Synthesis and extracellular accumulation of silver nanoparticles by employing radiation-resistant Deinococcus radiodurans, their characterization, and determination of bioactivity

Rasika R Kulkarni, Nayana S Shaiwale, Dileep N Deobagkar, Deepti D Deobagkar Molecular Biology Research Laboratory, Center of Advanced Studies, Department of Zoology, University of Pune, Pune, India Abstract: There has been rapid progress in exploring microorganisms for green synthesis of nanopar...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Kulkarni RR, Shaiwale NS, Deobagkar DN, Deobagkar DD
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2015
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/dbe69056d58240039d45a244984dd1da
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:dbe69056d58240039d45a244984dd1da
record_format	dspace
spelling	oai:doaj.org-article:dbe69056d58240039d45a244984dd1da2021-12-02T00:07:18ZSynthesis and extracellular accumulation of silver nanoparticles by employing radiation-resistant Deinococcus radiodurans, their characterization, and determination of bioactivity1178-2013https://doaj.org/article/dbe69056d58240039d45a244984dd1da2015-01-01T00:00:00Zhttp://www.dovepress.com/synthesis-and-extracellular-accumulation-of-silver-nanoparticles-by-em-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013 Rasika R Kulkarni, Nayana S Shaiwale, Dileep N Deobagkar, Deepti D Deobagkar Molecular Biology Research Laboratory, Center of Advanced Studies, Department of Zoology, University of Pune, Pune, India Abstract: There has been rapid progress in exploring microorganisms for green synthesis of nanoparticles since microbes show extraordinary diversity in terms of species richness and niche localization. Microorganisms are easy to culture using relatively inexpensive and simple nutrients under varied conditions of temperature, pressure, pH, etc. In this work, Deinococcus radiodurans that possesses the ability to withstand extremely high radiation and desiccation stress has been employed for the synthesis of silver nanoparticles (AgNPs). D. radiodurans was able to accumulate AgNPs in medium under various conditions, and process optimization was carried out with respect to time, temperature, pH, and concentration of silver salt. AgNPs were characterized using UV/vis spectroscopy, scanning electron microscopy, transmission electron microscopy, X-ray diffraction, energy-dispersive X-ray spectroscopy, and Fourier transform infrared spectroscopy. The microbially synthesized AgNPs exhibited good antimicrobial activity against both Gram-negative and Gram-positive organisms and anti-biofouling activity. Their ability to inhibit growth and proliferation of cancer cell line was also examined, and it could be seen that AgNPs synthesized using D. radiodurans exhibited excellent anticancer activity. Keywords: Deinococcus radiodurans, silver nanoparticles, anticancer, radiation resistance, antibacterial, anti-biofouling Kulkarni RRShaiwale NSDeobagkar DNDeobagkar DDDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss default, Pp 963-974 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Kulkarni RR Shaiwale NS Deobagkar DN Deobagkar DD Synthesis and extracellular accumulation of silver nanoparticles by employing radiation-resistant Deinococcus radiodurans, their characterization, and determination of bioactivity
description	Rasika R Kulkarni, Nayana S Shaiwale, Dileep N Deobagkar, Deepti D Deobagkar Molecular Biology Research Laboratory, Center of Advanced Studies, Department of Zoology, University of Pune, Pune, India Abstract: There has been rapid progress in exploring microorganisms for green synthesis of nanoparticles since microbes show extraordinary diversity in terms of species richness and niche localization. Microorganisms are easy to culture using relatively inexpensive and simple nutrients under varied conditions of temperature, pressure, pH, etc. In this work, Deinococcus radiodurans that possesses the ability to withstand extremely high radiation and desiccation stress has been employed for the synthesis of silver nanoparticles (AgNPs). D. radiodurans was able to accumulate AgNPs in medium under various conditions, and process optimization was carried out with respect to time, temperature, pH, and concentration of silver salt. AgNPs were characterized using UV/vis spectroscopy, scanning electron microscopy, transmission electron microscopy, X-ray diffraction, energy-dispersive X-ray spectroscopy, and Fourier transform infrared spectroscopy. The microbially synthesized AgNPs exhibited good antimicrobial activity against both Gram-negative and Gram-positive organisms and anti-biofouling activity. Their ability to inhibit growth and proliferation of cancer cell line was also examined, and it could be seen that AgNPs synthesized using D. radiodurans exhibited excellent anticancer activity. Keywords: Deinococcus radiodurans, silver nanoparticles, anticancer, radiation resistance, antibacterial, anti-biofouling
format	article
author	Kulkarni RR Shaiwale NS Deobagkar DN Deobagkar DD
author_facet	Kulkarni RR Shaiwale NS Deobagkar DN Deobagkar DD
author_sort	Kulkarni RR
title	Synthesis and extracellular accumulation of silver nanoparticles by employing radiation-resistant Deinococcus radiodurans, their characterization, and determination of bioactivity
title_short	Synthesis and extracellular accumulation of silver nanoparticles by employing radiation-resistant Deinococcus radiodurans, their characterization, and determination of bioactivity
title_full	Synthesis and extracellular accumulation of silver nanoparticles by employing radiation-resistant Deinococcus radiodurans, their characterization, and determination of bioactivity
title_fullStr	Synthesis and extracellular accumulation of silver nanoparticles by employing radiation-resistant Deinococcus radiodurans, their characterization, and determination of bioactivity
title_full_unstemmed	Synthesis and extracellular accumulation of silver nanoparticles by employing radiation-resistant Deinococcus radiodurans, their characterization, and determination of bioactivity
title_sort	synthesis and extracellular accumulation of silver nanoparticles by employing radiation-resistant deinococcus radiodurans, their characterization, and determination of bioactivity
publisher	Dove Medical Press
publishDate	2015
url	https://doaj.org/article/dbe69056d58240039d45a244984dd1da
work_keys_str_mv	AT kulkarnirr synthesisandextracellularaccumulationofsilvernanoparticlesbyemployingradiationresistantdeinococcusradioduranstheircharacterizationanddeterminationofbioactivity AT shaiwalens synthesisandextracellularaccumulationofsilvernanoparticlesbyemployingradiationresistantdeinococcusradioduranstheircharacterizationanddeterminationofbioactivity AT deobagkardn synthesisandextracellularaccumulationofsilvernanoparticlesbyemployingradiationresistantdeinococcusradioduranstheircharacterizationanddeterminationofbioactivity AT deobagkardd synthesisandextracellularaccumulationofsilvernanoparticlesbyemployingradiationresistantdeinococcusradioduranstheircharacterizationanddeterminationofbioactivity
_version_	1718403956461273088

Synthesis and extracellular accumulation of silver nanoparticles by employing radiation-resistant Deinococcus radiodurans, their characterization, and determination of bioactivity

Ejemplares similares