People’s Republic of China *These authors contributed equally to this work Abstract: Cullin 4A (CUL4A) overexpression has been reported to be involved in the carcinogenesis and progression of many malignant tumors. However, the role of CUL4A in the progression of gastric cancer (GC) remains unclear. In this study, we explored whether and how CUL4A regulates proinflammatory signaling to promote GC cell invasion. Our results showed that knockdown of CUL4A inhibited GC cell migration and invasion induced by lipopolysaccharide (LPS) stimulation. We also found that both CUL4A and nuclear factor-kappa B (NF-κB) protein expressions were enhanced by LPS stimulation in HGC27 GC cell lines. Furthermore, knockdown of CUL4A decreased the protein expression of NF-κB and mRNA expression of the downstream genes of the NF-κB pathway, such as matrix metalloproteinase (MMP) 2, MMP9, and interleukin-8. Our immunohistochemistry analysis on 50 GC tissue samples also revealed that CUL4A positively correlated with NF-κB expression. Taken together, our findings suggest that CUL4A may promote GC cell invasion by regulating the NF-κB signaling pathway and could be considered as a potential therapeutic target in patients with GC. Keywords: CUL4A, NF-κB, gastric cancer, invasion |
| format |
article |
| author |
Gong Y Xiang XJ Feng M Chen J Fang Z Xiong J |
| author_facet |
Gong Y Xiang XJ Feng M Chen J Fang Z Xiong J |
| author_sort |
Gong Y |
| title |
CUL4A promotes cell invasion in gastric cancer by activating the NF-κB signaling pathway |
| title_short |
CUL4A promotes cell invasion in gastric cancer by activating the NF-κB signaling pathway |
| title_full |
CUL4A promotes cell invasion in gastric cancer by activating the NF-κB signaling pathway |
| title_fullStr |
CUL4A promotes cell invasion in gastric cancer by activating the NF-κB signaling pathway |
| title_full_unstemmed |
CUL4A promotes cell invasion in gastric cancer by activating the NF-κB signaling pathway |
| title_sort |
cul4a promotes cell invasion in gastric cancer by activating the nf-κb signaling pathway |
| publisher |
Dove Medical Press |
| publishDate |
2017 |
| url |
https://doaj.org/article/dbe4f2eb1a854f6b814badc7f7b54dc4 |
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